Scientific Volume Imaging

Huygens 软件更新消息

以下的更新资料，在没有特别注明下，泛指所有 Huygens 软件，有注明下 E = Huygens Essential, P = Huygens Professional, S = Huygens Scripting, C = Huygens Core, T = Huygens Titan。如果您想寻找旧版的更新资讯，可查看 WhatsNewArchive：

惠更斯 2017 年春季版 17.04 已经推出了( 2017 年 5 月 ) !

摘要

Light Sheet/ SPIM Fusion Wizard 光片照片的 Fusion 与去卷积 Wizard 功能，这个功能对 MultipleView SPIM 的数据；或激发光片固定在同位置，但样本以不同角度旋转采集得的图像均可以使用。惠更斯 Huygens 会把原始数据作去卷积处理再以 Fusion 组合数据，光片照明的散射亦同时得到校正。

支持多张显卡 GPU 的批量处理 Batch Processor 加速

批量处理 Batch Processor 加入更多对 Object Stabilizer 的选项，自由度更大，更弹性。

支持高清 4K 屏幕

惠更斯的介面提供亮度低的暗色皮肤作选择，黑暗的实验室采图与图像处理时，提供对视觉较舒适的介面，当然惠更斯原来的皮肤颜色仍是选项之一。

光漂白校正 Bleaching Corrector，对 XYZ , XYT , XYZT 作用测量与校正。

支持以下新文件格式。 - Leica LOF - Metamorph ND version 1.0 and 2 - Andor .Tiff

改善以下文件读取。 - Zeiss CZI - Leica LIF - Olympus VSI - Nikon ND2 - OME Tiff

Minor changes

• (All) CZI reader:
  • Support for JPEG-XR compression.
• (All) ICS reader/writer:
  • Support for parameter states.
  • Support for SPIM parameters.
  • SVI now maintains the open source libics library for reading and writing of ICS data. Since Huygens uses libics the library will be released in sync with Huygens from now on.
• (All) TIFF reader:
  • Support for 32-bit float files.
• (All) LOF/XLIF readers:
  • Support for DSL data.
• (All) OME-TIFF reader:
  • Metadata is loaded.
• (All) GPU support:
  • Made switching to a different GPU device more robust
• (E/P) Huygens - LAS X:
  • Faster communication and data transfers.
• (E/P) Stitcher:
  • Improved support for custom stitching patterns.
  • More visualization controls.
  • A new MIP visualization mode.
  • Better performance and less memory consumption.
  • Disable background estimation and bleaching correction for the tile deconvolution.
• (E/P) Batch Processor:
  • Improved GUI.
  • Support for loading LIF files with multiple levels of sub-images.
• (E/P) Improved icons.
• (P) Improved image convert window.
• (E/P) Crosstalk corrector:
  • Reduce noise option
  • Correction is now energy conservative.
• (E/P) Tab completion is available in the open file window on Linux.
• (C) SFP renderer:
  • Threshold data by Otsu's method for rendering raw and deconvolved images.

Bug fixes

• (All) CZI reader:
  • Fix 'Scale if necessary'.
• (All) LIF reader:
  • Several fixes.
• (All) ND2 reader:
  • Several fixes.
• (All) Background estimation:
  • Fix background calculation on the CPU for floating-point images containing integer values.
• (E/P) Drag & Drop:
  • Fix Windows and Linux distributions.
• (E/P) Communication with Leica's LAS X software:
  • Fix floating licenses.
• (E/P) Histograms:
  • Fix potential bug in the calculation of 5+ channel histograms.

Huygens 16.10 新版发布 (2016 年 10 月 )

摘要

Huygens Professional / Essential

快速去卷积捷运 Deconvolution Express 只需一个按键 Deconvolve 就自动快速处理，快速处理的条件将由惠更斯 Huygens 作自动估计，测量，图像的讯噪比与所需数据 metadate 会由惠更斯 Huygens 以图像的讯号与结构作出智能评估再直接进行去卷积处理，方便快捷但以结果准确计算作优先条件!

GPU 显卡将支持更多的惠更斯 Huygens功能，如

• 背景估计，
• 使去卷积速度更快速
• 支持 Object stabilizer
• 色差校正支 Chromatic Aberration Corrector
• 光片照明 SPIM

加入了新的光片模式 如: Multi View 'MuVi', Bessel beam, two-photon Bessel beam

Huygens server 功能支持徕卡 LAS X 数据交换功能，把数据交换提升到与电脑之间的交换 !

