Leica confocal TCS-SP5

This is part of the Backprojected Pinhole Calculator.

Details

The Leica TCS-SP5 has a system magnification of 3.0 (as reported by Leica). However, the side length of its square pinhole is usually reported in Airy Disk units, referenced to a standard WaveLength of 580 nm, making it independent of the actual overall magnification. It is dependent though on the Numerical Aperture of the objective.

Use the value reported by the microscope as an input in the form below.

Calculator

Use this form if you have the pinhole described in terms of Airy disk units:

Number of Airy disks
Lens numerical aperture

If you have the pinhole described by its physical side in SI units, use this other form:

Pinhole side (microns)
Objective magnification