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Cell Biology

We offer the complete software solution for microscopy imaging.



Huygens solves all major problems that occur during imaging

    • Deblurring
    • Denoising
    • Better resolution
    • Crosstalk correction - make better use of your available labelling dyes!
    • Microscope misalignment correction - chromatic and spherical

Quantify your data and visualise publication ready

Trusted & easy - 25 years of pioneering experience

For all microscope types - Widefield, Confocal, Airyscan, STED & More


Image description:
HeLa cells were treated with Nocodazole on ice for 40 min, and then washed and incubated with complete media at 37ºC for 5 min. After fixation, cells were stained by immunofluorescence. Image was acquired in a Zeiss Axioimager Z2 epifluorescence microscope, deconvolved with Huygens software and visualized with Huygens 3D MIP renderer. Image kindly provided by Dr. Claudia Florindo, CBME/FCT - Institute for Biotechnology & BioEngineering, University of Algarve, Portugal.

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Huygens Deblurring

HUYGENS DECONVOLUTION brings many advantages, to name a few: deblurring, denoising, better resolution and quantitative image analysis! Blurring is recognised as a haze over your images and is mostly caused by out-of-focus light. HUYGENS DECONVOLUTION deblurs your image by restoring out-of-focus light to the fluorophore center. Huygens deblurring works for all microscope types, but is especially noticeable for widefield. Check out our HUYGENS DECONVOLUTION for widefield! The solution to upgrade your everyday imaging needs.

Image description:
Stained structures (kinetochore complex) involved in cell division were imaged with a DeltaVision Widefield microscope. Huygens deconvolution makes segmentation more easy and reliable by correcting the out-of-focus signal and noise. This led to a 30-fold signal increase and the indentification of objects that are at 220nm proximity. Image kindly provided by Dr. Livio Kleij (facility) and Martijn Vroomans. Medical Oncology, UMC Utrecht, The Netherlands.

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Raw Widefield
Huygens Deconvolved




Better resolution & denoising

HUYGENS DECONVOLUTION reveals the truth behind your images. Using knowledge of your microscope performance it restores the object that underlies your image. Enable longer life-cell acquisition using lower excitation and Huygens denoising. Or upgrade to premium resolution on your existing system using HUYGENS DECONVOLUTION. Check out our solutions for Confocal microscopes!


Image description:
Confocal image showing basal bodies of the cilia in a paramecium. Image was provided by A. Aubusson-Fleury, CNRS, Gif sur Yvette, Paris.

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Raw Confocal
Huygens Deconvolved




Better quantitative results

We value reliable image restoration over just making the images look good, see also our quantitative imaging white paper. HUYGENS DECONVOLUTION enables better quantitative results as you can analyze the underlying object and not a blurred version. Check out our Object Analyzer for object segmentation and accurate statistics. Or try our Colocalisation Analyzer and get more insight in your data.

Image description:
3D volume analysis of kinetochore complex structures involved in cell division. Deconvolution with Huygens was shown to be critical for the segmentation of the objects. Image kindly provided by Livio Kleij (facility) and Martijn Vroomans. Medical Oncology, UMC Utrecht, The Netherlands.

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Use Huygens everywhere 

HUYGENS EVERYWHERE is a new way to use Huygens on your existing product based on a monthly subscription.
Already have a permanent license? Similar to a maintenance contract, you receive the latest updates and service while using Huygens everywhere.
Are you part of a larger facility? Check out our floating options for many users centralised on a big server or distributed on your user pc’s.


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Huygens Everywhere



Huygens can do much more!




Cell Biology Image Gallery



Malide COS7 STED Tubulin Depth Color Coded Mitochondria
This STED image of COS-7, a fibroblast-like cell line derived from monkey kidney tissue, shows mitochondria marked by Tom20 staining, (green), and cytoskeletal protein tubulin color-coded for depth. The mitotic spindle is clearly visible in the center. This Leica SP8 STED 3X image was deconvolved with Huygens. Image courtesy of Dr. Daniela Malide, NIH - National Heart, Lung and Blood Institute (NHLBI), United States.


Fire   SVI Contest
A primary hepatocyte in 3D cell culture. After cytokine treatement, the primary hepatocytes undergo a process known as epithelial to mesenchymal transition (EMT). The cell exhibits a mesenchymal morphology with actin stress fibers spanning the cell body. The actin cytoskeleton is visualized by phalloidin staining, DNA is stained with Hoechst33528. Confocal image was acquired with the Nikon A1R. Devonvolution is performed with Huygens Essential. Image courtesy of Dr. Marlies Mürnseer, Heidelberg University, Germany.



Glyn Nelson Mito.resized
Mitochondrial dysfunction in senescent human fibroblasts. This Leica widefield image shows the mitochondrial network (mitotracker green), it's activity (TMRM, red) and cell nuclei (Hoechst, blue). The z stack was deconvolved in Huygens Essential and rendered using SFP. Individual cells can be seen to have varying levels of energy production (red intensity), as well as intracellular variation between individual mitochondrial networks. Image courtesy of Dr. Glyn Nelson, University of Newcastle upon Tyne, United Kingdom.



A Story Of Ice And Fire   SVI Contest
Differentiated hepatocytes in 3D cell culture Primary hepatocytes in a 3D cell culture matrix. The cells are differentiated and maintain the organotypic polarized morphology and functionality. The actin cytoskeleton exhibits fine fibrils, supporting the bile-canaliculi which form at cell-cell interfaces. Confocal image was acquired with the Nikon A1R. Devonvolution is performed with Huygens Essential. Image courtesy of Dr. Marlies Mürnseer, Heidelberg University, Germany.


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Image shows a tetranucleated cell with DNA stained with DAPI (in cyan), the microtubules labled with a-tubulin antibody (in red) and the centrosomes detected with y-tubulin antibody in green. The image was taken with a Zeiss Axioimager Z2 epifluorescence microscope, deconvolved with Huygens software, and rendered with Huygens Surface Renderer. Image courtesy of Dr. Claudia Florindo, CBME/FCT - Institute for Biotechnology & BioEngineering, University of Algarve, Portugal.