References to Scientific Volume Imaging software

Many scientific papers refer to the use of the SVI's software. Below is just a selection of these papers that were kindly sent to us. More information on our software can be found in these articles. If you have used a SVI product for a publication we would appreciate if the use is describe correctly (see how to cite us). If you have already published an article or book (chapter) with Huygens, we like to congratulate you and ask you to be included in the list below by filling out this form or by sending the reference to support at svi.nl.

What follows are mostly references that Huygens users asked to be included here, but many others are available for example in Google Scholar.

Remote management

A. Ponti, A. Gulati, V. Bäcker and P. Schwarb.
Huygens Remote Manager, a Web Interface for High-Volume Batch Deconvolution.
Imaging & Microscopy (2007) V 9, No. 2, pp 57-58.
unabridged version (distributed with permission)

Theoretical PSFs versus measured PSFs

Landmann, L.
Deconvolution improves colocalization analysis of multiple fluorochromes in 3D confocal data sets more than filtering techniques
Jo. Micr. (2002) V 208, Pt 2, pp 134-147

de Grauw CJ, Vroom JM, van der Voort HTM and Gerritsen HC
Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens
Applied Optics (1999) V 38, No.28, pp

Rothen-Rutishauser BM, Messerli MJ, van der Voort H, Guenthert M and Wunderli-Allenspach, H
Deconvolution Combined with Digital Colocalization Analysis to Study the Spatial Distribution of Tight and Adherens Junction Proteins
Jo. of Computer-Assisted Micr. (1998) V10 No. 3, pp 103-111

Kano H, van der Voort HTM, Schrader M, van Kempen GMP and Hell SW
Avalanche photodiode detection with object scanning and image restoration provides 2-4 fold resolution increase in two-photon fluorescence microscopy
Bioimaging 4 (1996) pp 187-197 get pdf

Huygens Analysis Tools (colocalization, object analysis...)

H. Peuschel, T. Ruckelshausen, C. Cavelius, A. Kraegeloh.
Quantification of internalized silica nanoparticles via STED microscopy.
BioMed Research International, February 2015
Huygens CMLE was used for confocal and STED deconvolution. Huygens Advanced Object Analyzer was applied to segment the cell boundary and to measure the number and geometry of internalized nanoparticles. The authors took the effort to describe the analysis workflow thoroughly.

Dupuis J, Préfontaine A, Villeneuve L, Ruel N, Lefebvre F, Calderone A.
Bone marrow-derived progenitor cells contribute to lung remodelling after myocardial infarction.
Cardiovasc Pathol. 2007 Nov-Dec;16(6):321-8.
get pdf

Ch. Le Clainche, D. Schlaepfer, A. Ferrari1, M. Klingauf, K. Grohmanova1, A. Veligodskiy, D. Didry, D. Le, C. Egile, M.-F. Carlier, R. Kroschewski
IQGAP1 stimulates actin assembly through the N-WASP-ARP2/3 pathway.
J. Biol. Chem, 10.1074
get pdf

Landmann, L.
Deconvolution improves colocalization analysis of multiple fluorochromes in 3D confocal data sets more than filtering techniques
Jo. Micr. (2002) V 208, Pt 2, pp 134-147

Rothen-Rutishauser BM, Messerli MJ, van der Voort H, Guenthert M and Wunderli-Allenspach H
Deconvolution Combined with Digital Colocalization Analysis to Study the Spatial Distribution of Tight and Adherens Junction Proteins
Jo. of Computer-Assisted Micr. (1998) V10 No. 3, pp 103-111

Some references to the Visualization Tools

Maliba R, Brkovic A, Neagoe PE, Villeneuve LR, Sirois MG.
Angiopoietin-mediated endothelial P-selectin translocation: cell signaling
J Leukoc Biol. 2007 Nov 5

A. Shavinskaya, S. Boulant, F. Penin, J. McLauchlan, and R. Bartenschlager
The lipid droplet binding domain of hepatitis C virus core protein is a major determinant for efficient virus assembly
J. Biol. Chem, 10.1074/get pdf

Noemi N. Taniwaki, Fabiana S. Machado, Andre R. Massensini, Renato A. Mortara
Trypanosoma cruzi disrupts myofibrillar organization and intracellular calcium levels in mouse neonatal cardiomyocytes
Cell Tissue Res (2006) artId 152

Bergsma CBJ, Streekstra GJ, Smeulders AWM and Manders EMM
Velocity Estimation of Spots in Three-Dimensional Confocal Image Sequences of Living Cells
Cytometry (2001) 43 pp 261-272

Noordmans HJ, van der Voort HTM and Smeulders AWM
Spectral Volume Rendering
IEEE Transactions On Visualization And Computer Graphics (2002) VOL. 6, NO. 3 pp 196-207

