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News

Upcoming SVI-BiteSizeBio webinar: Reliable quantification of fluorescence images

Friday 28 of September, 2018
SVI is happy to announce the webinar "Reliable quantification of fluorescence images: from raw data to reproducible results" (9th of October 2018), organized in collaboration with BiteSizeBio. In this webinar, you will learn how to overcome issues with stage drift and crosstalk between channels, how to obtain reliable images and reproducible quantification results, how to proceed with advanced image analysis after restoring images. During fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blurring and the noise implicit in the image formation are two of the largest sources of experimental trouble. Additional aberrations, such as stage drift, and crosstalk and chromatic aberration between channels, can also affect the imaging. Huygens image deconvolution and restoration is a proven method to revert these issues and recover a more realistic representation of the original object. After restoration of the image, you can proceed with the advanced Huygens analysis options for colocalization, object measurements, and tracking. This webinar will illustrate how you can make optimal use of the complete Huygens workflow to obtain reliable images and reproducible quantification results, focusing on the advanced analysis options offered by the Huygens software.

Subscription is open at this BiteSizeBio page.


Light-sheet Fusion & Deconvolution Webinar, Held September 18th, 2018

Wednesday 19 of September, 2018
Many customers joined this interesting webinar which described the latest developments in Light-sheet/SPIM deconvolution and fusion in Huygens.

They got a general overview plus an update on developments since Spring 2017 when we introduced the first Huygens wizard for Light-sheet images.
The invitation is closed now and customers who subscribed will receive the recording.

Cover and article on Huygens Light Sheet Fusion and Deconvolution Wizard in current Microscopy Today issue

Thursday 13 of September, 2018
Together with several research groups (see co-author list below), SVI has published an article and cover in the September issue of Microscopy Today on "Restoration of Light Sheet Multi-View Data with the Huygens Fusion and Deconvolution Wizard".
The article describes the Huygens Fusion and Deconvolution Wizard, which is a unique integrated solution for deconvolving light sheet microscopy images from various kinds of light sheet systems. Detailed descriptions are given of the fusion of multi-views of the specimen, processing of deskewed of data, and image deconvolution. Examples show significant improvements in image signal and resolution of more than 1.5-2 times.

You can access this Microscopy Today September issue for free: here.
Authors: Peter J. Verveer (1), Vincent T.G. Schoonderwoert (1), Denis Ressnikoff (2), Shane D. Elliott (3), Kiefer D. van Teutem (1), Tobias C. Walther (3) and Hans T.M. van der Voort (1)

1 Scientific Volume Imaging bv, Laapersveld 63, 1213VB, Hilversum, The Netherlands
2 CIQLE, Centre d’imagerie quantitative Lyon-Est, Université Claude Bernard Lyon 1, Lyon, France
3 Department of Genetics and Complex Diseases, Harvard University, Boston, MA

For more information on our Light Sheet Fusion and Deconvolution Wizard you can visit this online wiki page or request a free trial license by downloading Huygens.

Huygens imaging course this September is fully booked - Next course in May 2019

Tuesday 14 of August, 2018
On September 20 and 21, we will have again our Huygens imaging course at our SVI headquarters in Hilversum (The Netherlands).

If you aim for getting the best possible images out of your microscope and in processing them optimally, then this training may be of interest to you.
Topics range from the advised microscope acquisition settings to recognizing imaging artifacts and using Huygens for fixing these. Obviously, we will explain all the Huygens deconvolution, restoration, and analysis options, and have included enough time for hands-on sessions and discussions.

For this course, we allow a maximum of 8 people. This is to ensure that we can give enough attention to all the participants.
More detailed information on this course and quotes from previous participants, can be found here.

If you are interested in joining this course, please send an email to info at svi.nl or call us: 0031 35 642 1626

The SVI team

This course is fully booked, we like to refer you to our May 2019 (9 and 10 to be precise) course for which you can already subscribe.

Important note to Hyvolution 2 users

Monday 06 of August, 2018
In July 2018, reports have reached us that the HyVolution 2 module in the LAS X software may provide partly incorrect multi-channel meta-data to Huygens. This occurs when multi-channel LIF files are read into LASX and subsequently sent to Huygens via Hyvolution2. Please visit our HyVolution page for up-to-date information and suggested alternatives.

What's New Webinar 18.04

Thursday 17 of May, 2018
The Huygens 18.04 What's New Webinar will showcase the new features that are included in the 18.04 Huygens version. In the new version we will introduce a new feature in Huygens: the Batch Express! You simply have to select the desired Watched folder and the images in the folder will be automatically deconvolved by the Batch Express within seconds. The Batch Express supports a wide range of microscope types (widefield, confocal, spinning disk, multiphoton, STED, and a variety of SPIM/Light Sheet systems) and file formats. Compatibility with your image files is not an issue. Additional features and tools in the 18.04 version are:

  • Triplet mode in Twin Slicer (in Basic Mode!)
  • Added “Cold Pixel” mode in Hot Pixel Remover
  • New Lattice Light Sheet Deconvolution
  • Object Stabilizer: added deskew mode for Lattice Light Sheet data, ability to choose a reference frame or slice
  • Added colocalization coefficients in an improved Coloc Analyzer: Li's ICQ, Van Steensel's CCF
  • Native Nikon ND2 and Olympus OIR readers for Windows
  • Multi GPU deconvolution via the Operations Window in Huygens Professional
  • New GPU operations: filters, copy, statistics, histogram operations, slicing, multiMIP, faster deconvolution, visualization, and Deconvolution Express
  • New right-click menu for an easier workflow

You can download Huygens 18.04 from here and you can request a test license after starting Huygens Essential or Professional.

Are you interested in the recording of the webinar? Email sales at svi.nl. For a list of the new features available in 18.04 please consult this webpage.

Release of Huygens Remote Manager 3.5.0

Tuesday 06 of February, 2018
The Huygens Remote Manager is a multi-user web-based interface to the Huygens Core for parallel batch deconvolution. The HRM team and Scientific Volume Imaging are proudly announcing the release of HRM version 3.5.0. New features include:

  • New, modern user interface
  • GMLE deconvolution algorithm
  • Stabilization of deconvolved time series
  • New spherical aberration correction mode
  • New license overview page for admins
  • Performance increase for image selection
  • Bug fixes

Visit the HRM website via this link for more information and enjoy HRM 3.5.0!

HRM