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HUYGENS Webinar invitation:


True Deconvolution & Restoration (10th March, 2020).

The essence of using Deconvolution is to reverse the Convolution process, which is how a microscope distorts images. Since convolution involves object blurring by the microscope type-specific point spread function (PSF), it is crucial that this PSF is accurately calculated or measured, and included in a deconvolution procedure with the best possible algorithms. These are currently MLE-based as implemented by Huygens. In this webinar, we will explain the image acquisition pitfalls and what true deconvolution includes. After this webinar, you have learned about the potential limitiations of other image enhancement techniques and know how to obtain the most reliable and accurate results from your valuable image data.

Automated, simple and fast deconvolution for ALL your microscopy data (April 7, 2020).

Huygens Deconvolution and Batch Express allows fully automated deconvolution of all your microscopy data. With just a few mouse clicks you have high quality results with Huygens. By simply defining a 'Watch Folder' in BatchExpress, you can let Huygens monitor and deconvolve all your images within this folder. Images can even be saved from a remote (microscope) workstation to this folder to obtain a deconvolved image 'on the fly'. Batch processing with deconvolution, chromatic aberration correction and image stabilization combined can also be scheduled for a specific time using templates in the Batch Processor, or by using the web-based user interface Huygens Remote Manager.

For an overview of all upcoming and past Huygens webinars, see this Webinar Schedule.

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Please note that the Webinar times are displayed in Central European Time (CET).
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170nm beads (TetraSpeck, ThermoFischer) were imaged with different widefield microscopes by Dr. Say-Tar Goh from CalTech. All the images were acquired at Nquist sampling. One of the raw datasets is shown here as a MIP projection (left panel). You can clearly see the background, noise, some hotpixels and blur. This images was deconvolved, and corrected for hotpixels and chromatic aberration using the Huygens Software (right panel).

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