Noordmans HJ, Smeulders AWM and van der Voort HTM
Fast volume render techniques for interactive analysis
The Visual Computer (1997) 13 pp 345-358


Habermann FA (2000)
Untersuchungen zur Anordnung von Makro- und Mikrochromosomen im Zellkern embryonaler Fibroblasten und Neuronen von Gallus domesticus.
Dissertation zum Erwerb des Doktorgrades der Medizin an der Medizinischen Fakultät der Ludwig-Maximilians-Universität zu München.
get pdf

Verveer PJ (1998)
Computational and Optical Methods for Improving Resolution and Signal Quality in Fluorescence Microscopy. Technische Universiteit Delft. abstract and pdf

Papers referring to the use of Huygens


Xiaochao Tan, Priyam Banerjee, Hou-Fu Guo, Stephen Ireland, Daniela Pankova, Young-ho Ahn, Irodotos Michail Nikolaidis, Xin Liu, Yanbin Zhao, Yongming Xue, Alan R. Burns, Jonathon Roybal, Don L. Gibbons, Tomasz Zal, Chad J. Creighton, Daniel Ungar, Yanzhuang Wang, and Jonathan M. Kurie
Epithelial-to-mesenchymal transition drives a pro-metastatic Golgi compaction process through scaffolding protein PAQR11
J Clin Invest. 2017;127(1):117–131. doi:10.1172/JCI88736

Yulong Chen, Houfu Guo, Masahiko Terajima, Priyam Banerjee, Xin Liu, Jiang Yu, Amin A. Momin, Hiroyuki Katayama, Samir M. Hanash, Alan R. Burns, Gregg B. Fields, Mitsuo Yamauchi and Jonathan M. Kurie
Lysyl Hydroxylase 2 Is Secreted by Tumor Cells and Can Modify Collagen in the Extracellular Space
J. Biol. Chem. 2016 291: 25799-25808
The Huygens software was used to deconvolve and visualize the cover of this issue of The Journal of Biological Chemistry.

Zandt B-J, Liu J.H., Veruki M.L., Hartveit E.
AII amacrine cells: quantitative reconstruction and morphometric analysis of electrophysiologically identified cells in live rat retinal slices imaged with multi-photon excitation microscopy.
Brains Structure & Function 1–32 (2016)
This paper describes in much detail the optimimal imaging and deconvolutution settings that were applied.

Kochan J, Wawro M, Kasza A,
IF-combined smRNA FISH reveals interaction of MCPIP1 protein with IER3 mRNA
Biology Open 2016 : doi: 10.1242/bio.018010
Huygens was used for deconvolution and the Huygens Colocalization Analyzer option was applied].

Christian A. Ruge, Adam Bohr, Moritz Beck-Broichsitter, Valérie Nicolas, Nicolas Tsapis, Elias Fattal.
Disintegration of nano-embedded microparticles after deposition on mucus: a mechanistic study, Colloids and Surfaces B: Biointerfaces 139 (2016) 219–227

Barna L, Dudok B, Miczán V, Horváth A, I László Z, and Katona I
Correlated confocal and super-resolution imaging by VividSTORM.
Nature Protocols 11, 163–183 (2016)
Huygens deconvolution was used to create the cover of this issue of Nature Protocols.

Magalie Bénard, Damien Schapman, Alexis Lebon, Baptiste Monterroso, Marine Bellenger, Frank Le Foll, Jennifer Pasquier, Hubert Vaudry, David Vaudry and Ludovic Galas.
Structural and functional analysis of tunneling nanotubes (TnTs) using gCW STED and gconfocal approaches
Biol. Cell (2015) 107, 419–425, DOI: 10.1111/boc.201500004, Short Communication

Martin Weichert, Alexander Lichius, Bert-Ewald Priegnitz, Ulrike Brandt, Johannes Gottschalk, Thorben Nawrath, Ulrike Groenhagen, Nick D. Read, Stefan Schulz, and André Fleißner
Accumulation of specific sterol precursors targets a MAP kinase cascade mediating cell–cell recognition and fusion
PNAS113/42/11877-11882, Published ahead of print, October 5, 2016


Trcek T, Grosch M, York A, Shroff H, Lionnet T, and Lehmann R
Drosophila germ granules are structured and contain homotypic mRNA clusters
Nature Communications 6 (August 2015) doi 10.1038/ncomms8962]
Huygens deconvolution and pixel shift correction was used here.

