HUYGENS deconvolution & restoration for Abberior
A direct link from STED imaging to Huygens renowned Deconvolution, Restoration and 3D Analysis!
Both Abberior's STEDYCON and FACILITY line microscopes include a direct connection to Huygens. This Feeder feature allows researchers to get the most out of their STED images, even when SNR levels are below 5, by using Huygens' powerful and renowned MLE deconvolution algorithms. Complete your imaging workflow with modules like the Tile Stitcher, Chromatic Aberration Corrector, and Image (AI) Segmentation and Analysis. All in one stand-alone Huygens Professional package. Since the end of 2024, Aberrior's Matrix Detector images can be further improved with Huygens Matrix STED deconvolution option, withs its unique capacity to increase two-point resolution even further utilizing a unique Super-sampling approach.
Abberior HUYGENS
Feed data directly from Abberior to HUYGENS and deconvolve, restore, and analyze your images with ease.
True MLE deconvolution
Includes a stand-alone HUYGENS Pro package; restoration is powered by the reliable and high-quality Huygens MLE deconvolution algorithms.
Reliable 3D Analysis
Finish your imaging with using Huygens' Colocalization or Object-based Analysis, with AI segmentation tools like Cellpose and SAM2 fully integrated.
Why HUYGENS? Get more from your data!
STED is an intrinsic super-resolution technique (i.e. no post-processing is required to obtain super-resolution) and can easily reach a resolution below 40 nm. However, even with highly sensitive photon-counting detectors, the physics behind STED imaging and the intrinsic photon shot-noise, limit the photon counts per pixel which leads to noisier images compared to the confocal counter-part. This can be partially solved by increasing line-accumulation, with the downside that it slows down image acquisition and causing photo-bleaching issues and cell/sample damage. This is where Huygens will help!
Huygens Professional package, offered with the STEDYCON and FACILITY Line, helps researchers to get more from their confocal and super-resolution STED images, even with low signal and with noisy images. Huygens' CMLE and GMLE deconvolution algorithms perform extremely well on dim and very noisy STED data, facilitating users to image with lower laser power and to use less line-accumulation. In turn this allows users to acquire images much faster, reduce bleaching, and minimize sample damage. Additional imaging issues like channel misalignment, sample drift, tile stitching issues and vignetting, can be corrected with add-on options in Huygens. Together with advanced tools like BioAIsegment (including Cellpose) and SAM2, Huygens offers a full package for optimal segmentation for visualization and analysis.
Improve image acquisition speed
Increase contrast
Remove noise
Reduce background signal
Reduce blur
Increase resolution (lateral and axial!)
Drift correction (stabilization)
Spherical Aberration Correction
Advanced 2D & 3D Visualization & Analysis
Easy tile stitching of overview images
GPU acceleration
Learn more
Connection to HUYGENS Professional a optimized workflow
Huygens now fully supports the OBF data format used for the STEDYCON and FACILITY line. Users can open OBF data directly in Huygens via drag-and-drop or via the regular open command. Huygens automatically reads in all meta-data required for accurate deconvolution and further processing.* The acquisition software of Abberior also introduces a feature that allows users to send any image from their imaging session to Huygens Professional with a single mouse-click. The dataset can be easily deconvolved in Huygens via the fully automated Deconvolution Express, or with the Deconvolution Wizard when the users prefers full manual control on the deconvolution settings. In addition, also the Abberior's MSR files, that is used for their other STED microscopes can be read into Huygens.
* For optimal support of OBF meta-data in Huygens, the data should be acquired with version 4.0 of the STEDYCON software, and Huygens version 19.04 or higher is recommended. Data acquired with version 6.0 of the STEDYCON or with the Abberior Facility Line requires Huygens versions 21.04 or higher. A license flag (I) is required in the Huygens license in order to make use of the Abberior Image Feeder functionality. Contact us if you require a test license.
HUYGENS for Abberior: what's included?
Abberior Instruments offers various Huygens Professional configurations, optimized for their STED microscopes and tailored to fit your various image analysis needs. A powerful, yet very affordable and compact GPU acceleration workstation can be optionally added to your configuration for most efficient image processing. The workstation specs have been selected by SVI and Abberior Instruments specialists to ensure an optimal supported solution.
The HUYGENS for Abberior configurations include a stand-alone Huygens Professional license and includes many useful functionalities such as: Deconvolution Wizard, Deconvolution Express, Workflow Processor and various easy-to-use 2D and 3D visualization tools. The table below provides an overview of the various Huygens packages offered by Abberior Instruments.
It is always possible to extend your Huygens license with additional options. Any Huygens package can be installed on the acquisition system, but it is also possible to install/migrate the Huygens license to a separate workstation or upgrade the license to an floating Huygens Everywhere license that can be used on any computer. If you are interested in any other option not shown in the table below or if you would like to learn more about which Huygens solution suits your research needs best, please contact us.
Download Huygens now!| HUYGENS Essential Stichter Package | HUYGENS Professional Starter Package | HUYGENS Professional Plus Package | HUYGENS Professional Plus Analysis Package | HUYGENS Essential USA package | |
|---|---|---|---|---|---|
| Huygens Essential as stand-alone package. Includes: Deconvolution Wizard, Workflow Processor & many useful visualization tools. |
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| Huygens Professional as stand-alone package. Includes: Deconvolution Wizard, Workflow Processor, Operations Window, Scripting & many useful visualization tools. |
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| Deconvolution Express & Batch Feeder | |||||
| Confocal Optical option | |||||
| STED Optical option | |||||
| Object Stabilizer option | |||||
| Time Option | |||||
| Full File Reader | |||||
| Small GPU option | |||||
| Large GPU option | (Optional) | (Optional) | (Optional) | ||
| Abberior Image Feeder option | |||||
| 1.5 hour Remote training by SVI staff | |||||
| 1 Year Maintenance & Upgrades | |||||
| PSF distiller | |||||
| Crosstalk Corrector | |||||
| Chromatic Aberration Corrector | |||||
| Surface Renderer | |||||
| Movie Maker | |||||
| Colocalization Analyzer (Wizard + Advanced + Compact) |
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| Object Analyzer | |||||
| Tile Stitcher | (Optional) | (Optional) | |||
| Extended Maintenance & Upgrades (3 years total) | (Optional) | (Optional) | (Optional) | (Optional) | (Optional) |
Options marked with a and any other option in Huygens can be optionally added via SVI. Contact SVI for all the possibilities or request a quote.
Get started now!
You are always welcome to try out Huygens deconvolution on your confocal and STED images. When you install the Huygens Suite on your computer, also feel free to try out our other products. You can also request pricing information or contact us for more information about the options best suited for your hardware and image processing needs.
Download Now!STEDYCON + HUYGENS deconvolution Image Gallery