Minor changes

• (All) Background estimation:
  • Improved pre-filtering of the image when calculating the "WF" mode.
• (All) GMLE deconvolution:
  • improved handling of very shallow or 2D images.
• (All) Windows installer:
  • Warn the user if Huygens is running when there's a request for uninstalling or reinstalling the software.
• (All) GUI:
  • Default thumbnails size is now chosen according to the monitor size.
  • The numerical keypad's "enter" key behaves in the same way as the main keyboard's "enter"/"return" key on Linux and Mac.
  • The "enter" key invokes the selected option instead of the default option in questions with multiple answers.
• (All) CZI reader:
  • Add support for cases where the ZISRAWDIRECTORY segment is missing.
  • Fix case where the pinhole of one of the channels is missing.
  • Update internal magnification of LSM800.
• (E/P) Colocalization Analyzer:
  • Extra column in the results tab to show the contribution of each object to the total coloc coefficient.
  • Background value can be set to 0.
  • Improved return value handling in Tcl coloc command.
• (E/P) Stitcher:
  • Improve support for custom stitching patterns.
  • Allow 0% tile overlap.
• (E/P) Communication with Leica's LAS X software:
  • Show 3 decimal digits at most in scaling factors.
  • Add XML headers to Huygens attachments.
• (E/P) Object Analyzer:
  • Improved performance.
• (E/P) Track Analyzer:
  • More intuitive names, labels and help texts.
• (E/P) GPU Window:
  • Added the possibility of changing GPU card.
  • GPU status is now linked to on/off preference without waiting.
• (E/P) Twin Slicer:
  • Frame number and time (seconds) are now shown by default on time series.

Bug fixes

(E/P) Communication with Leica's LAS X software: Opening a LIF sub image in Huygens and exporting to Leica results now in more precise meta data. Restore the use of full 16-bits range when exporting to Leica which was briefly disabled in the 16.05.1X version. (E/P) Batch Processor: Fix slow behaviour when listing many files or deconvolution jobs. (E/P) SFP Renderer: Fix several scene inconsistencies. Fix a crash on an image with very many pixels in a single slice. Fix the re-centering of the image after the windows is resized. (E/P) MIP Renderer: Fix the re-centering of the image after the windows is resized. (E/P) File Series tool: Fix launching the Stitcher with only 2 tiles. (E/P) Stitcher: Fix displaying only 1 channel when opening file series. Fix hidden brightness tool with multi channel images. (E/P) Fix potential issue on opening an internet browser from hypertext in Huygens. (All) Histogram: Fix marginal case where the count can be off by 1.

Huygens 16.05 新版发布 (2016 年 5 月 )

摘要

Huygens Professional / Essential

• 拼图 (Stitcher)：
  • Essential 也能运作行拼图功能及暗角校正功能
  • 显卡 GPU 支持拼图运作
  • 对于 *.tiff 系列拼图的支持，用户只要知道采图顺序 (由上到下，左至右，以及其他序列)，可以自由的选择采图顺序作出拼接
• 光片照明 (SPIM) ：
  • 新的激发光片模式更能配合采图时的设定
  • 全面支持蔡司光片照明文件
• 截图功能 (Crop)：
  • 针对大型数据，提高了运行效率与速度
• 可视化 (Twin slicer)：
  • 加入 MIP 与 SUM 投影模式
  • 对比 Gamma ，用户可以自由调整
• 显卡GPU功能：
  • 对于问题出视时，提供更准确的解决方案视窗
• CPU核的使用率提高了50%
• Professional 加入全新的可加载模块