Kochan J, Wawro M, and Kasza A
Simultaneous detection of mRNA and protein in single cells using immunofluorescence-combined single-molecule RNA FISH
BioTechniques 59:209-221 (October 2015) doi 10.2144/0001143405]

Unnersjö-Jess D., Scott L., Blom H., Brismar H.
Super-resolution stimulated emission depletion imaging of slit diaphragm proteins in optically
cleared kidney tissue.
Kidney International Kidney International, online publication ahead of print, 7 October 2015

Castilho A., Ambrosio A.F., Hartveit E., Veruki M.L.
Disruption of a Neural Microcircuit in the Rod Pathway of the Mammalian Retina by Duabetes Mellitus.
The Journal of Neuroscience 35(13): 5422-5433

Brennan F.H., Gordon R, Lao H.W., Biggins P.J., Taylor S.M., Franklin R.J.M., Woodruff T.M., Ruitenberg, M.J.
The Complement Receptor C5aR Controls Acute Inflammation and Astrogliosis following Spinal Cord Injury.
The Journal of Neuroscience 35(16): 6517-6531
Huygens deconvolution was used to create the cover of this J. Neuroscience issue.

Takuya Azuma and Takayuki Kei
Super-resolution spinning-disk confocal microscopy using optical photon reassignment.
Optics Express. 23(11): 15003.

Besseling, T.H., Jose, J. and Blaaderen, A. V.
Methods to calibrate and scale axial distances in confocal microscopy as a function of refractive index.
Journal of Microscopy, 257: 142–150
This paper describes two methods to calibrate Z sampling of 3D confocal microscopes. See also CalibrateAxialDistances.

Zheng P, Bertolet G, Chen Y, Huang S, Liu D
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer.
J Vis. Exp. issue 96

Piwowarczyk K, Wybieralska E, Baran J, Borowczyk J, Rybak P, Kosińska M, Włodarczyk AJ, Michalik M, Siedlar M, Madeja Z, Dobrucki J, Reiss K, Czyż J
Fenofibrate enhances barrier function of endothelial continuum within the metastatic niche of prostate cancer cells
Expert Opin. Ther. Targets (2015) 19(2):163-176

Ponti A, Sevilla Sanchez D, Belyaev Y
Deconvolution of Very Large Data Sets
Imaging & Microscopy (2015) 12:22-24
This article shows that deconvolution of large datasets like SPIM data, can be easily performed with the Huygens Remote Manager/Core.

Dudok B, Barna L, Ledri M, et al.
Cell-specific STORM super-resolution imaging reveals nanoscale organization of cannabinoid signaling
Nature Neuroscience (2015) 18-1:75-86


Chen J, Zhang Z, Li L, Chen BC, Revyakin A, Hajj B, Legant W, Dahan M, Lionnet T, Betzig E, Tjian R, Liu Z (2014), Single-molecule dynamics of enhanceosome assembly in embryonic stem cells
Cell. 2014 Mar 13;156(6):1274-85. doi: 10.1016/j.cell.2014.01.062.

Brüggen, B., Meyer, A., Boven, F., Weiler, R. and Dedek, K. (2014), Type 2 wide-field amacrine cells in TH::GFP mice show a homogenous synapse distribution and contact small ganglion cells.
European Journal of Neuroscience. doi: 10.1111/ejn.12813

Ting CY, McQueen PG, Pandya N, Lin TY, Yang M, Reddy OV, O'Connor MB, McAuliffe M, Lee CH.
Photoreceptor-derived activin promotes dendritic termination and restricts the receptive fields of first-order interneurons in Drosophila.
Neuron. 2014 Feb 19;81(4):830-846.

Areti Pantazopouloua, Mario Pinara, Xin Xiang, and Miguel A. Peñalva (2014)
Maturation of late Golgi cisternae into RabE(RAB11) exocytic post-Golgi carriers visualized in vivo.
Molecular Biology of the Cell. August 15, 2014 vol. 25 no. 16 2428-2443

Virginie Buggia-Prévot, Celia G Fernandez, Sean Riordan, Kulandaivelu S Vetrivel, Jelita Roseman, Jack Waters, Vytautas P Bindokas, Robert Vassar and Gopal Thinakaran
Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase.
Molecular Neurodegeneration 2014, 9:1 doi:10.1186/1750-1326-9-1

Schiefermeier N, Scheffler JM, de Araujo ME, Stasyk T, Yordanov T, Ebner HL, Offterdinger M, Munck S, Hess MW, Wickström SA, Lange A, Wunderlich W, Fässler R, Teis D, Huber LA
The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.
J Cell Biol. 2014 May 26;205(4):525-40

Thauerer B, Voegele P, Hermann-Kleiter N, Thuille N, de Araujo ME, Offterdinger M, Baier G, Huber LA, Baier-Bitterlich G.
LAMTOR2-mediated modulation of NGF/MAPK activation kinetics during differentiation of PC12 cells.
PLoS One. 2014 Apr 21;9(4):e95863

