Restoration Parameters

Also called Deconvolution Parameters in the Huygens Software, the Restoration Parameters are those necessary for Doing Deconvolution, together with the Microscopic Parameters that describe the image.

Most general

• Restoration Method
• Point Spread Function image (if not provided, a theoretical PSF will be used instead based on the Microscopic Parameters).

Background

• Background Mode
• Background Per Channel

Iterations control

• Maximum number of iterations
• Signal To Noise Ratio
• Quality Change Threshold
• Iteration Mode
• Bleaching correction
• Brick layout

For scripts

• Time Out

A typical example for a two-channel image could be:

• Restoration Method: Classic Maximum Likelihood Estimation
• Point Spread Function: theoretical
• Signal To Noise Ratio: { 14 7 }
• Background Mode: Manual
• Background Per Channel: { 900 2400 }
• Bleaching Mode: Off
• Brick layout: Auto
• Quality Change Threshold: 0.003
• Max Num Of Iterations: 80
• Iteration Mode: Fast
• Padding Mode: auto
• Time Out: 6000