In the Huygens Software, the data is inspected for Bleaching Effects while doing deconvolution when the user requires so. If a correction is possible it will be applied during the restoration.
Not all images can be automatically corrected for bleaching. Time dependent information must be inside the image for this to happen, and this information is not available in all image types:
- 3D images and time series acquired in widefield microscopes can always be corrected.
- Confocal and Spinning Disk Microscope images can only be corrected if they are part of a Time Series, and when the bleaching over time shows exponential behavior.
The bleaching mode is a restoration parameter that specifies whether this correction is applied (when the image type allows it) or not.
Images affected by large black levels will encounter difficulties when the program estimates the bleaching decay function. To prevent this, the batch processor has an option to correct the black level automatically.
For more details, see correct the bleaching. See also Bleaching vs Sampling.