Wijetunge LS, Angibaud J, Frick A, Kind PC, Nägerl UV
Stimulated Emission Depletion (STED) Microscopy Reveals Nanoscale Defects in the Developmental Trajectory of Dendritic Spine Morphogenesis in a Mouse Model of Fragile X Syndrome.
J Neurosci, 5302-5313 (2014)

Freeman K, Tao W, Sun H, Soonpaa MH, Rubart M
In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging.
J Neuroscience methods 2014, Volume 221: 48–61
Huygens deconvolution and Huygens Colocalization Analyzer was used for this study

Saka SK, Vogts An, Kröhnert K, Hillion F, Rizzoli, SO, Wessels JT (2014)
Correlated optical and isotopic nanoscopy.
Nat Commun. 2014 Apr 10;5:3664

Zhang WI, Röhse H, Rizzoli SO, Opazo, F (2014)
Fluorescent in situ hybridization of synaptic proteins imaged with super-resolution STED microscopy.
published ahead of print April 10, 2014, Microsc. Res. Tech.. doi: 10.1002/jemt.22367

Mace EM, Orange JS (2014)
Lytic immune synapse function requires filamentous actin deconstruction by Coronin 1A. PNAS 2014;
published ahead of print April 23, 2014, doi:10.1073/pnas.1314975111

Mace, EM, Orange, JS
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy.
J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).

Kuijk A, Imhof A, Verkuijlen MHW, Besseling TH, van Eck ERH, van Blaaderen A (2014)
Colloidal Silica Rods: Material Properties and Fluorescent Labeling. Part. Part. Syst. Charact.. doi: 10.1002/ppsc.201300329

Padman BS, Bach M, Ramm G (2014)
An Improved Procedure for Subcellular Spatial Alignment during Live-Cell CLEM.
PLoS ONE 9(4): e95967. 22 April 2014 doi: 10.1371/journal.pone.0095967

Bhuvanendran, S.; Salka, K.; Rainey, K.; Sreetama, S.C.; Williams, E.; Leeker, M.; Prasad, V.; Boyd, J.; Patterson, G.H.; Jaiswal, J.K.; Colberg-Poley, A.M.
Superresolution Imaging of Human Cytomegalovirus vMIA Localization in Sub-Mitochondrial Compartments.
Viruses 2014, 6, 1612-1636.

Poenick S1, Jahnke HG, Eichler M, Frost S, Lilie H, Robitzki AA.
Comparative label-free monitoring of immunotoxin efficacy in 2D and 3D mamma carcinoma in vitro models by impedance spectroscopy.
Biosens Bioelectron. 2014 Mar 15;53:370-6. doi: 10.1016/j.bios.2013.09.048. Epub 2013 Sep 29.


Abrahamsson S, Chen J, Hajj B, Stallinga S, Katsov AY, Wisniewski J, Mizuguchi G, Soule P, Mueller F, Dugast Darzacq C, Darzacq X, Wu C, Bargmann CI, Agard DA, Dahan M, Gustafsson MG.
Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
Nat Methods. 2013 Jan;10(1):60-3. doi: 10.1038/nmeth.2277.

Symes A.J, Eilertsen M, Millar M, Nariculam J, Freeman A, Notara M, Feneley, Patel H.R.H, Masters J.R.W, Ahmed A.
Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue
PLoS One 2013 Dec 27

Westhoff J.H, Giselbrecht S, Schmidts M, Schindler S, Beales P.L, Tönshoff B, Liebel U, Gehrig J.
Development of an Automated Imaging Pipeline for the Analysis of the Zebrafish Larval Kidney
PLoS One 2013 Dec 4;8(12)
This paper nicely shows that Huygens fits perfectly well in high content screening workflows and that Huygens deconvolution benefits image quality and further analysis

Kreutmayer S, Csordas A, Kern J, Maass V, Almanzar G, Offterdinger M, Öllinger R, Maass M, Wick G.
Chlamydia pneumoniae infection acts as an endothelial stressor with the potential to initiate the earliest heat shock protein.
Cell Stress Chaperones. 2013 May;18(3):259-68

Coelho PA, Bury L, Sharif B, Riparbelli MG, Fu J, Callaini G, Gloveremail DM, Zernicka-Goetzemail M.
Spindle Formation in the Mouse Embryo Requires Plk4 in the Absence of Centrioles
Developmental Cell 27:586–597, December 9, 2013
Cover image of the issue was also deconvolved with Huygens Professional

Castañeda V, Cerda M, Santibañez F, Jara J, Pulgar E, Palma K, Lemus CG, Concha M, Hartel S.
Computational Methods for Analysis of Dynamic Events In Cell Migration
Current Molecular Medicine, 2013 - accepted

Wang XD, Su YA, Wagner KV, Avrabos C, Scharf SH, Hartmann J, Wolf M, Liebl C, Kühne C, Wurst W, Holsboer F, Eder M, Deussing JM, Müller MB, Schmidt MV.
Nectin-3 links CRHR1 signaling to stress-induced memory deficits and spine loss.
Nat Neurosci. 2013 Jun;16(6):706-13. Epub 2013 May 5.

