Cell Biology
We offer the complete software solution for microscopy imaging.
Huygens solves all major problems that occur during imaging
- Deblurring
- Denoising
- Better resolution
- Crosstalk correction - make better use of your available labelling dyes!
- Microscope misalignment correction - chromatic and spherical
Quantify your data and visualise publication ready
Trusted & easy - Over 25 years of pioneering experience
For all microscope types - Widefield, Confocal, Airyscan, STED & More
People in our lab who do confocal microscopy [..] are really impressed by the program! We find it very important for our research with yeast cells and bacteria
Dr. Christina Schlatterer, University of Konstanz, Germany.
Dr. Christina Schlatterer, University of Konstanz, Germany.
Image description:
HeLa cells were treated with Nocodazole on ice for 40 min, and then washed and incubated with complete media at 37ºC for 5 min. After fixation, cells were stained by immunofluorescence. Image was acquired in a Zeiss Axioimager Z2 epifluorescence microscope, deconvolved with Huygens software and visualized with Huygens 3D MIP renderer. Image kindly provided by Dr. Claudia Florindo, CBME/FCT - Institute for Biotechnology & BioEngineering, University of Algarve, Portugal.
HeLa cells were treated with Nocodazole on ice for 40 min, and then washed and incubated with complete media at 37ºC for 5 min. After fixation, cells were stained by immunofluorescence. Image was acquired in a Zeiss Axioimager Z2 epifluorescence microscope, deconvolved with Huygens software and visualized with Huygens 3D MIP renderer. Image kindly provided by Dr. Claudia Florindo, CBME/FCT - Institute for Biotechnology & BioEngineering, University of Algarve, Portugal.
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Huygens Deblurring
HUYGENS DECONVOLUTION brings many advantages, including deblurring, noise correction, better resolution and more reliable quantitative image analysis! Blurring is recognized as a haze over your images and is mostly caused by out-of-focus light. HUYGENS DECONVOLUTION deblurs your image by restoring out-of-focus light to the fluorophore center. Huygens deblurring works for all microscope types, but is especially noticeable for widefield. Check out our HUYGENS DECONVOLUTION for widefield! The solution to upgrade your everyday imaging needs.I am a PhD student at the final stages and your software has helped clear some problems that I have been trying to fix for a while!
Farhana Chowdhury, PhD student, Neural Tissue Engineering, Keele University, UK.
Farhana Chowdhury, PhD student, Neural Tissue Engineering, Keele University, UK.
Image description:
Stained structures (kinetochore complex) involved in cell division were imaged with a DeltaVision Widefield microscope. Huygens deconvolution makes segmentation more easy and reliable by correcting the out-of-focus signal and noise. This led to a 30-fold signal increase and the indentification of objects that are at 220nm proximity. Image kindly provided by Dr. Livio Kleij (facility) and Martijn Vroomans. Medical Oncology, UMC Utrecht, The Netherlands.
Stained structures (kinetochore complex) involved in cell division were imaged with a DeltaVision Widefield microscope. Huygens deconvolution makes segmentation more easy and reliable by correcting the out-of-focus signal and noise. This led to a 30-fold signal increase and the indentification of objects that are at 220nm proximity. Image kindly provided by Dr. Livio Kleij (facility) and Martijn Vroomans. Medical Oncology, UMC Utrecht, The Netherlands.
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Huygens Deconvolved
Better resolution & noise correction
HUYGENS DECONVOLUTION reveals the truth behind your images. Using knowledge of your microscope performance it restores the object that underlies your image. Enable longer live cell acquisition by using lower excitation and then deconvolving with Huygens to correct noise. Or upgrade to premium resolution on your existing system using HUYGENS DECONVOLUTION. Check out our solutions for Confocal microscopes!I thank you all for providing such as wonderful tool that enriching our knowledge by revealing important detail information in cell biology.
Dr. Shuoshuo Wang from the Weizmann Institute of Science, Israel.
Dr. Shuoshuo Wang from the Weizmann Institute of Science, Israel.
