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Leica confocal TCS-SP2

This is part of the Backprojected Pinhole Calculator.

Details

The Leica TCS-SP2 has a system magnification of 3.6. However, the side length of its square pinhole is usually specified in Airy Disk units, referenced to a standard fixed WaveLength of 580 nm, making it independent of the actual overall magnification. It is dependent though on the Numerical Aperture of the objective.

Use the value reported by the microscope as an input in the form below.

Calculator

Number of Airy disks
Lens numerical aperture