Minor changes

• (All) In Tiff reader:
  • Add support for JPEG compression.
• (All) In LSM reader:
  • Add LSM 800.
• (All) In CZI reader:
  • Better handling of multiphoton parameters.
• (All) in VSI reader:
  • Update to latest file specification.
• (All) In Imaris writer:
  • Improved handling of single-slice 3D images.
• (All) In LIF reader:
  • Extract and save the sub image meta data from a LIF collection.
• (All) On Windows, add support for NUMA processors including all their cores.
• (All) Workaround for NVIDIA's issue where no processing of specific image geometries is accepted at all by the GPU card.
• (E/P) Surface Renderer:
  • Add the same render buttons as in the MIP and SFP renderers.
• (E/P) Chromatic Aberration Corrector:
  • Better performance and more accurate scale and rotation.
• (E/P) Othogonal Slicer:
  • Remove deprecated version. Enable newest version only.
• (E/P) MIP renderer and SFP volume renderer
  • Changed the channel selector which enables to switch off channels.
• (E/P) Deconvolution Wizard:
  • Fix hard error on clicking the 'Cancel' button during deconvolution.
  • Add 'GMLE' algorithm choice to confocal data sets. Skip it on 2D data.
• (E/P) PSF distiller:
  • Support for SPIM/light-sheet microscopes.
  • (E/P) Object Analyzer:
  • Speed enhancements when analyzing large number of objects.
• (E/P) Object Stabilizer and Object Tracker:
  • Fix stage-dependent 3D slicing.
• (E/P) Significant speed enhancement for the median filter on byte and integer image types.
• (E/P) Improved error reporting of GPU-related problems.
• (P) Performance Wizard:
  • Limit number of trial threads to the CPU. Skip the GPU where all threads are used.
  • Try the licensed microscopy types only.
• (E) Add Leica LASX export button.
• (C) Add on-demand GPU option to deconvolution templates.

Bug fixes

• (All) In ICS reader:
  • On Windows: reading and writing data sets that are larger than 2GB.
  • On Windows: allow the use of extended characters (such as é) in file and directory names.
  • Several fixes for ICS related crashes and for failures to read/write ICS files.
• (All) In LIF reader:
  • Fix for reading data generated by Leica´s Live Data mode module.
• (All) In ND2 reader:
  • Fix hard error on specific data.
  • Fix potential memory corruption.
• (All) In Ome writer:
  • Large images are written correctly.
• (All) MultiMIP yields more consistent results when single-slice images are used.
• (All) Delayed event handling has been improved such that potential problems are prevented when Huygens is closed.
• (All) Fix regression in histogram command.
• (All) On Windows, fix potentially incorrect System IDs which encode 0 cores and lead to astray image processing.
• (E/P) In Batch Processor:
  • Fix interpretation of 'varPsf' option.
• (E/P) In GPU QMLE, fix display of deconvolved result.
• (E/P) In Plot Profile Tool:
  • Fix far from centered default profile.
• (E/P) In Plot Flux Tool:
  • Fix grey color palette.
• (E/P) In File Series Tool:
  • Fix error when clicking 'Load Selection' on just one file.
• (E/P) In Stitcher:
  • Fix zoom jumps.
• (E/P) In MIP renderer:
  • Fix incorrect verbosity level leading to multiple questions.
• (E/P) in Surface Renderer:
  • Fix rendering artifacts on extreme large zoom factors.
  • Fix default movie preset.
• (E/P) Fix incorrect busy state on opening an unsupported file.
• (E/P) Stabilizer: fix crash on very unstable images.
• (T) Indexer logs from Titan do not consume as much disk space anymore.

Huygens 15.10 新版发布 （2015年秋季）

摘要

• 显卡加速去卷积可用于所有的去卷积计算方法
• 光片照片功能的改善
• 徕卡文件读取速度增加，读取时的记忆体需求减低，新的数据也能读取

Huygens Professional & Essential

• 物件追踪功能 (Object Tracker) 及 Object Stabilzer 校正功能加入可视化功能 (Orthogonal Slicer)
• Mip 渲染功能
  • 支持复杂类图像
  • 支持更大的图像体积
  • 高清模式的可视化品质提高

Huygens Professional

• 拼图功能
  • 支持 tiff 及 ⅹml 格式
  • 新的 Flip & Swap 功能可改变小图的方向，使拼接更有弹性

Huygens Titan

• 主视窗更流线化
  • 用户第一次使用时的使用导航指示
  • Project 与 Annotaton 的区别更清晰
• 附加标签
  • Tⅰtan 的监察
  • 可移除的外置硬盘的状态
• 在超大数据读取时保持快速反应