Image description:
Confocal image showing basal bodies of the cilia in a paramecium. Image was provided by A. Aubusson-Fleury, CNRS, Gif sur Yvette, Paris.
Confocal image showing basal bodies of the cilia in a paramecium. Image was provided by A. Aubusson-Fleury, CNRS, Gif sur Yvette, Paris.
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Huygens Deconvolved
Better quantitative results
We value reliable image restoration over just making the images look good, see also our quantitative imaging white paper. HUYGENS DECONVOLUTION enables better quantitative results as you can analyze the underlying object and not a blurred version. Check out our Object Analyzer for object segmentation and accurate statistics. Or try our Colocalisation Analyzer and get more insight in your data.Image description:
3D volume analysis of kinetochore complex structures involved in cell division. Deconvolution with Huygens was shown to be critical for the segmentation of the objects. Image kindly provided by Livio Kleij (facility) and Martijn Vroomans. Medical Oncology, UMC Utrecht, The Netherlands.
3D volume analysis of kinetochore complex structures involved in cell division. Deconvolution with Huygens was shown to be critical for the segmentation of the objects. Image kindly provided by Livio Kleij (facility) and Martijn Vroomans. Medical Oncology, UMC Utrecht, The Netherlands.
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Use Huygens everywhere
HUYGENS EVERYWHERE is a new way to use Huygens on your existing product based on a monthly subscription.Already have a node-locked license? Similar to a maintenance contract, you receive the latest updates and service while using Huygens everywhere.
Are you part of a larger facility? Check out our floating options for many users centralised on a big server or distributed on your user pc’s.
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Huygens can do much more!
Cell Biology Image Gallery
This STED image of COS-7, a fibroblast-like cell line derived from monkey kidney tissue, shows mitochondria marked by Tom20 staining, (green), and cytoskeletal protein tubulin color-coded for depth. The mitotic spindle is clearly visible in the center. This Leica SP8 STED 3X image was deconvolved with Huygens. Image courtesy of Dr. Daniela Malide, NIH - National Heart, Lung and Blood Institute (NHLBI), United States.
A primary hepatocyte in 3D cell culture. After cytokine treatement, the primary hepatocytes undergo a process known as epithelial to mesenchymal transition (EMT). The cell exhibits a mesenchymal morphology with actin stress fibers spanning the cell body. The actin cytoskeleton is visualized by phalloidin staining, DNA is stained with Hoechst33528. Confocal image was acquired with the Nikon A1R. Devonvolution is performed with Huygens Essential. Image courtesy of Dr. Marlies Mürnseer, Heidelberg University, Germany.
Mitochondrial dysfunction in senescent human fibroblasts. This Leica widefield image shows the mitochondrial network (mitotracker green), it's activity (TMRM, red) and cell nuclei (Hoechst, blue). The z stack was deconvolved in Huygens Essential and rendered using SFP. Individual cells can be seen to have varying levels of energy production (red intensity), as well as intracellular variation between individual mitochondrial networks. Image courtesy of Dr. Glyn Nelson, University of Newcastle upon Tyne, United Kingdom.
Differentiated hepatocytes in 3D cell culture Primary hepatocytes in a 3D cell culture matrix. The cells are differentiated and maintain the organotypic polarized morphology and functionality. The actin cytoskeleton exhibits fine fibrils, supporting the bile-canaliculi which form at cell-cell interfaces. Confocal image was acquired with the Nikon A1R. Devonvolution is performed with Huygens Essential. Image courtesy of Dr. Marlies Mürnseer, Heidelberg University, Germany.
Image shows a tetranucleated cell with DNA stained with DAPI (in cyan), the microtubules labled with a-tubulin antibody (in red) and the centrosomes detected with y-tubulin antibody in green. The image was taken with a Zeiss Axioimager Z2 epifluorescence microscope, deconvolved with Huygens software, and rendered with Huygens Surface Renderer. Image courtesy of Dr. Claudia Florindo, CBME/FCT - Institute for Biotechnology & BioEngineering, University of Algarve, Portugal.