Anje Sporbert, Zoltan Cseresnyes, Meike Heidbreder, Petra Domaing, Stefan Hauser, Barbara Kaltschmidt, Christian Kaltschmidt, Mike Heilemann, Darius Widera.
Simple Method for Sub-Diffraction Resolution Imaging of Cellular Structures on Standard Confocal Microscopes by Three-Photon Absorption of Quantum Dots.
PLoS One, 2013 May 21;8(5):e64023

Alireza Ghamari1, Mariëtte P.C. van de Corput, Supat Thongjuea, Wiggert A. van Cappellen, Wilfred van IJcken,Jeffrey van Haren,Eric Soler,Dirk Eick,Boris Lenhard and Frank G. Grosveld.
In vivo live imaging of RNA polymerase II transcription factories in primary cells.
Genes&Development, Accepted March 18, 2013

Lukinavicius G., Umezawa K., Olivier N., Honigmann A., Yang G., Plass T., Mueller V., Reymond L., Corrêa Jr. I.R., Luo Z-G., Schultz G., Lemke E.A., Heppenstall P., Eggeling C., Manley S., Johnsson K.
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.
Nature Chemistry 5, 132–139 (2013)

Lukinavicius G., Lavogina D., Orpinell M., Umezawa K., Reymond L., Garin N., Gönczy P., Johnsson K.
Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex.
Current Biology, 17 January 2013


Trcek T, Chao J.A., Larson D.R.,Park H.Y., Zenklusen D., Shenoy S.M., Singer R.H.
Single-mRNA counting using fluorescent in situ hybridization in budding yeast.
Nature Protocols 7, 408–419 (2012)

Malide D, Metais J-Y, Dunbar C.E.
Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by five fluorescent proteins using confocal and multiphoton microscopy.
Blood Sept. 2012, 440636

Micutkova L, Hermann M, Offterdinger M, Hess MW, Matscheski A, Pircher H, Mück C, Ebner HL, Laich A, Ferrando-May E, Zwerschke W, Huber LA, Jansen-Dürr P.
Analysis of the cellular uptake and nuclear delivery of insulin-like growth factor binding protein-3 in human osteosarcoma cells.
Int J Cancer. 2012 Apr 1;130(7):1544-57

Lalle M, Camerini S, Cecchetti S, Sayadi A, Crescenzi M, Pozio E.
Interaction network of the 14-3-3 protein in the ancient protozoan parasite Giardia duodenalis.
J Proteome Res. 2012 May 4;11(5):2666-2683

Mariëtte P. C. van de Corput, Ernie de Boer, Tobias A. Knoch, Wiggert A. van Cappellen, Adrian Quintanilla,Leanna Ferrand, Frank G. Grosveld.
Super-resolution imaging reveals 3D folding dynamics of the β-globin locus upon gene activation
Journal of Cell Science 108522; Advance Online Publication July 5, 2012

F Spira, NS Mueller, G Beck, P von Olshausen, J Beig, R Wedlich-Söldner.
Patchwork organization of the yeast plasma membrane into numerous coexisting domains.
Nature Cell Biology April 29, 2012 vol. 14(6):640-648

Farideh Beigi et al.
Dynamic denitrosylation via S-nitrosoglutathione reductase regulates cardiovascular function.
PNAS March 13, 2012 vol. 109 no. 11 4314-4319

W. Tao, J. Shi, G.W. Dorn, L. Wei. M Rubart
Spatial variability in T-tubule and electrical remodeling of left ventricular epicardium in mouse hearts with transgenic Gαq overexpression-induced pathological hypertrophy.
J Mol Cell Cardiol. Jun 21, 2012 Epub ahead of print

T. Moutinho-Santos, C. Conde, C.E. Sunkel
POLO ensures chromosome bi-orientation by preventing and correcting erroneous chromosome-spindle attachments.
J. Cell Sci. 2012 Feb 1;125(Pt 3):576-583

É.R. Ferreira, A. Bonfim-Melo, R.A. Mortara, D. Bahia
Trypanosoma cruzi extracellular amastigotes and host cell signaling: more pieces to the puzzle.
Front. Immun., 30 Nov 2012, 3:1-6

J-P. Vilardaga, T.J. Gardella, V.L. Wehbi, T.N. Feinstein
Non-canonical signaling of the PTH receptor.
Trends in Pharmacological Sciences, 18 June 2012
Trends in Pharmacological Sciences August issue. Online 18 June 2012
The cover of this issue shows a Huygens deconvolved and alpha blended three-color live endosome.