Minor changes

• (All) In deconvolution algorithms:
  • Report bleaching factors per slice and per frame.
• (All) In LSM reader:
  • Add LSM 780.
• (All) In HDF5 reader/writer:
  • Add support for SPIM parameters.
• (All) In ICS reader/writer:
  • Add support for STED parameters.
  • Add support for channels with different microscope types.
• (All) in Compute Engine:
  • Faster opening of all large files.
  • Performance increase in stat command for byte images.
  • Large performance increase in range command, especially for byte images.
  • Performance increase in hist (histogram) command.
  • Performance increase in cs (copy and stretch) command.
  • Add support in label function for 2D binary images.
• (E/P) In Parameter Editor:
  • Add embedding media Vectashield and Mowiol.
• (E/P) In MIP Renderer:
  • Automatically renders when zoomed with the mouse wheel.
  • Higher resolution for the quick mode engaged while dragging.
• (E/P) In Surface Renderer:
  • More efficient memory usage.
  • Increased height to prevent occlusion of widgets in Movie Maker mode.
• (E/P) in Renderers:
  • Tilt can now be 360 degrees for all renderers.
• (All) Improved robustness of Floating License requests.
• (All) Improved reading of Z-stacks and T-stacks in ND2 files.

Bug fixes

• (All) in Compute Engine:
  • borderFill for 2D images would clear image.
• (E/P) In Chromatic Aberration Corrector:
  • Fix estimation of rotation between channels.
• (E/P) In Object Analyzer:
  • Seed and threshold manipulation with keyboard now also added to history.
• (E/P) In Colocalization Analyzer:
  • Don't allocate too much RAM when computing coefficients with a mask.
• (E/P) In SFP Renderer:
  • Fix potential overflow on the number of voxels.
  • (E/P) In MIP Renderer:
• Show the displayed frame correctly:
• (E/P) In Surface Renderer / Object Analyzer:
  • Fix display of binary images.
  • Fix potential artifacts in integer images when the selected threshold is also an integer.
• (E/P) In Object Analyzer:
  • Fix position of loaded ROI.
• (E/P) In PSF Distiller:
  • Fix potential empty result.
• (E/P) In Movie Maker:
  • Fix high quality rendering for the MIP and SFP renderers.
  • Fix the preview when the transformation includes a shift.
  • More precise zooming interpolation (fixed stuttering).
• (All) Fix several small memory leaks in file readers.

Huygens 15.05 的发布重点 (2015 年五月)

摘要

• 支持利用 GPU 加速进行 CMLE 及 TM 的去卷积计算 （请特别向 SVI 要求试用及更多资料）。

• 对 Light Sheet 系统 (SPIM) 的支持
  • SPIM PSF 的计算
  • 激发光片的厚度是较 PSF 大的话，Huygens 利用 brick layer 进行去卷积。

所有的 Huygens 产品

• 新的 reader 读取 VSI 文件挡
• 新的 reader 读取 JPEG 文件档
• 新的 reader 读取 Leica 的 LOF 及 XLIF 文件档
• 浮动 licenses

Huygens Essential 及 Professional

• 新的色差的修正功能 (Chromatic Aberration Corrector)
  • 取代旧的 Chromatic Shift Corrector
  • 新的 GUI 使流程畅顺
  • 新的色差修改模式修正通道之间转动的角度，及因缩放而产生的偏差
• Deconvol​​ution wizard
  • 流程的改善使 template 的执行更畅顺
  • 进入去卷积 wizard 时，wizard 为您提供图像的资讯
• Parameter Editor 的全新介面
  • 这个全新介面将出现于 Batch Processor, Distiller 及 Operation Winow
• 新的 API 让用户可使用 Huygens 与其他软件作数据交换（请联系我们）

Huygens Professional

• 拼图功能 stitching
  • 支持 Tiff 文件档
  • 新的暗角校正选项
  • 效率的提高
  • 更精准的拼图结果
• SPIM 的支持
• 在 Operation Window 中，使用 Template Editor 把去卷积 job 转换成 Template
• 延伸 Tikhonov-Miller 去卷积计算及自动发生理论值 PSF

Huygens Titan

• Freeware 模式容量的增加(由 10GB 增加到 200GB!)
• 支持 JPEG 在图像索引及寻找
• 新的寻找线索
  • Nyguist 采像频率好的图像
  • 针对需要作 Stabilization 及共定位的图像
  • 图像的分辨率
• 搜索功能的改善
  • 文件档名的搜索可以使用一名或多个关键字
• 观察 Titan 活动的改善
• 可设 directory 成 template 文件 (thumbnails/ database)

