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Scientific Volume Imaging B.V.

Scientific Volume Imaging

Deconvolution - Visualization - Analysis


™Huygens Array Detector Confocal Deconvolution

Supports Zeiss LSM710/780/800/880 (Fast)Airyscan-1®, LSM900/980 Airyscan-2®, and nanoSPAD images!

For information about support for Array Detector STED images, see our STED product page
Confocal
Huygens Deconvolved superXY
Multi-detector confocal images profit from the high-signal of a large pinhole and high-resolution aspect of a small pinhole, however each detector has a slightly shifted version of the central detector image and thus the final image is still a blurred compromise. Using Huygens revolutionary Array Detector deconvolution, unprecedented high contrast and resolution can be achieved as the position information of each individual detector is integrated in Huygens' famous maximum-likelihood estimation (MLE) framework.

Huygens' MLE-based GPU-accelerated algorithms work with any array detector confocal system, including Zeiss Airyscan 1 and 2, enabling reliable super-resolution images that distinguish objects as close as 70nm and 90nm (see below).

Huygens’ unique Array Detector Super-Sampling mode for Airyscan and FastAiryscan allows faster scanning while enhancing resolution and contrast, reducing bleaching and photo-toxicity, and providing more image detail—fulfilling a key need for biologists.

Image description HeLa cells (MIP) imaged with Zeiss LSM 880 Airyscan system and deconvolved with the Huygens Array detector using the superXY mode. Cells were stained with anti-Ki67 and secondary-Alexa488 (magenta), Phallodin-TMR (White), and anti-alphaTubulin and secondary Abberior Star Red (shown in green). Image kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK).

Reach 90nm resolution

Tests with a range of GattaQuant nanorulers confirm 90nm two-point resolution is achievable.

Unique Super-sampling

Huygens Super-sampling mode combines the option of faster imaging and improving resolution and contrast.

Derive experimental PSFs from beads

Huygens PSF Distiller supports Quality Control measurements and deconvolution with experimental PSFs.

Testimonials

We are quite impressed with the Huygens Array Detector results. Dr. Glyn Nelson, microscopist in the Bioimaging Unit, Campus for Ageing and Vitality, Newcastle University, UK.
Huygens array detector deconvolution has allowed us to improve the resolution of Airyscan images beyond their normal limits. Dr. Stephen Freeman, light microscopy platform expert at GIGA Liege, Liège Université, Belgium.

Faster than fast imaging: Huygens Super-sampling deconvolution

A wish of many microscopists comes true: Get more resolution and image faster using Huygens Super-sampling mode. This unique deconvolution mode makes the Nyquist criterion for array detector imaging much less strict, because the intensity and precise position information of all detectors is efficiently combined by Huygens to create a super-sampled deconvolved image. You can image now at a much higher speed using a larger pixel size and still improve resolution up to the level of distinguishing objects that are only 70nm apart. Obtain significantly better image quality, image a larger field of view, and decrease photo-bleaching and -toxicity drastically with Huygens Array Detector deconvolution. SuperXY 70nm

Huygens unique SuperXY mode clearly separates objects that are 70 nm apart. This two channel image of GATTAquant SIM140BYB nanorulers was acquired with the Zeiss Airyscan Fast Mode at 27 nm xy pixel size, which would not be sufficient to resolve 70nm spaced objects. By making optimal use of the signal and precise positiono information of all detectors in the Array, Huygens deconvolution is able to create a super-sampled image. Microscopy data was kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK

A clear 90nm two-point super resolution

Huygens' unique MLE deconvolution uses the information available from the array of detectors to reach a clear 90nm two-point resolution. Airyscan90nm STED90B

GATTAquant STED90B nanorulers, consisting of carefully folded DNA origami with fluorophores spaced at a calibrated distance of 90nm, were imaged with a Zeiss Airyscan and deconvolved with Huygens Array detector MLE algorithm. A two-point resolving capbility of at least 90nm is easily reached.Images were kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK

Detector position Quality Control

Quality Control tool which automatically calculates and visualizes the relative positions and intensities of each used detector with respect to the central detector. By making optimal use of the position and intensity information, Huygens Array (Airyscan) Detector deconvolution is able to separate objects less than 90nm apart and produce reliable and quantifiable results.

QC Detector

Use in research

Mostafa F. ElMaghraby, Peter Refsing Andersen, Florian Pühringer et al., A Heterochromatin-Specific RNA Export Pathway Facilitates piRNA Production. Airyscan images were deconvolved with Huygens. Cell 178, 964-979 (2019)

For more, see Scientific Publications

Because of its fast imaging and low phototoxicity and bleaching, Array Detector microscopy is well fit for live-cell imaging. Huygens can then be used to stabilize time series and to track object movement over time.

Object Stabilizer Object Tracker

More information

Introduction to deconvolution Huygens Deconvolution software Deconvolution images

Neurons Large
This Fast Airyscan (Zeiss) image of a dendrite was deconvolved with Huygens unrivalled SuperY deconvolution, which combines the information from all available detectors optimally to generate a super-resolution result image of unprecedented quality. Left image shows the sum of all 16 detectors, which is basically the confocal version. Right image: Huygens unmatched SuperY deconvolution. C/o Giovanna Expósito, Servicio de Imagen, Instituto de Neurociencias CSIC-UMH.'' See more: Images in the field of Neurosciences
GATTA140B Figure
Array Detector deconvolution with Huygens SuperXY mode allows you to sample with 80nm pixel size and still be able to resolve 140nm spaced fluorophores on GattaQuant nanorulers. Note that 2x2 pixels covering a 160x160nm area would normally not be able to separate objects that are 140nm apart. Shown are MIPs. Image obtained with Zeiss Airyscan microscope kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK