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Leica confocal TCS-SP8


This is part of the Backprojected Pinhole Calculator.

Details


The Leica TCS-SP8 has a system magnification of 3.0 (as reported by Leica). However, the side length of its square pinhole is usually reported in Airy Disk units, referenced to a standard WaveLength of 580 nm, making it independent of the actual overall magnification. It is dependent though on the Numerical Aperture of the objective.

Use the value reported by the microscope as an input in the form below.

Calculator


Use this form if you have the pinhole described in terms of Airy disk units:

Number of Airy disks
Lens numerical aperture



If you have the pinhole described by its physical side in SI units, use this other form:



Pinhole side (microns)
Objective magnification


Contact Information

Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands


Phone: +31 (0)35 64216 26
Fax: +31 (0)35 683 7971
E-mail: info at svi.nl

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