N Hégarat, E Smith, G Nayak, S Takeda, PA Eyers, H Hochegger.
Aurora A and Aurora B jointly coordinate chromosome segregation and anaphase microtubule dynamics. Includes cover image that was deconvolved with Huygens.
J Cell Biol. 2011 Dec 26;195(7):1103-13

Lalle M, Rosati MA, Bien J, Hehl AB, Pozio E, Tosini F.
Expression of Cryptosporidium parvum Cpa135/CpCCP1 chimeras in Giardia duodenalis: Organization of the protein domains affects the protein secretion pathway.
Exp Parasitol. 2011 Mar;127(3):680-686

Lalle M, Camerini S, Cecchetti S, Blasetti Fantauzzi C, Crescenzi M, Pozio E.
Giardia duodenalis 14-3-3 protein is polyglycylated by a tubulin tyrosin ligase-like member and deglycylated by two metallocarboxypeptidases.
J Biol Chem. 2011 Feb 11;286(6):4471-4484

Lalle M, Currà C, Ciccarone F, Pace T, Cecchetti S, Fantozzi L, Ay B, Breton CB, Ponzi M.
Dematin, a component of the erythrocyte membrane skeleton, is internalized by the malaria parasite and associates with Plasmodium 14-3-3.
J Biol Chem. 2011 Jan 14;286(2):1227-1236

OA Gorleku, AM Barns, GR Prescott, J Greaves, LH Chamberlain.
Endoplasmic reticulum localization of DHHC palmitoyltransferases mediated by lysine-based sorting signals.
J Biol Chem. 2011 Nov 11;286(45):39573-84

J Greaves, JA Carmichael, LH Chamberlain.
The palmitoyl transferase DHHC2 targets a dynamic membrane cycling pathway: regulation by a C-terminal domain.
Mol Biol Cell. 2011 Jun 1;22(11):1887-95

R Peravali, J Gehrig, S Giselbrecht, D Lütjohann, Y Hadzhiev, F Müller, and U Liebel.
Automated feature detection and imaging for high-resolution screening of zebrafish embryos.
BioTechniques, Vol. 50, No. 5, May 2011, pp. 319–324

S Roizard, C Danelon, G Hassaïne, J Piguet, K Schulze, R Hovius, R Tampé, and H Vogel.
Activation of G-Protein-Coupled Receptors in Cell-Derived Plasma Membranes Supported on Porous Beads.
J Am Chem Soc. 2011 Oct 26;133(42):16868-74

E Smith, N Hégarat, C Vesely, I Roseboom, C Larch, H Streicher, K Straatman, H Flynn, M Skehel, T Hirota, R Kuriyama and H Hochegger.
Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1.
The EMBO Journal (2011) 30, 2233 - 2245, Published online 26 April, 2011
This paper nicely describes the use of Huygens Deconvolution for images acquired with a DeltaVision microscope

L Mensa, G Crespo, MJ Gastinger, J Kabat, S Pérez-del-Pulgar, R Miquel, SU Emerson, RH Purcell, X Forns.
Hepatitis C virus receptors claudin-1 and occludin after liver transplantation and influence on early viral kinetics.
Hepatology (2011) May;53(5):1436-1445

T Feinstein,V Wehbi, J Ardura, D Wheeler, S Ferrandon, T Gardella and J-P Vilardaga.
Retromer terminates the generation of cAMP by internalized PTH receptors.
Nature Chemical Biology 2011 March 27; (7):278-284

R Alonso, C Mazzeo, M C Rodriguez, M Marsh, A Fraile-Ramos, V Calvo, A Avila-Flores, I Merida and M Izquierdo.
Diacylglycerol kinase α regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes.
Cell Death and Differentiation 2011 Jan 21, advance online publication

Jeffrey Kamykowski, Peter Carlton, Siddharth Sehgal and Brian Storrie.
Quantitative immunofluorescence mapping reveals little functional
coclustering of proteins within platelet α-granules.
Blood 2011 118: 1370-1373.Prepublished online May 26, 2011

Lei Lu NTU Singapore, Mark S. Ladinski and Tomas Kirchhausen.
Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear
pore assembly.
JCB published August 8, 2011

Vladimir P. Baklaushev, Gaukhar M. Yusubalieva, Eugene B. Tsitrin, Olga I. Gurina, Nadezhda Ph. Grinenko2, Ilya V. Victorov2, and Vladimir P. Chekhonin.
Visualization of Connexin 43-positive cells of glioma and the periglioma zone by means of intravenously injected monoclonal antibodies.
Drug Delivery, 2011, 1–7, Early Online

