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The Huygens MIP renderer

The Maximum Intensity Projection (MIP) Renderer enables obtaining 3D to 2D projections of the highest intensities in an object. The result is similar to the MIP view in the Twin Slicer and Orthogonal Slicer tools, but in the MIP Renderer it can be obtained for any given viewpoint.

The MIP Renderer projects image voxels on the screen by tracing rays from a given viewpoint through the data.
For each ray the maximum encountered intensity is determined and displayed. To start the MIP Renderer, right-click on a thumbnail and select 3D Visualization → MIP Renderer from the pop-up menu, or choose Visualization → MIP Renderer from the main menu.

Starting from version 19.04, the MIP Renderer supports GPU acceleration. This greatly reduces render times. Note that GPU-based MIP renders can differ from CPU-based MIP renders. The most important difference is that GPU renders use a perspective projection (diverging rays, objects farther away are smaller), whereas CPU renders use an orthogonal projection (parallel rays, objects farther away stay the same size).

The MIP renderer showing an isolated Rat Hepatocyte couplet (recorded by Dr. Permsin Marbet at the Department of Anatomy, University of Basel, Switzerland, in the lab of Prof. Lukas Landmann)
The MIP renderer showing an isolated Rat Hepatocyte couplet (recorded by Dr. Permsin Marbet at the Department of Anatomy, University of Basel, Switzerland, in the lab of Prof. Lukas Landmann)


Usage

The MIP renderer is highly customizable, giving the user the ability to set many parameters that play a role in the scene. The following parameters can be set:
  • Twist, tilt, zoom & pan: Adjust the viewing angle and camera position.
  • Time frame: Select the time frame in a time series image.
  • Detector: Select the detector in a multi-detector image.
  • Channel parameters:
    • Soft threshold: Only voxels above a certain intensity are taken into account during rendering.
    • Color mode: A color selection for the material represented by each channel.
  • First movie scene: Set the render parameters for the first scene of the movie.
  • Last movie scene: Set the render parameters for the last scene of the movie.
  • Movie frames: The number of frames that will be included in the movie.
  • Movie frame rate: Set the framerate (fps) of the movie.
  • Render size: The size (in pixels) of the MIP rendering.
  • Render mode: Whether the scene will be rendered in fast mode, high quality mode, or not rendered. It also includes the animation button to render a movie.

Rendering a movie

The Huygens Movie Maker allows you to create sophisticated animations using the MIP, SFP and Surface renderers. Note that all the time frames of Time Series images can be selected for animations.


Animated MIP rendering of an isolated Rat Hepatocyte couplet recorded by Dr. Permsin Marbet at the Department of Anatomy, University of Basel, Switzerland, in the lab of Prof. Lukas Landmann.


However, the MIP renderer can also create simple animations on its own by transitioning between two custom keyframes.
Set the render parameters for the first frame and click the Movie tab, Set view → First scene. Now adjust the parameters for the final frame, and click SET view → Last scene. The frame count and frame rate can also be set in the Movie tab. Finally press the animate button to render the movie as an AVI or as a series of Tiff files.

The exported AVI files use the MJPEG codec and can be played by most video players.

See Make Animations for more details.