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HUYGENS deconvolution for Abberior STEDYCON

Easy, compact and affordable STED imaging & Deconvolution for every lab!


The easiest, most compact and most affordable STED system available, now also includes an efficient connection to the Huygens Software. This new feature allows researchers get the most out of their high-quality STED images by using the powerful and renowned Huygens deconvolution MLE algorithms and image analysis modules in a stand-alone Huygens Professional package.

STEDYCON HUYGENS

Exchange data efficiently from STEDYCON to HUYGENS and optimize your image acquisition-processing worflow, and deconvolve/analyze your images with ease.

Super fast Processing

With full GPU acceleration support, the HUYGENS deconvolution for STEDYCON provides fast high-quality results, while the Deconvolution Express provides a fully automated deconvolution workflow.

Reliable MLE deconvolution

Includes a stand-alone HUYGENS Pro package; restoration is powered by the reliable and high-quality Huygens MLE deconvolution algorithms.



Raw Confocal
STED + Huygens Deconvolved
Z-stack MIP projection showing nuclear pore complex protein (red, Abberior STAR RED) and peroxisomes (cyan, Alexa 594) in mammalian cells. Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens.


About the STEDYCON: revolutionizing STED nanoscopy

The revolutionary STEDYCON is developed by the inventors of STED at Abberior Instruments to bring state-of-the-art STED nanoscopy (<40 nm resolution) to labs and facilities for a very affordable price. The STEDYCON is a compact add-on module that can be integraded with most modern fluorescence microscopes via a C-mount connection, effectively upgrading any conventional epifluorescence or confocal microscope into a STED nanoscope system in a matter of minutes.
The STEDYCON can image 4 separate confocal channels (405nm, 488nm, 561nm, 640nm) and uses a single STED (775nm) depletion profile, enabling intrinsic multi-channel STED alignment. Because of the unique and patented easySTED design, the STEDYCON is just as powerful as more complex models, yet much more compact and highly affordable. The unique design optimizes optical alignment and makes the system practically maintenance and service free.



Why HUYGENS?: get more from your data!

STED is an intrinsic super-resolution technique (i.e. no post-processing is required to obtain super-resolution), and can easily reach a resolution below 40 nm. However, even with highly sensitive photon-counting detectors, the physics behind STED imaging and the intrinsic photon shot-noise limit the photon counts per pixel, leading to noisier images compared to the confocal counter-part. This can be partially solved by increasing line-accumulation, i.e. scanning the same lines multiple times, but this has the downside to slow down the image acquisition, and tends to result in more photo-bleaching issues and may cause cell or sample damage. And this is where Huygens will help:

The Huygens Professional package offered with STEDYCON (see below for more details), helps researchers to get more from their confocal and super-resolution STED images, even with low signal and with noisy images. The Huygens CMLE and GMLE deconvolution algorithms have shown to perform extremely well on dim and very noisy STED data, enabling users to image much faster and with less line-accumulation. In turn this allows users to acquire images much faster, reduce bleaching and minimize cell damage. The powerful automatic and/or manual restoration options in Huygens make the images more reliable and easier to visualize and analyze, enabling researchers to save a significant amount of time with their imaging and image processing workflow.



Improve image acquisition speed

Increase contrast

Remove noise

Reduce background signal

Reduce blur

Increase resolution (lateral and axial!)

Drift correction (stabilization)

Spherical Aberration Correction

Advanced 2D & 3D Visualization & Analysis


Connection to HUYGENS Professional: optimized workflow

Huygens now fully supports the STEDYCON OBF data format. Users can open OBF data directly in Huygens via drag-and-drop or via the regular open command. Huygens automatically reads in all meta-data required for accurate deconvolution and further processing.*
The STEDYCON acquisition software also introduces a new feature that allows users to send any image from their imaging session to Huygens Professional with a single mouse-click. The dataset can be easily deconvolved in Huygens via the fully automated Deconvolution Express, or with the Deconvolution Wizard when the users prefers full manual control on the deconvolution settings.

* For optimal support of OBF meta-data in Huygens, the data should be acquired with version 4.0 of the STEDYCON software, and Huygens version 19.04 or higher is recommended. A license flag (I) is required in the Huygens license in order to make use of the Abberior Image Feeder functionality. Contact us if you require a test license.



Image

Confocal Raw
STED + Huygens deconvolved
Hela cells with actin cytoskeleton labelled (Phalloidin, Abberior STAR Red). Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.





