Check the image microscopy parameters
This article explains when and how to use the correct Microscopy Parameters when deconvolving and analysing images in Huygens Essential.
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The Microscopic Parameter Wizard in Huygens Essential shows a summary of the image parameters.
(Click on the image for a larger view).
Correction at any time
You can see and correct the image parameters not only at this deconvolution stage, but also at any time by right-clicking (see Mouse Buttons In Mac Aqua) on the image thumbnail and selecting Show parameters or Correct parameters. Correcting will pop up a window in which the entry fields change colors as explained above.
For more details about these parameters, see → Microscopic Parameters.