The Huygens MIP rendererThe Maximum Intensity Projection (MIP) Renderer enables the possibility to obtain an orthogonal projection of 3D data from any given viewpoint.
PrincipleThe MIP renderer projects the VoXels on the screen by tracing near-parallel rays from a viewpoint far away through the data volume onto the screen image. Along each ray the maximum intensity encountered is written to the screen image. As a consequence, two MIP renderings from opposite viewpoints are mirror symmetric.
Animated MIP rendering of an isolated Rat Hepatocyte couplet recorded by Dr. Permsin Marbet at the Department of Anatomy, University of Basel, Switzerland, in the lab of Prof. Lukas Landmann.
UsageThe MIP renderer is highly customizable, giving the user the possibility to determine many parameters that play a role in the scene.
Apart from the viewpoint settings (tilt, twist, and zoom) the following options can be edited by the user:
- Time frame: a selection of the time frame (for time series).
- Channel parameters:
- Soft threshold: a limit of the fluorescent material that is visible.
- Color mode: a color selection for the material represented by each channel.
- First movie scene: the viewpoint of the first scene from which to make a movie.
- Last movie scene: the viewpoint of the last scene from which to make a movie. The movie will be automatically recorded interpolating positions between the 'First scene' and the 'Last scene'.
- Movie frames: the number of frames that will be included in the movie.
- Movie frame rate: the number of frames per second of the movie. Along with the 'Movie frames' it will determine the movie length.
- Render size: the size (in pixels) of the MIP scene.
- Render mode: whether the scene will be rendered in fast mode, high quality mode, or not rendered. It also includes the animation button to render a movie.
Rendering a movieThe Huygens Movie Maker allows to easily create sophisticated animations of multi-channel 3D images using the MIP Renderer and the other Huygens Visualization renderers.
Without the Movie Maker the MIP Renderer has the option to make simple animations, changing the MIP view point of the movie frames. In the MIP renderer, set viewpoint coordinates for the first frame, then switch to the Movie tab and click on the set 'First scene' button. Set now viewpoint coordinates for the last frame, and click on the set 'Last scene' button. To review the selected scenes click on the show 'First scene', 'Last scene' buttons. Select all the movie parameters, including the total number of rendered frames for the movie (Movie tab > Set number of movie frames). Finally, click on the Animate button, and select a destination directory for the movie.
Notice that all the time frames of Time Series images can be selected for animations.
See Make Animations for more details.