Loading...
 

Huygens SPIM/light sheet Optical Option

Selective Plane Illumination Microscopy (SPIM)/Light Sheet images can be deconvolved with a Huygens optical option that takes the specific optical properties of these microscopes into account.

SPIM and Light Sheet Fluorescence Microscopy combine fast 3D image acquisition with optical-sectioning by focusing with an excitation objective a thin laser light-sheet into the specimen. This light sheet is perpendicularly positioned with respect to the objective and detector which collect the emitted fluorescent signal as a 2D image. Typically, the specimen is moved along the optical axis to record a three dimensional stack. Multiple stacks can be acquired from different angles ad aligned to account for possible light loss/shading effects.
The SPIM/Light Sheet technique is very well suited for imaging living specimens since the illumination is restricted to the focal plane which minimizes photodamage and improves contrast. Also no point-scanning is needed shortening the acquisition time significantly.

The Huygens SPIM/Light Sheet optical option allows deconvolution of large data sets using the robust Huygens deconvolution algorithms. Huygens SPIM/Light Sheet deconvolution reduces noise and blurring, and takes depth-dependent spherical aberration correction into account. More information on how to deconvolve SPIM/Light Sheet images in Huygens can be found on SPIM deconvolution.

Features

  • Improve the quality and resolution of your SPIM images using state of the art deconvolution methods.
    • PSF adapted to local light sheet thickness.
  • Quickly process very large data sets.
    • GPU support
  • Visualize and quantitatively analyse your data after deconvolution.

Huygens Deconvolution of high-resolution MuVi SPIM data::


Huygens deconvolution of high resolution SPIM data. GFP labeled yolk granules in a C. elegans one-cell stage embryo before (left) and after deconvolution with the CMLE algorithm using a theoretical SPIM point-spread-function. SPIM raw data used by permission from Dr. Uros Krzic, Dr. Lars Hufnagel, and Dr. Yury Belyaev, European Molecular Biology Laboratory, Heidelberg.



Huygens MIP-rendered and deconvolved SPIM data from a Leica DLS microscope



Maximum Intensity Projection of a raw (left) and deconvolved (right) 3D image from mouse blastocysts acquired with a Leica Digital Light Sheet microscope. Deconvolution was performed with the CMLE algorithm and the new Huygens module for calculating the theoretical SPIM point-spread-function. Courtesy of Dr. Marc Duque Ramirez and Dr. Ritsuya Niwayama (Hiiragi group) and Dr. Stefan Terjung (ALMF) from the EMBL Heidelberg, Germany.