SPIM/Light Sheet Fusion & Deconvolution WizardThe Fusion & Deconvolution Wizard for SPIM/LightSheet images is available in Huygens Essential and Huygens Professional since version 17.04. This Wizard which can be found under 'Deconvolution' in the Huygens taskbar.
Use it to deconvolve and fuse multiple views from different directions of the same specimen into one single superior image. By integrating the deconvolution into the fusion process, improved results can be obtained, while significantly simplifying and optimizing the post-processing steps.
ExamplesBelow you can find two examples of images that have been deconvolved and fused with the Huygens software in the Deconvolution & Fusion Wizard.
Denis Ressnikoff from the University Claude Bernard Lyon 1, in France was so kind to share with us his Zeiss Z1 data. His images were acquired at 360 degrees with 45 degrees steps. Deconvolution and fusion of this data by Huygens clearly improves the contrast of the images.
This second Fusion & Deconvolution Wizard example shows two images acquired separately with illumination from opposing sides with the Zeiss Z1. The images have been kindly provided by Shingo Nagawa from the Chinese academy of Sciences. Images show a leaf from the plant Arabidopsis deconvolved and fused with Huygens. A detail of the stomata and a line profile through these structures show a significant boost in resolution and signal to noise ratio.
Huygens Fused + Deconvolved