Minor changes

• (All) Improved error messages for img sub commands.
• (T) In Huygens Titan:
  • Removed redundant version check that slightly impacted indexing performance.
  • Improved error handling when a thumbnail could not be generated due to the memory limit. An 'image too big for now' message is now displayed instead of a generic error message.
  • Notify that an image is not yet indexed in the image metadata area instead of waiting.
• (E/P) In Deconvolution Wizard:
  • New welcome screen presents an overview of important optical parameters.
  • Removed unused iteration field for qmle algorithm.
  • Improved workflow for the template save stage.
  • Reduced processing time when executing templates.
  • Enabled 'estimate psf' button for STED Channels when using a theoretical PSF.
  • Added "Autocrop" to the template functionality.
• (E/P) In Gallery:
  • Border is now dependent on image dimensions instead of fixed.
  • Display a black border when #000000 is selected as border color.
• (E/P) In Template Editor:
  • Better layout (some widgets were obscured).
• (E/P) In Twin Slicer:
  • Reset view now also resets contrast stretch.
• (E/P) In Ortho Slicer:
  • Tool can now be closed via the File menu.

Bug fixes

• (All) In VSI reader:
  • Long file names could be truncated, preventing the files from being opened.
• (All) In ND2 reader:
  • Long file names could be truncated, preventing the files from being opened.
• (All) In CZI reader:
  • Huge CZI files could not be loaded in Windows.
• (All) In "img preOpen" command:
  • Resources could be leaked.
• (E/P) In Deconvolution Wizard:
  • Some templates would not load PSF.
  • PSF could only be loaded once.
• (E/P) When using templates the Twin Slicer could not be launched at the last wizard stage.
• (E/P) In Object Analyzer:
  • ROI could be incorrectly shifted on loading from file.
• (E/P) In Gallery:
  • Clicking on a slice would not show the slice in the detailed view.
• (E/P) In Colocalization Analyzer:
  • The analysis could be carried out on a padded image.
• (E/P) In Surface Renderer:
  • A crash could occur when the MIP and slicer were used from a specific viewpoint.
• (E/P) In PSF Distiller:
  • The wizard would get into an undefined state if a channel didn't contain any beads.
• (E/P) In Performance Wizard:
  • The tuning would not be aborted after closing the window.
  • The wizard could not be restarted after closing it from specific window controls.
  • A run would not get started without a confocal flag.
• (E/P) In Batch Processor:
  • File series where the 'h' of 'Ch' was missing would not be recognized.

Huygens 14.10 版的发布重点

• 新的工具： Gallery, 在桌面（Thumbnail overview ）上可随意以各角度浏览您的三维显微镜图像。
• 新的工具：Performance tuner，测量 Huygens 在您的电脑运作的效率，再调节 Huygens 使用几核去进行去卷积的计算。
• 新的功能：Image Feeder，这个功能相等于一个属于 Huygens 的 Dropbox 。
• 更新的工具： Hot Pixel Remover，分析您的显微镜图像的原始数据再对 hot pixel 作出分析及修正。

所有的 Huygens 产品

• CMLE 去卷积计算效率的提高，及加入新的快速计算模式。
• GMLE 去卷积计算模式，适合使用于噪音较大的图像，是特别为 STED 与共聚焦的图像而设计的。
• 去卷积报告，加入背景的修正；报告模式是以每一通道、每一格图像的形式展示。
• File Reader 的更新，读取新的 Olympus *.VSI 文件。

Huygens Titan

• 当 Titan 读取庞大的 *.LIF 档时，Titan 早在索引 (indexing) 的时候，把 *.LIF 文件结构中的各图像及资料读取。 *.LIF 文件中含多图像，Titan 也能分辨出来。
• 为了减少电脑处理器的负担并增加工作效率，当 Titan 遇上庞大的文件档，Titan 利用 placeholder 显示出 thumbnail 并处理其他的图像搜索，使 indexing 继续进行。
• Titan 搜索能力的加强，文件中的图像是以时间序列的图像或一文件中含有多图像的档案都可以分辨。