Schlapp G, Scavone P, Zunino P and S Härtel.
Development of 3D Architecture of Uropathogenic Proteus mirabilis Batch Culture Biofilms - A Quantitative Confocal Microscopy Approach.
J Microbiol Methods, (2011, 87:2, 234-240 (Abstract


Gonzalez D.R., Treuer A.V., Castellanos J., Dulce R.A., and Hare J.M.
Impaired S-Nitrosylation of the Ryanodine Receptor Caused by Xanthine Oxidase Activity Contributes to Calcium Leak in Heart Failure
Journal of Biological Chemistry September 10, 2010, vol 285, 28938-28945

Lalle M, Bavassano C, Fratini F, Cecchetti S, Boisguerin P, Crescenzi M, Pozio E.
Involvement of 14-3-3 protein post-translational modifications in Giardia duodenalis encystations.
Int J Parasitol. (2010), 40, 201-213

Lienkamp S, Ganner A, Boehlke C, Schmidt T, Arnold SJ, Schäfer T, Romaker D, Schuler J, Hoff S, Powelske C, Eifler A, Krönig C, Bullerkotte A, Nitschke R, Kuehn EW, Kim E, Burkhardt H, Brox T, Ronneberger O, Gloy J, Walz G.
Inversin relays Frizzled-8 signals to promote proximal pronephros development.
Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20388-93

Greaves J, Gorleku OA, Salaun C, Chamberlain LH.
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Jane E Leadsham and Campbell W Gourlay
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Q le Duc Q, Q Shi, I Blonk, A Sonnenberg, N Wang, D Leckband, J de Rooij.
Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner.
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Marvin Bentley, Deborah C. Nycz, Ashwini Joglekar, Ismene Fertschai, Roland Malli, Wolfgang F. Graier, and Jesse C. Hay
Vesicular Calcium Regulates Coat Retention, Fusogenicity, and Size of Pre-Golgi Intermediates
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Armin H. Seidl, Edwin W Rubel, and David M. Harris. Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology–Head and Neck Surgery, University of Washington, Seattle, Washington.
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Ramirez O, García A, Rojas R, Couve A & S Härtel
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Nina Jährling, Klaus Becker and hans-Ulrich Dodt. Published December 2009, 3D-reconstruction of blood vessels by
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Oscillatory recruitment of signaling proteins to cell tips promotes coordinated behavior during cell fusion.

Gernot Stocker, Maria Fischer, Dietmar Rieder, Gabriela Bindea, Simon Kainz, Michael Oberstolz, James G McNally and Zlatko Trajanoski
iLAP: a workflow-driven software for experimental protocol development, data acquisition and analysis
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Christoph Hudemann, Maria Elisabet Lönn, José Rodrigo Godoy, Farnaz Zahedi Avval, Francisco Capani, Arne Holmgren, Christopher Horst Lillig
Identification, Expression Pattern, and Characterization of Mouse Glutaredoxin 2 Isoforms
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Jose A. Guerrero, Mark Kyei, Susan Russell, Junling Liu, T. Kent Gartner, Brian Storrie and Jerry Ware
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Patricia A. Soucy, Lewis H. Romer
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M.Oberlaender, P.J. Broser, B. Sakmann & S.Hippler
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Lei Lu NTU Singapore, Mark S. Ladinsky and Tom Kirchhausen.
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Fidorra M, Garcia A, Ipsen J, Härtel S & L Bagatolli.
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Eiko K. de Jong, Jonathan Vinet, Vesna S. Stanulovic, Michel Meijer, Evelyn Wesseling, Klaas Sjollema, Hendrikus W. G. M. Boddekeand Knut Biber
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Nagotu S, Krikken AM, Otzen M, Kiel JA, Veenhuis M, van der Klei IJ
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Sabrina Sonda,* Sasa Stefanic, and Adrian B. Hehl
A Sphingolipid Inhibitor Induces a Cytokinesis Arrest and Blocks Stage Differentiation in Giardia lamblia{triangledown}
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Sylvain Hugel, Mathias Abegg, Vincenzo de Paola, Pico Caroni, Beat H. Gähwiler and R. Anne McKinney
Dendritic Spine Morphology Determines Membrane-Associated Protein Exchange between Dendritic Shafts and Spine Heads
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S. Kumar, G. Ho, K. M. Woo, and L. Zhuo
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Alberto Sanchez-Diaz, Vanessa Marchesi, Stephen Murray, Richard Jones, Gislene Pereira1, Ricky Edmondson, Terry Allen & Karim Labib.
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Mice Lacking Histone Deacetylase 6 Have Hyperacetylated Tubulin but Are Viable and Develop Normally
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D. Wüstner
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Gendron ME, Thorin-Trescases N, Villeneuve L, Thorin E.
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Tony Shmygol, K. Noble and Susan Wray.
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Sandra Goetze, Julio Mateos-Langerak, Hinco J. Gierman, Wim de Leeuw, Osdilly Giromus, Mireille H. G. Indemans, Jan Koster, Vladan Ondrej, Rogier Versteeg, and Roel van Driel
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Siddhart Sehgal, Brian Storrie
Evidence that differential packaging of the major platelet granule proteins von Willebrand factor and fibrinogeen can support their differential release. Journal of Thrombosis and Haemostasis July 2007.

