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Scientific Volume Imaging B.V.

Scientific Volume Imaging

Deconvolution - Visualization - Analysis


™Huygens Widefield Deconvolution Software

Boost signal, contrast and resolution with the most reliable algorithms available

RAW Widefield
Huygens Deconvolved
smFISH in Drosophila Muscle shows mRNA composition of megaRNPs (yellow and red). Nuclei are stained with antilamin antibody (blue). Image by Dr. Akiko Noma, Dr. Carlas Smith & Dr. David Grunwald, University of Massachusetts Medical School, USA.
One of the main issues with widefield imaging is out-of-focus blur, which typically hinders 3D imaging and clear object recognition and segmentation. Luckily, Huygens true deconvolution can use this fluorescence to increase image resolution and specific object signal, which can be more than 10-fold. These improvements, together with Huygens' well-known spherical aberration correction, offers true image detail, even deep within thick samples. Brightfield images also greatly benefit from Huygens Widefield & Brightfield Microscope deconvolution.
Noise reduction

Noise correction

Increased Resolution

Increased resolution

Reliable Quantification

Reliable quantification

Most advanced algorithms

Huygens uses Maximum Likelihood Estimation algorithms

All Microscope Brands

All Widefield & Brightfield Microscopes are supported.

Deep tissue imaging

Out of focus light is optimally corrected.

Testimonials

Our images are improved amazingly by the Huygens software, and we are very happy with it! Di Zi, MSc, PhD student at Leiden Institute of Advanced Computer Science, Leiden University, The Netherlands.
Huygens seems to surpass our other products at least for deconvolution and also surface rendering. Technical support is truly tremendous. Huong Ha, graduate student Neuroscience Program, Stanford University, USA.
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Original

3D widefield imaging

All fluorescence microscopy images suffer from out-of-focus light, which hinders clear 3D rendering of the object. This light is especially problematic when there is Spherical Aberration due to a mismatch between the refractive indices of the sample and the lens medium. The image quality then gets progressively worse as you move deeper within the sample. With Huygens, deconvolution is done in a depth-dependent manner such that the resulting image is consistent in quality over its entire depth range. A reliable 3D rendering can then be made and analyzed.

Click here for more info on the Huygens depth-dependent PSF.

Image description: SFP rendering before (left) and after (right) Huygens Deconvolution of a 39x51x10 μm Widefield image. Images were automatically and subjectively segmented using the Otsu algorithm. Data courtesy of Dr. Alexia Ferrand, Imaging Core Facility, Biozentrum, University of Basel
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Original

Bleaching correction

The Huygens Bleaching Corrector can correct for photobleaching and illumination instability in the depth and time dimension. The resulting image is consistent in quality over a larger depth or time range, as compared to the raw input image.

In addition, clear images can be retrieved from low-signal raw data since Huygens Deconvolution removes noise while improving contrast. Thus, lower light intensity can be used during imaging, allowing for deeper and longer imaging with less bleaching.

Click here for more information on bleaching correction.

Image description: MIP rendered widefield image of a cultured cell before (left) and after (right) using the Huygens Bleaching Corrector. Plots show the mean intensity I over the axial direction z.

Use in research

Dossin, F., Pinheiro, I., Żylicz, J.J. et al. SPEN integrates transcriptional and epigenetic control of X-inactivation. Widefield movies and images were deconvolved with Huygens Nature 578, 455–460 (2020).

A. Bello-Gamboa et al. Actin reorganization at the centrosomal area and the immune synapse regulates polarized secretory traffic of multivesicular bodies in T lymphocytes. Widefield and confocal images were deconvolved with Huygens. J Extracell Vesicles (2020) Jun 19;9(1):1759926.

For more, see Scientific Publications

The best deconvolution results can be achieved by using a measured PSF extracted with the Huygens PSF Distiller. After deconvolution, images can be used for reliable image analysis, for example colocalization analysis.

PSF Distiller Colocalization Analyzer

More information

Introduction to deconvolution Point Spread Function Huygens Deconvolution Restoration examples

Raw Widefield
Huygens Deconvolved
Stained structures (kinetochore complex) involved in cell division. Image kindly provided by Livio Kleij (facility) and Martijn Vromans. Center for Molecular Medicine, UMC Utrecht, The Netherlands. See more: Images in the field of Genetics Biology
Raw Widefield
Huygens Deconvolved
smFISH in Drosophila Muscle. Composition of megaRNPs: Pak, Par6, and Cask mRNAs. Nuclei are stained with antilamin antibody (blue). Published in: https://pubmed.ncbi.nlm.nih.gov/32158910/. Image kindly provided by Dr. Akiko Noma, Dr. Carlas Smith & Dr. David Grunwald RNA Therapeutic Institute (RTI), University of Massachusetts Medical School, USA. See more: Images in the field of Cell Biology