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SPIM/Light Sheet Fusion & Deconvolution Wizard

The Fusion & Deconvolution Wizard for SPIM/LightSheet images is available in Huygens Essential and Huygens Professional since version 17.04. This Wizard which can be found under 'Deconvolution' in the Huygens taskbar.

Use it to deconvolve and fuse multiple views from different directions of the same specimen into one single superior image. By integrating the deconvolution into the fusion process, improved results can be obtained, while significantly simplifying and optimizing the post-processing steps.

Examples

Below you can find two examples of images that have been deconvolved and fused with the Huygens software in the Deconvolution & Fusion Wizard.



Denis Ressnikoff from the University Claude Bernard Lyon 1, in France was so kind to share with us his Zeiss Z1 data. His images were acquired at 360 degrees with 45 degrees steps. Deconvolution and fusion of this data by Huygens clearly improves the contrast of the images.



This second Fusion & Deconvolution Wizard example shows two images acquired separately with illumination from opposing sides with the Zeiss Z1. The images have been kindly provided by Shingo Nagawa from the Chinese academy of Sciences. Images show a leaf from the plant Arabidopsis deconvolved and fused with Huygens. A detail of the stomata and a line profile through these structures show a significant boost in resolution and signal to noise ratio.

Huygens Fused + Deconvolved
Raw SPIM


Manual & More information

If you would like to have more information on the SPIM/Light Sheet Fusion & Deconvolution Wizard and how to use it, please take a look at our manual which can be downloaded here. You can also request a free trail license in case you would like to try the the Fusion & Deconvolution Wizard or any other option available within the Huygens software.

Contact Information

Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands


Phone: +31 (0)35 64216 26
Fax: +31 (0)35 683 7971
E-mail: info at svi.nl

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