HUYGENS Webinar invitation:
This broadcasted webinar about Huygens LOCALIZER can still be viewed. You can just register or send an email with your contact details to sales at svi.nl for access.
Huygens 2D & 3D LOCALIZER webinar (19th November 2019).Huygens 2D & 3D Localizer is a very user-friendly stand-alone software package for analysing SMLM images acquired with PALM, STORM, GSD, or (DNA)-PAINT. Huygens Localizer is fast due to its efficient use of CPU and GPU, and offers high-quality localization results in real-time. The easy-to-use wizard with its extensive help functions supports optimal detection and pre/post processing. Various fitting algorithms, powerful background estimation methods, automatic drift correction, and interactive on-the-fly rendering and analysis quartantee optimal results. Latest additions are 3D SMLM analysis with Huygens renowed PSF distiller for accurate Z calibration, and batch processing of SMLM data.
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Huygens 3D rendered SFP image of TOM20 mitochondrial antibody staining was acquired with a SMLM system and analyzed with Huygens 2D and 3D Localizer using for Z calibration an astigmatic image of 100nm beads. Imaging was performed by Marko Lampe (Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Heidelberg, Germany) using a Leica SR GSD. Thanks to Christoph Spahn and Mike Heilemann (Goethe University Frankfurt, Frankfurt am Main, Germany) for preparing the TOM20 DNA-PAINT sample .