Huygens Localizer: Interactive & Fast Localization Microscopy processing

The Huygens Localizer is a stand-alone software package for fast & high performance processing of Single Molecule Localization Microscopy data (SMLM), such as Photoactivated Localization Microscopy (PALM), Stochastic Optical Reconstruction Microscopy (STORM), Ground State Depletion (GSD), and DNA PAINT images. The Huygens Localizer is optimally designed for CPU and GPU computation, enabling a real-time processing experience. Main functionalities include:

Wizard-driven interface Interactive pre- and post-processing Various fitting methods Optional drift correction Works both on CPU and GPU: highly parallelized performance Visualization: interactive real-time rendering of localizations Exporting localization data & rendered image Interactive Object Analysis (Optional) Cluster Analysis (Optional)

Interactive pre-processing

Visualize the intensity flux over time, and crop useful region(s) from the total time-series. The Huygens Localizer is able to accurately estimate useful values from your image data, such as the photon-per-unit (ppu) value and the background. It can also estimate the PSF size from the microscopic parameters, which helps in more accurate localization results. In the particle detection stage, it is possible to interactively change settings such as threshold value to find optimal particle detection settings for your data.

Visualizing intensity flux (bleaching curve) Interactive cropping in time Interactive thresholding Powerful background estimation

Multiple CPU & GPU accelerated fitting methods

For optimal fitting, it is possible to choose from various fitting methods. All fitting methods are fully multi-threaded, and can run both on CPU-only as well as on an Nvidia GPU. The processing can be split up in block-sizes that are optimized for the (available) GPU RAM. This allows for efficient processing of tens of thousands of frames per minute. Least-squares Fit Weighted Least-squares Fit Maximum Likelihood Center of mass

Interactive Post-processing, Rendering & Analysis

Interactively render the localized data into a super-resolution image. Change the pixel (binning) size, and choose between various (weighted) rendering settings: bin in nearest pixel, fixed Gauss, weighted Gauss, width based on uncertainty. The automatic and accurate drift correction (no fiducial markers needed!) helps to automatically remove any thermal drift for the most accurate results. With multi-channel SMLM, a shift between the channels is typically present. The Huygens Chromatic Aberration Corrector can correct for this Aberration, resulting in more reliable multi-channel image analysis. With the interactive Object Analyzer (optional), it is possible to do object based statistic analysis. Measure for example: object count, distances, volume, surface, or overlap between objects in various channels.

Interactive Rendering & filtering of localization data Fixed Gauss, Weighted Gauss Automatic Drift Correction Chromatic Aberration Correction (optional) Interactive Object Analysis (optional) Cluster Analysis

Download now: get started!

You are always welcome to try out the Huygens Localizer on your microscope images. When you install the Huygens Suite on your computer, also feel free to try out our other products. You can also request pricing information or contact us for more information about the options best suited for your hardware and image processing needs.