Huygens Professional

• Stitcher 拼图功能
  • 加入了暗角校正
  • 增加了工作效率及更有效的利用记忆体。
  • 能教正原始 *.LIF 档的每一块图像的方向。

Huygens Essential

• 加入了与 Leica LAS X 的互动，图像可从 Huygens 直接切换到 LAS AF X 或从 LAS AF X 切换回 Essential 。

有关 Huygens 软件的其他更新

(All) 支持所有的 OME-Tiff 文件档。 (All) subBrick 重新命名为 varPSF ，一名跟这个功能比软贴切的名称。 (All) 在 genPsfExpl 指令中，STED saturation factor 的预先设定值由 40 改成 30 。 (E/P) 在去卷积功能中 Deconvolution wizard thumbnails 除去 Save as HDF5 的选项。 (E/P) 在 Deconvolution wizard 中，不再使用 Twin slicer preference 而使用预设设定。 (P) 在 Stitcher wizard 拼图功能中，不再使用 Twin slicer preference 而采用预设设定。

Bug Fixes

(All) In license mechanism: string licenses with no extra flags did not get recognized. (All) In LSM file reader: Emission wavelength was not estimated accurately for low pass and short pass filters. (E/P/C) In Batch processor and HuCore jobs: the sampling distances were not properly loaded from the templates, instead the metadata values were used. (P/S/C) In ICS file writer: data compression was not enabled as intended. (E/P) In Batch Processor: the selected sub image did not get processed. Instead, a different sub image from the same data set was deconvolved. (E/P) In Stabilizer: a license check for Object Analyzer was not needed. (E/P) In Stabilizer: the outliers slider did not behave as intended. (E/P) In Colocalization Analyzer: Huygens could fail to launch the tool. (E/P) In SFP renderer: the scene's aspect ratio and perspective could be wrong depending on the image geometry. (E/P) In thumbnails: a license check meant for Tracker checked on a different option instead. (E/P) In PSF Distiller: an error could be thrown when closing the tool before reaching the last workflow stage. (P) In Stitcher: the normalization of the flat-field image could fail. (P) In Stitcher: the mosaic corners could look a bit too rounded. (T) Search on filename instead of image name in the advanced search. (T) Fixed bug where images with very large metadata fields where not loaded correctly. (T) Fixed bug where searching of path doesn't work in the advanced search. (T) Increased size of annotation comment field. (T) Fixed error where Titan would not go to the correct directory when select 'Find in library tree' in the advanced search tab.

Huygens 14.06 版的发布重点

• 全新 Huygens Titan 诞生了！
• Huygens Professional 中全新拼图 Stitcher 功能。
• 新去卷积计算模式：GMLE。
• 新的 sub-brick wise 去卷积。

所有 Huygens 产品

全新的方法去处理 PSF 形状的改变 点扩散函数 PSF 常常受到介质的改变而导致球面色差的影响，结果导致 PSF 形状的改变，这个问题常随着样本的深度而产生。之前的解决方法是把图像分割成小块，每一小块具备有本身的 PSF ，这是一个有效率的方法，可是也会做成失真的效果。新的方法叫 "sub-brick" 方法，把基于 Fast Fourier Transform (FFT) 卷积操作成为去卷积计算的核心换成多个 FFTs ，而每一个具备不同的 PSFs，这个新的计算方法可使用于 CMLE, QLME 及新的 GLME 去卷积模式中。

GLME 全名为 Good Roughness Likihood Estimation，这个模式是基于最大后验概率（Maximum a Posteriori probability) 来进行计算，这个模式正适合使用于 PSF 形状的改变。 GMLE 是处理STED 数据的预设模式，其他系统进行去卷积时需要到 Expert Mode 去设定 GMLE 模式。

