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Webinars & Tutorial Video's


For upcoming Huygens webinars, see our Webinar Schedule.

Huygens 3D Visualization

This webinar gives an overview of the many improvements that we have lately implemented in the three Huygens 3D rendering options, which are the SFP renderer, Surface Renderer, and MIP renderer. Among other features, these renderers are now GPU accelerated and as a result much faster, animation settings have been expanded, a perspective view has been added as well as a cutting plane. (14:15 min)


Getting Started


Huygens Batch Express

Batch Express. The Huygens Batch Express option allows instant deconvolution of all your acquired images independent how you acquired them. It makes use of smart templates and the high-quality deconvolution algorithms in Huygens to restore your images in a matter of seconds.(9.25 min)




Huygens Deconvolution Express

Deconvolution Express. The Huygens Deconvolution Express option makes use of smart templates and the high-quality deconvolution algorithms in Huygens to restore your images in a matter of seconds. (1.56 min)




Huygens Remote Manager


Huygens Remote Manager Webinar (February 11, 2020): This webinar will give you practical guidelines how to optimally use the Huygens Remote Manager with the Huygens Core. HRM is ideally suited for high-throughput deconvolution and allows access to Huygens via the web-browser, providing an easy multi-user access to Huygens deconvolution. HRM has an optimized interface connection to the open source OMERO server, and also allows you to perform Colocalization Analysis in batch mode and visualize deconvolution results. (26.06 min)




Huygens Batch Processor

Deconvolution Batch Processor The Huygens Batch Processor can be used to schedule the deconvolution of many images, which can be single datasets or even folders with multiple image data. This tutorial video shows you how to schedule multiple deconvolution jobs. (3.01 min)




Huygens Remote Manager


Huygens Remote Manager Webinar (February 11, 2020): This webinar will give you practical guidelines how to optimally use the Huygens Remote Manager with the Huygens Core. HRM is ideally suited for high-throughput deconvolution and allows access to Huygens via the web-browser, providing an easy multi-user access to Huygens deconvolution. HRM has an optimized interface connection to the open source OMERO server, and also allows you to perform Colocalization Analysis in batch mode and visualize deconvolution results. (26.06 min)





Super Resolution


Huygens Light Sheet Deconvolution & Fuser


Huygens Light Sheet Decon & Fuser Webinar (2020): The brand-new interactive Huygens Fuser option can fuse multiview images from a variety of different Light Sheet micrscopes and also allows the deconvolution of the images within its fusion workflow. Alignment of multiview images is very interactive and offers direct visual feedback so that you as a user are in full control. (12:30 min)




Huygens Array Detector


Array Scanning (Airyscan & SPAD) Webinar (April 30th, 2019): This webinar on HUYGENS deconvolution of Array and Airyscan Detector images to learn more about how Array-detector + HUYGENS deconvolution can help you improve your image acquisition and obtain stunning super-resolution results. We will also premier new results, demonstrating an impressive sub-90nm two-point resolution, and we will further illustrate what advantages the HUYGENS MLE-based algorithm offers over alternative restoration algorithms. (23.20 min)




Huygens Localizer


Huygens 2D & 3D Localizer webinar (19th of November 2019): Will show that 2D and 3D Single Molecule Localization Microscopy (SMLM) images acquired with PALM, STORM, GSD, or (DNA)-PAINT can be efficiently analyzed with the HUYGENS LOCALIZER. Huygens 2D & 3D Localizer is highly parallelized for CPU and GPU, and offers high-quality localization results in real-time. The easy-to-use wizard helps you with the optimization of the detection and pre/post processing settings, and includes various fitting algorithms, powerful background estimation methods, automatic drift correction, and interactive rendering and analysis. New is also the option to batch process SMLM data. (20:30 min)



Huygens STED Deconvolution


Huygens STED deconvolution and optimal processing of STED data (March 19th, 2019): This webinar will show how you can obtain the best possible results with super-resolution STED image data. STED images can be deconvolved with Huygens leading to high-quality results, and offering a huge increase in contrast and resolution. During this webinar, you will see that Huygens is optimally suited for deconvolving (2D and 3D) STED images from Leica, custom-build STED systems, and the new compact STEDYCON and STED expert line by Abberior Instruments. (42.59 min)





Visualization & Analysis


Huygens 3D Visualization


Huygens 3D Visualization Webinar (15th of October 2019): This webinar gives an overview of the many improvements that we have lately implemented in the three Huygens 3D rendering options, which are the SFP renderer, Surface Renderer, and MIP renderer. Among other features, these renderers are now GPU accelerated and as a result much faster, animation settings have been expanded, a perspective view has been added as well as a cutting plane. (14:15 min)




