Scientific Volume Imaging

First Name *{$f_735}

Last Name *{$f_736}

E-mail Address *{$f_737}

Institution/Company *{$f_738}

Country *{$f_739}

Question 1:* What file format should I use if I need to preserve the dynamic range, the metadata, and the possibility to open it with other (open source) software?

• A: Tiff
• B: JPEG
• C: ICS or ICS2
• D: A,B and C are all possible

Answer: {$f_740}

Question 2:* What is the difference between the Deconvolution Wizard and the Deconvolution Express?

• A: Deconvolution Express gives you the most optimal settings.
• B: Deconvolution Wizard offers the chance to load a measured PSF.
• C: Deconvolution Wizard allows you to fine tune more parameters.
• D: Both B and C are correct.

Answer: {$f_741}

Question 3:* Which microscopic parameter(s) is/are important to set correctly if you want to make sure Huygens corrects for spherical aberration?

• A: Coverslip position.
• B: Imaging direction.
• C: Coverslip position, imaging direction and refractive indices of lens and sample medium.
• D: Coverslip position, imaging direction, refractive indices of lens and sample medium and channel label.

Answer: {$f_742}

Question 4:* What does the contrast option 'Displayed region' mean?

• A: It scales the contrast range to the intensities of the area that is currently in view.
• B: It links the contrast range over all channels.
• C: It resets the contrast.
• D: It selects the best channel to visualize.

Answer: {$f_743}

Question 5:* How do you draw circles in the Twin Slicer?

• A: Under the 'Contrast' tab.
• B: You automatically draw circles by dragging across the view.
• C: By dragging the middle mouse button.
• D: You right click on the view and select 'Draw circles' and then drag across the view.

Answer: {$f_744}

Question 6:* What is NOT a feature of the new Quality Control option?

• A: Detecting potential issues in your image data.
• B: Estimate the correct deconvolution parameters.
• C: Links to Huygens options that correct for the detected issues.
• D: Provide background introduction about the typical acquisition issues.

Answer: {$f_745}

Question 7:* Via which of the following arrangements can you access Huygens on multiple workstations:

• A: When using Huygens Remote Manager.
• B: Huygens node-locked license with remote display.
• C: Huygens Everywhere with one user.
• D: Huygens Everywhere with multiple users.
• E: All of the above.

Answer: {$f_746}

Question 8:* Can you login when all of the concurrences/seats are in use?

• A: No.
• B: Yes, if you are an admin you can log-out other users.
• C: Yes, if you are an admin you can log-out other users, or when you have one seat you can take over your own seat that is logged in on another workstation.

Answer: {$f_747}

Question 9:* What is the main/basic result of the Huygens Localizer (from which other output/results are derived)?

• A: Localization table containing the positions of all molecules/ fluorophores found.
• B: A high resolution (rendered) image of the object under the microscope.
• C: 3D surface rendered objects.

Answer: {$f_748}

Question 10:* What is the STED Saturation factor?

• A: A factor that describes what percentage of the fluorophores are being saturated and cannot be depleted.
• B: A factor describing the amount by which the fluorescence is suppressed by the STED beam.
• C: A factor that describes how much laser power is used for the stimulated emission depletion.
• D: A factor that tells you the ratio between axial and lateral STED laser power.

Answer: {$f_749}

Question 11:* Will Chromatic Aberration artifacts typically increase or decrease overlap between channels?

• A: Only increase overlap
• B: Only decrease overlap
• C: Both, but an increase in overlap is much more likely.
• D: Both, but a decrease in overlap is much more likely.

Answer: {$f_750}

Question 12:* What sort of aberrations between channels can Huygens restore? 1) Linear shifts, 2) scaling along each axis, 3) nonlinear shifts, 4) rotation around all axis, 5) inversions

• A: 1 and 2
• B: 1, 2 and 5
• C: 1, 3 and 4
• D: 1, 2, 3 and 4
• E: 1, 2, 4 and 5
• F: 1, 2, 3, 4 and 5

Answer: {$f_751}

Question 13:* Can Huygens correct for crosstalk and autofluorescence in one calculation?

• A: es, crosstalk and autofluorescence cause similar correlations between channels and are therefore easily removed with one calculation.
• B: Yes, but this is incredibly difficult to determine as the correlation between channels is significantly distorted by the autofluorescence.
• C: No, crosstalk and autofluorescence need to be removed by two totally different matrix multiplications that cannot be mathematically combined.

Answer: {$f_752}

Question 14:* How can Huygens currently correct for autofluorescence (we are working on a tailored Huygens solution)?

• A: By specifying the fluorophores excitation and emission wavelengths together with the spectrum of autofluorescence in the microscopy parameter editor.
• B: Train an AI algorithm with 50 to 100 images with manually selected high autofluorescence regions. After training the resulting model serves as a template in the Crosstalk Corrector.
• C: By measuring the autofluorescence in a separate channel and construct a crosstalk matrix that removes the autofluorescence from the real channels.
• D: Not. Autofluorescence correction is not possible in the current version of Huygens (24.04).

Answer: {$f_753}

Question 15:* Where can you find the interactive tutorials for the Object Analyzer?

• A: Under the "Help" top menu in the Object Analyzer.
• B: In the Workflow Designer.
• C: In the Huygens manuals.

Answer: {$f_754}

Question 16:* Why is Otsu's method not always sufficient for segmenting nuclei?

• A: It does not work on 3D data.
• B: It cannot separate nuclei that touch each other.
• C: Both A and B are true.
• D: It is too slow for large datasets.

Answer: {$f_755}

Question 17:* Why is it important to do Bleaching Correction before Tracking?

• A: The intensity of the objects and background should not change too much for detecting objects.
• B: Tracking the objects is more difficult because of dimming intensities.
• C: Both A and B are true.
• D: Bleaching Correction isn't needed for Tracking.

Answer: {$f_799}

*Would you like to recieve a certificate of the exam?

Answer: {$f_801}