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Winners of the Huygens Image Contest 2017


Many images and movies of high quality have been submitted for this year's Huygens Image Contest which made our job to select winners not an easy one. Similar to last year, we selected a prize winner for each of the two categories, i.e., "Cells" and "Tissues and Organisms".
We like to thank all the participants for sharing their images with us.

After meticulously reviewing the images, we decided to award the two first prizes to Dr. Romain Guiet from the BioImaging & Optics Platform at the Ecole Polytechnique Federale de Lausanne (Switzerland) for the category "Cells", and Dr. Dimitris Kapsokalyvas from the Department of Molecular Cell Biology, Maastricht University, The Netherlands, for "Tissues and Organisms".
Dr. Guiet submitted a Huygens deconvolved Zeiss LSM710 confocal image of excellent quality that is Christmas card ready material. The image represents a HeLa cell stained for DNA, F-actin, mitochondria, and tubulin. The image is displayed below and will cover our 2017 Christmas card.
The image from Dr. Kapsokalyvas also stands out, and nicely shows the improvement after Huygens deconvolution which is emphasized with the Huygens SFP renderer. We congratulate both with winning an ASUS Chromebook.
All other participants will receive a Google Chromecast (Audio or Video).


1st Prize: Dr. Romain Guiet, BioImaging & Optics Platform – EPFL, Lausanne, Switzerland
1st Prize: Dr. Dimitris Kapsokalyvas, Department of Molecular Cell Biology, Maastricht University,The Netherlands

3rd Prize: Dr. Tagide DeCarvalho, Department of Biological Sciencess, University of Maryland, Baltimore County, USA.
4th Prize: Dr. Gokhan Yilmaz, Department of Pharmacology, University of Oxford, UK.
5th Prize: Dr. Motosuke Tsutsumi, Nikon Imaging Center at Hokkaido University, Japan

A big thanks to the participants for making this Huygens Image Contest into a great success.
We wish you all nice holidays and a happy 2018, and hope you will join the next years Huygens Image Contest (again).

The SVI team


1st Prize Winner

Image from Romain Guiet

Hela cell stained for DNA (Azure), F-Actin (Spring Green), Mitochondria (Amber) and Tubulin (Bright Pink). Confocal images were taken with a Zeiss LSM710 microscope and deconvolved with Huygens software.
Hela cell stained for DNA (Azure), F-Actin (Spring Green), Mitochondria (Amber) and Tubulin (Bright Pink). Confocal images were taken with a Zeiss LSM710 microscope and deconvolved with Huygens software.


1st Prize Winner

Image submitted by Dimitris Kapsokalyvas

SHG signal of the rat soleous muscle's myosin imaged with a Leica two-photon microscope (NA: 1, 20x). Stack has been deconvolved with Huygens using a measured PSF, and visualized with the Huygens SFP Renderer. High quality imaging together with Huygens allows you to see through the muscle's A bands from one side to the other. The stack dimensions is 105x105x87 μm.
SHG signal of the rat soleous muscle's myosin imaged with a Leica two-photon microscope (NA: 1, 20x). Stack has been deconvolved with Huygens using a measured PSF, and visualized with the Huygens SFP Renderer. High quality imaging together with Huygens allows you to see through the muscle's A bands from one side to the other. The stack dimensions is 105x105x87 μm.


3rd Prize

Image in the category "Tissue and Organisms", from Tagide DeCarvalho

This image of a cyanobacteria mat/biofilm, which is stained with acridine orange, shows much color that is mostly due to autofluorescence. This aesthetic image was acquired on a Leica confocal, and deconvolved and MIP rendered with Huygens. It could well have been a product of modern art.
This image of a cyanobacteria mat/biofilm, which is stained with acridine orange, shows much color that is mostly due to autofluorescence. This aesthetic image was acquired on a Leica confocal, and deconvolved and MIP rendered with Huygens. It could well have been a product of modern art.


4th Prize

Image submitted by Gokhan Yilmaz

This colorful picture is a high-resolution image of Niemann-Pick Disease Type C (NPC) cells taken with a Leica STED using 100% 775 STED depletion. It shows after deconvolution with Huygens much detail and striking weak filopodia formation in the NPC cells.
This colorful picture is a high-resolution image of Niemann-Pick Disease Type C (NPC) cells taken with a Leica STED using 100% 775 STED depletion. It shows after deconvolution with Huygens much detail and striking weak filopodia formation in the NPC cells.


5th Prize

Movie submitted by Motosuke Tsutsumi



This movie was made with Huygens and shows fixed HeLa cells stained with Hoechst 33342 and alpha-tubulin. The 3D image was acquired with a Nikon A1 confocal (60x, NA 1.4) and deconvolved using Huygens Essential Deconvolution Express.

Copyright 1995-2018 Scientific Volume Imaging B.V. - Images and movies used with permission.