Huygens Object Analyzer


Object Analysis with Huygens (3rd of July 2018): Huygens deconvolution improves resolution, contrast and signal to noise ratio in the images. Because of this, the analysis of the deconvolved images is more accurate and robust. The analysis can be performed using the Huygens Object Analyzer. The interactive object analyzer tool for 3D microscopy images in Huygens Essential and Huygens Professional allows you to obtain statistics of individual objects by clicking on them, or analyzing all objects with a single button press. The image is segmented, the objects can be filtered and grouped in Regions of Interest, and the images are then analyzed for Geometrical data, Spatial location, Relation with neighbors, Relation with reference objects, Degree of overlap (colocalization).(49.57 min)




Huygens Colocalization Analyzer


Huygens Co-localization webinar (19th June 2018): Colocalization studies provide an important insight on several biological processes, and Huygens can help you in getting the most accurate colocalization results. Huygens deconvolution improves resolution, contrast and signal to noise ratio, reducing the effect of Blur And Noise in Colocalization studies. Our Restoration Options also correct the images for the other aberrations, getting them ready for an accurate analysis. For what concerns the analysis, the Colocalization Analyzer works more at the level of the whole image, and the Object Analyzer also provides colocalization measurements at the object level. In this webinar, we will discuss: image restorations before co-localization analysis and why, a brief introduction of the co-localization coefficients, how to use the Huygens co-localization Analyzer (demo).(36.21 min)




Huygens Surface Renderer

Surface renderer. This tutorial video shows you how to use the Huygens Surface Renderer to represent your microscopy data in a convenient way to clearly see separated volumes, with Iso Surface rendering (4.14 min)




Huygens Object Tracker

The Object Tracker. A tutorial about the Huygens Object Tracker, giving a short demonstration about the Huygens Object Tracker and Track Analyzer. (4.02 min)




Huygens Movie Maker

Movie Maker. A tutorial about the Huygens Movie Maker, giving a short demonstration on how to use the Movie maker and showing some examples made with the tool. (6.41 min).





Image Restoration


True Deconvolution

True Deconvolution Webinar The essence of using Deconvolution is to reverse the Convolution process, which is how a microscope distorts images. Since convolution involves object blurring by the microscope type-specific point spread function (PSF), it is crucial that this PSF is accurately calculated or measured, and included in a deconvolution procedure with the best possible algorithms. These are currently MLE-based as implemented by Huygens. In this webinar, we will explain image acquisition and what true deconvolution includes. After this webinar, you have learned about the potential limitations of other image enhancement techniques and know how to obtain the most reliable and accurate results from your valuable image data. (27:31 min).




Huygens Stitcher


Stitching in Huygens Webinar (17th September 2019): During this webinar will discuss the Huygens Stitching & Deconvolution wizard. The Stitching & Deconvolution option is GPU accelerated, and offers a unique easy-to-use wizard for combined high-quality stitching for all types of microscopy data with the additional option to deconvolve the images, and to correct for vignetting issues. Vignetting correction can be performed manually and in a full automated fashion. The Stitching & Deconvolution Wizard is available for Huygens Essential and Huygens Professional. (14.22 min)




Huygens PSF Distiller


The PSF Distiller. The Huygens PSF Distiller can be used with imaged beads to measure a point spread function that can then be used with image deconvolution. This tutorial video shows the ins and outs of PSF distillation. (7.31 min)




Huygens Object Stabilizer


The Object Stabilizer. The Huygens Object Stabilizer can measure and correct for cell motion, thermal drift, shaking, and other types of movement (x-y-z translation and axial rotation). Both the measurement and subsequent stabilization are done in 3D and at sub-pixel level. The Stabilizer not only stabilizes 2D or 3D time series, but it also allows the alignment of z-slices within a 3D stack. (4.39 min)




Huygens Chromatic Shift Corrector


The Chromatic Aberration Corrector. The Huygens Chromatic Aberration Corrector can correct chromatic shift on images due to various reasons. This tutorial video shows how to use the Chromatic Aberration Corrector. (4.04 min)




Huygens Crosstalk Corrector


The Crosstalk Corrector. The Huygens Crosstalk Corrector estimates and corrects for crosstalk, also known as bleedthrough. Crosstalk can occur if there is spectral overlap between different channels, and signal is recorded from a specific dye whereas the detector is already reserved for another dye. Crosstalk in multi channel images up to 32 channels can be easily estimated, visualized, and corrected with this tool. (3.24 min)




You are welcome to test Huygens for free with all the available Huygens options.

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