The Huygens Stitcher wizard, available for Huygens Essential and Huygens Professional, offers a unique easy-to-use option for combined high-quality stitching with deconvolution, and automated or manual vignetting correction.
Automatic Vignetting correction and Stitching
No Vignetting Correction
Auto Vignetting correction
Huygens stitched tiles of widefield (Leica) fluorescent Leica LIF data. The same data is shown with and without Huygens automatic vignetting and shading correction. Image represents a developing mouse cortex (P30) stained for Tbr1 and reelin.
Stitching of a Zeiss CZI with more than 1,700 tiles
An impressive 1700+ tile compound merged by the Huygens Stitcher to a large 60GB dataset. Notice the scaling bar indicating 2 mm. Raw data by © Carl Zeiss Microscopy GmbH.
- Mosaic preview of the tile content.
- Optimal positioning of each tile to guarantee smooth transitions across regions of overlap.
- Tiles with 0% overlap can still be stitched.
- Registration and deconvolution is supported by GPU .
- Support for 2D, 3D, multichannel, and time series images.
- 3D position optimization in X, Y and Z.
- No limit to the number of tiles that can be stitched.
- No limit to the total file size of the input sequence.
- Stitches all file types supported in Huygens (some by providing an XML file with layout information).
- Reads layout information from LIF and CZI formats automatically.
- Improved support for custom stitching patterns.
- Automatic vignetting estimation and correction for each channel.
- Manual vignetting and shading correction option by including recorded flat-field and dark-frame images.
- Integrated Huygens deconvolution for the merging of restored tiles.
The Stitcher wizard at work
The Stitcher can be started in Huygens Essential and Professional via the Deconvolution menu. If Leica LIF or Zeiss CZI images containing layout information are opened, the tile content will be automatically recognized and opened in the Stitcher Wizard. All other file types supported in Huygens can be stitched by providing a XML file with layout information.
Upon file opening, the wizard user interface will show the tile order. If needed, the acquisition pattern, start, percentage of overlap, and number of rows can be adjusted. Tile content can be previewed, and tiles can be selected/unselected for stitching. Automated and manual correction options are present to address any possible vignetting and shading issues. When all set, the Huygens stitcher will correct the tiles for shading effects and optimize the tile layout by analyzing the overlapping regions.
The Huygens stitcher wizard offers the unique possibility to automatically deconvolve each tile before the actual stitching is applied. For this purpose, a Parameter and Deconvolution template can be simply loaded within the wizard. By combining deconvolution, stitching, and shading correction in one single workflow, the Huygens Stitcher is able to make optimal use of hardware resources that are available.
For more information on the use of the Huygens Stitcher, we like to refer you to the Essential and Professional Manuals and to the Huygens Stitcher Tutorial Video.