全新的软件 Huygens Titan 的诞生

• 为您的显微图像作出索引。 Titan 会主动寻找、作出索引，用户无需上载图像，也不必作日常的维持工作，您的图像将保存在同一电脑中。因为 Titan 并不是资料库，Titan 主动寻找、索引的运作方式是独有的。
• 离线索引：Titan 除了能寻找及索引您电脑中的显微图像外，也能搜索外置硬碟、记忆棒。用户只需要把记忆棒或外置硬碟连接上，Titan 可读取、寻找及为您的显微图像作索引，用户使用 Titan 后，把记忆棒移除，图像保留在记忆捧中（除非用户特别的存档），否则不增加共用仪器电脑的负担。
• 您可以为您的图像加上文字记号或注解，这个记号只用在 Titan 中，并不改变您的文件的档案，而文件具备同一个记号可以整理成为一个项目 (Project)。
• 您可以利用您的显微图像中的资料 (metadata) 或附加的注解为搜查线索。
• 在 Lightbox 中快速的浏览寻找出的多维显微图像。
• 直接把结果输出到 Huygens Professional/ Essential。

(P) Stitching 拼图功能

• 全新的自动拼图功能。
• 含有去卷积功能。
• 暗角的修正。
• 目前支持 Zeiss *.CZI 文件档与 Leica 的 *.LIF 文件档。

(E/P/C) Batch Processor ​​批量去卷积及图像修复功能

• 加入新的 Autocrop 功能。

有关 Huygens 软件的其他更新

• (All) Support for two level nesting LIF files.
• (All) The 'preOpen' command returns more information when working with LIF files.
• (E/P) The STED parameters will be displayed even if no STED license is present.
• (E/P) Replace ":" with "_" in Deconvolution Wizard results to be more compliant with OS file managers.
• (E/P) Add LSM 780 to LSM-specific dialog.
• (All) Skip the STED Z Stabilization (though not the UI option) if the dataset differs too much from vortex STED.
• (All) Minimize the output of the STED Z Stabilization.
• (E/P) Change order of 'Skip' and 'Correct' buttons in Z drift stage (Deconvolution Wizard).
• (C) Add format reports of STED parameters as required by HRM 3.1.
• (E/P) Adapt texts in Chromatic Shift help, dialogs and reports (Batch Processor only).
• (All) 'huOpt getFormatInfo' includes the ND2 file format.
• (E/P) Parameter "Object size" has been renamed as "Penetration Depth" in the SFP renderer.
• (All) New installer for Mac OS X. Installer removes deprecated binaries that depend on the Aqua toolkit.

Bug fixes

• (All) Saving with compression was skipped for the target format ics/ids.
• (E/P) Fix wrong import of the STED 3D parameter when loading old templates in which STED 3D was not present. The default value was wrongly set to 100% whereas it should be 0%.
• (E/P) Report STED parameters in optical tab.
• (All) Fix empty result produced by STED deconvolution + measured PSFs.
• (S) Fix unwanted flip of Y coordinates introduced by Huygens on images provided by MBF Bioscience's clients: 'Stereo Investigator' and 'Neurolucida'.
• (P) Fix icon selector which failed to show when the Huygens preferences contained stitcher entries.
• (E/P/C) Fix bug in the 'huOpt' commands which caused the Batch Processor to stall with STED 3D datasets + Stabilization.
• (E/P) Fix thumbnail menu which (wrongly) grayed out the MIP entry in Freeware mode.
• (All) Fix ZVI reader which failed to read RGB24 and RGB48 types.
• (All) Fix memory leaks in STED deconvolution (sub brick mode).
• (All) Fix in-place and not-in-place stabilization of STED time series (stabilizer tool and "stabilize" command).
• (All) Fix error in STED deconvolution ocurring when NA > R.I. (lens).
• (E/P) Fix Twin Slicer time swing.
• (All) Fix wrong calculation (factor 2) in backprojected pinhole radius (LIF data only).
• (E/P) Fix a reporting problem in the Batch Processor which hid many statements when the license included the STED + Leica features.
• (All) Fix memory leaks in the LIF reader.
• (E/P) Fix 'Done' button which the Deconvolution Wizard missed in a particular (seldom used) route.
• (E/P) Fix bug in MIP renderer happening when the user chose "Customized Render Size" but provided no customized size.
• (E/P) Fix wrong background colors in Parameter Editor (Windows Vista and higher).
• (E/P) Fix brick mode which failed to show the deconvolved results in the corresponding thumbnail (Operations Window and Tcl Shell only).
• (All) Fix 'unspecified' microscope type when opening OME files.
• (E/P) Fix timeout issue in Deconvolution template editor. The value was not properly saved for all channels.
• (E/P) Fix error where Object Analysis results in Colocalization Analyzer could not be saved.