Huygens Array Detector Confocal Optical Option
Supports Zeiss Airyscan®, Fast Airyscan®, nanoSPAD and others!
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The new Huygens Array Detector Confocal deconvolution is the first of its kind to be integrated in a powerful GPU accelerated MLE-algorithm, and is now available with the new Huygens Suite 18.10. This Array Detector optical option is specifically developed to restore images acquired on multi-detector confocal systems, including the well-received and popular Zeiss Airyscan system (1). Thanks to SVI’s newly developed smart multi-detector algorithms, the restoration of raw Array Detector images with Huygens is very efficient, and allows scientists to obtain reliable super-resolution images in which objects at 100nm distance can be distinghuised. More importantly, the unique Huygens Array Detector Super-sampling mode fullfils a wish common to many microscopist as it allows fast image acquisition with less bleaching and phototoxicity without compromising the image contrast and super-resolution gain.
Reach 100nm resolution
Recent tests with GattaQuant nanorulers (100, 120, 140 nm) confirm 100nm two-point resolution is achievable.
Huygens Super-sampling mode permits faster imaging without compromising resolution and contrast.
Multiple detector layouts are supported, including Zeiss (Fast) Airyscan super-resolution, nanoSPAD layouts, and other generic layouts.
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About array-detector confocal systemsWith an array-detector confocal system, the conventional pinhole and single detector in a confocal system is replaced by an array of multiple detectors. Each individual detector acts as a small pinhole, where all but the central detector are slightly displaced with respect to the the original pinhole center. The signal from each detector is used to build up it's own image, where each image is a slightly shifted version compared to the central detector image. The total (summed) signal collected by all detectors is comparable to a large pinhole in 'classic' confocal. As a result, an array-detector confocal system has the high-signal benefits from a large pinhole confocal system, while simultaneously benefiting from the high- resolution aspect of a small pinhole confocal system.
The Huygens Array Detector Optical Option is the solution to obtain high resolution and contrast in array detector data, such as images acquired on the Zeiss Airyscan system. Next to supporting the various Zeiss Airyscan modes, the Huygens Array Detector Confocal Optical Option also supports other multi-detector layouts. This includes nanoSPAD detector layouts, or other generic mulit-detector layouts.
Huygens Array Detector deconvolution gallery
With 80nm sampling in X and Y, you can resolve 140nm GattaQuant (2) nanorulers with the special superXY mode. Shown are MIPs. The right image is a sum of the unprocessed raw data from all detectors. Images obtained with Zeiss Airyscan microscope kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK
Huygens’ unique MLE algorithm - high quality and reliabilityThe Array Detector confocal option in Huygens is able to combine multiple detector images in a classic maximum-likelihood estimation (CMLE) framework to yield a super-resolution image with increased contrast and resolution as compared to images from conventional confocal-based system.
Image formation in fluorescence microscopy is well understood, and can be accurately described with well-known mathematical equations. Image restoration is the inverse problem of trying to solve the equation as best as possible. A maximum-likelihood estimation (MLE) image restoration approach, as implemented in the Huygens Software, is known to be best approach to accurately solve the image restoration problem in microscopy. For the image restoration to be successful, an MLE approach requires so called 'a priori' information, which is information about the image formation process that is well known. The more 'a priori' information you can put into the equation, the better and more reliable the image restoration result will become.
Fast imaging + Fast high-quality Huygens Super-sampling deconvolutionA particular useful property of array-detector confocal systems, is that the Nyquist criterion is much less strict compared to regular confocal. This allows for the use of larger pixel sizes, which in turn allows for faster imaging, less photo-bleaching and/or imaging a much larger field of view in the same amount of time as conventional confocal. Deconvolution of such data can be applied with the specifically designed Huygens Super-sampling mode without any trade-off in SNR and while keeping the super-resolution aspect.
In 2016 Carl Zeiss introduced the Fast Airyscan mode, where 4 lines can be scanned simultanously. Huygens also offers deconvolution for Fast Airyscan images.
1. Carl Zeiss Microscopy GmbH.
2. GattaQuant GmbH. For more information, see the GattaQuant website.