Speed up: GPU acceleration

Both confocal and STED images acquired with STEDYCON can be deconvolved very efficiently on the CPU, but the processing can be further accelerated using a GPU card. For an optimal experience with any Huygens Deconvolution method we do advise using the GPU acceleration to give you high-quality results up to 30 times faster compared to CPU processing, resulting in near-instant deconvolution results. Click on 'Compare speed' to get an impression of the deconvolution speed with GPU acceleration vs CPU-only for a typical sized STED z-stack using a high-end GPU. For more info, visit the Huygens GPU page.



GPU accelerated

CPU-only

Learn more

HUYGENS for STEDYCON: what's included?

Abberior Instruments offers various Huygens Professional configurations, optimized for the STEDYCON and tailored to fit your various image analysis needs. A powerful, yet very affordable and compact GPU acceleration workstation can be optionally added to your STEDYCON configuration for most efficient image processing. The workstation specs have been selected by SVI and Abberior Instruments specialists to ensure an optimal supported solution.
The HUYGENS for STEDYCON configurations include a stand-alone Huygens Professional license and includes many useful functionalities such as: Deconvolution Wizard, Deconvolution Express, Batch Processor and various easy-to-use 2D and 3D visualization tools. The table below provides an overview of the various Huygens packeges offered by Abberior Instruments.

It is always possible to extend your Huygens license with additional options. Any Huygens STEDYCON package can be installed on the acquisition system, but it is also possible to install/migrate the Huygens license to a separate workstation. If you are interested in any other option not shown in the table below, please Contact us for the posibilties.
If you would like to learn more about which Huygens solution suits your research needs best, please contact either Abberior Instruments or SVI.
Contact SVI for multi-user and/or floating license possibilities, or request pricing information for a (multi-user) solution that best suits your needs.

Download Huygens now!
Primary hippocampal neurons with cytoskeleton proteins labelled (magenta, alpha-Adducin, Abberior STAR 635P and green, ßII spectrin, Alexa 594). Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.

HUYGENS for STEDYCON as offered by Abberior Instruments. Contact sales@abberior-instruments.com for pricing info.
HUYGENS Pro Starter Package for STEDYCON HUYGENS Pro Plus Package for STEDYCON HUYGENS Pro Plus Analysis Package for STEDYCON
Huygens Professional included as stand-alone package.
Includes: Deconvolution Wizard, Batch Processor, Operations Window, Scripting & many useful visualization tools.
Deconvolution Express & Batch Express
Confocal Optical option
STED Optical option
Object Stabilizer option
Visualization Tools
Time Option
Full File Reader
Small GPU option
Large GPU option (Optional)
Abberior Image Feeder option
1.5 hour Remote training by SVI staff
1 Year Maintenance & Upgrades
PSF distiller
Surface Renderer
Movie Maker
Colocalization Analyzer
(Wizard + Advanced + Compact)
Object Analyzer
Extended Maintenance & Upgrades (3 years total) (Optional) (Optional) (Optional)
Options marked with a can be optionally purchased via Abberior Instruments or via SVI.
Options marked with a and any other option in Huygens can be optionally added via SVI. Contact SVI for all the possbilities or request a quote.

Download now: get started!

You are always welcome to try out Huygens deconvolution on your confocal and STED images. When you install the Huygens Suite on your computer, also feel free to try out our other products. You can also request pricing information or contact us for more information about the options best suited for your hardware and image processing needs.

Download Now!



STEDYCON + HUYGENS deconvolution Image Gallery

Neurons Animated Small
Primary hippocampal neurons with cytoskeleton proteins labelled (magenta, alpha-Adducin, Abberior STAR 635P and green, ßII spectrin, Alexa 594). Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.


Raw Confocal
STED + Huygens Deconvolved
Hela cells with actin cytoskeleton labelled (Phalloidin, Abberior STAR Red). Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.


Raw Confocal
STED + Huygens Deconvolved
Hela cells with actin cytoskeleton labelled (Phalloidin, Abberior STAR Red). Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.


Raw Confocal
STED + Huygens Deconvolved
Hela cells with actin cytoskeleton labelled (Phalloidin, Abberior STAR Red). Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.


Raw Confocal
STED + Huygens Deconvolved
Hela cells with actin cytoskeleton labelled (yellow, Phalloidin, Abberior STAR Red) and Mitochondria labelled (cyan). Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.


Raw Confocal
STED + Huygens Deconvolved
STED140ROR nanorulers (GATTAquant). 70 nm distance between red and white sites. Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.


Raw Confocal
STED + Huygens Deconvolved
Centrosome linker. U2OS cells in which the centrosome-linker-protein rootletin was immunolabelled using secondary antibodies coupled to Abberior STAR RED. Sample was prepared by R. Vlijm at MPI for Medical Research, Heidelberg, Germany. Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.






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Contact Information

Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands


Phone: +31 (0)35 64216 26
Fax: +31 (0)35 683 7971
E-mail: info at svi.nl

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