Huygens Object Analyzer Compact

Object analysis for SMLM data with the Huygens Localizer

The Compact version of the Object Analyzer is available for use within the Huygens Localizer, the Huygens software package that is specifically designed for analyzing single molecule localization data.

With the Object Analyzer Compact, you can gather statistics for individual objects or all objects within a single-channel image with the press of a single button. Objects are segmented by an effective seed-threshold level, and connection technique. Other available segmentation modes are the watershed method and a possibility to import label images created by other segmentation software. To remove too small objects in an early stage from the analysis, a garbage level can be set below which objects are discarded. After that, detected objects are automatically labeled and sent to a continuous Iso Surface Ray Tracing renderer.

Predefined filters and interactive tools allow you to select and remove objects with specific properties before further analysis is performed. The analysis can also be confined to a limited region by selecting a 2D region and defining a region of interest (ROI) on the screen.

The segmented and labeled image is shown as a colored iso-surface image. Each object is assigned a different color. Because the iso-surface renderer can recompute a new iso surface rapidly, the threshold can be changed interactively. By clicking on an object the user can read off the local statistics.

The following statistics are reported for each object, depending on the selected Experimental Preset:
  • Number and geometry of objects
  • Spatial location
  • Relation with neighbors
  • Relation with reference objects

Main features

  • Obtain both visual and quantitative information on all kinds of object analysis questions.
  • Automatically segment your data, or use custom settings for the segmentation.
  • Render a full analysis of your whole 3D/multi-channel image with a single mouse-click.
  • Count (intra)-nuclei or (intra)-cellular objects.
  • Perform Object Colocalization.
  • Measure intensities, distances, mass, diameter and more.
  • Intuitively select Anchors (reference objects) and Region Of Interest (ROI).
  • Measure nearness between objects in the same or in a different channel.
  • Refine your analysis with versatile object filter options.
  • Export your object statistics in all main spreadsheet formats.

How to use it

For a step-by-step explanation, we like to refer you to the:
For more information on object analysis and the Object Analyzer, we like to refer you to: