Experimental PSF with the Huygens PSF distiller
By default, Huygens uses a theoretical PSF to deconvolve your image data, so no extra work is needed. When a deconvolution run is started the theoretical PSF will be automatically calculated from the microscopy parameters and used "on the fly".
Huygens also offers the option to load and use an experimental PSF. With the Huygens PSF Distiller Option, you can even make your own experimental PSF from bead images (see Recording Beads). The PSF Distiller is a wizard-based option that assist in properly distilling a PSF from single and multiple channel data. This data can be multiple beads recorded as separate images, multiple beads in the same image, or both.
Chromatic shift, if present, will be reported by the PSF Distiller wizard, and can subsequently be corrected with the Chromatic Aberration Corrector option.
The PSF distiller uses a Non Linear Iterative Method to measure a PSF when your beads are not small enough to provide a direct measurement of the PSF (i.e. they are not Sub Resolution in terms of Band Width).
To learn how it works, refer to Psf From Beads. For a practical tutorial see Using The Psf Distiller.
You can Test The Software to see how it works.