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G.c. White II and R. Rompietti
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Hans-Ulrich Dodt, Ulrich Leischner, Anja Schierloh, Nina Jaerling, Christoph Peter Mauch, Katrin Deininger, Jan Michael Deussing, Matthias Eder, Walter Zieglgaensberger, Klaus Becker.
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Mariette P.C.van de Corput, Tobias A.Knoch, Gert W.van Cappellen, Ernie de Boer, Frank G. Grosveld. (Erasmus MC Cell Biology & Genetics)
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Staci A. Sorensen and Edwin W. Rubel
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Miguel Vicente, Ana Isabel Rico, Rocío Martínez-Arteaga, and Jesús Mingorance
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Seiichiro Nagai, Tadashi Mabuchi, Shuri Hirata, Tomoko Shoda,Tsuyoshi Kasai, Sadaki Yokota, Hiroshi Shitara, Hiromichi Yonekawa, Kazuhiko Hoshi.
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Ann Icking, Monia Amaddii, Mika Ruonala, Stefan Höning, Ritva Tikkanen
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Ilaria Dal Pra, Anna Chiarini, Edward F. Nemeth, Ubaldo Armato.
Roles of Ca(2+)and the Ca(2+)- Sensing Receptor (CARS) in the Expression of Inducible NOS ( Nitric Oxide Synthase)-2 and Its BH4 (Tetrahydrobiopterin)-Dependent Activation in Cytokine-Stimulated Adult Human Astrocytes.
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Anna Chianini, Ilaria Dalpra, Lia menapace, Raffaella Pacchiana, James F. Whitfield and Ubaldo Armato.
Solube amyloid beta-peptide and myelin basic protein strongly stimulate, alone and in synergism with combined pro-inflammatory cytokines, the expression of functional nitric oxide synthase-2 in normal adult human astrocytes.
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Boivin B, Villeneuve LR, Farhat N, Chevalier D, Allen BG
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Sung Wu Rhee, Tregei Starr, Kimberly Forsten-Williams and Brian Storrie
The Steady-State Distribution of Glycosyltransferases Between the Golgi Apparatus and the Endoplasmic Reticulum is Approximately 90:10
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A. A. Aravin, M. S. Klenov, V. V. Vagin, F. Bantignies, G. Cavalli, and V. A. Gvozdev
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R. R. Duncan, A. Bergmann, M. A. Cousin, D. K. Apps & M. J. Shipston
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Yaron Shav-Tal ( [http://singerlab.org/people/ys.htm] ), Xavier Darzacq, Shailesh M Shenoy, Dahlene Fusco, Susan M Janicki, David L Spector, Robert H Singer
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Aten JA, Stap J, Krawczyk PM, van Oven CH, Hoebe RA, Essers J and Kanaar R
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Baunaure F, Eldin P, Cathiard A-M and Vial H
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Callahan R, Labunskiy DA, Logvinova A, Abdallah M, Liu C, Cotten JF and Yosta CS
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Das V, Nal B, Dujeancourt A, Thoulouze MI, Galli T, Roux P, Dautry-Varsat A and Alcover A
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Ehrlich JR, Pourrier M, Weerapura M, Ethier N, Marmabachi M, Hébert TE and Nattel S
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Engstler M, Thilo L, Weise F, Grünfelder CG, Schwarz H, Boshart M and Overath P
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Fischer H, Schwarzer C and Illek B
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Frieden M, James D, Castelbou C, Danckaert A, Martinou JC and Demaurex N
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Grailhe R, de Carvalho LP, Paas Y, Le Poupon C, Soudant M, Bregestovski P, Changeux JP and Corringer, PJ.
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Martinez-Campos M, Basto R, Baker J, Kernan M and Raff JW
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Planat-Bénard V, Menard C, André M, Puceat M, Perez A, Garcia-Verdugo J-M, Pénicaud L and Casteilla, L.
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Richards DA, de Paola V, Caroni P, Gähwiler BH and McKinney RA
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Roux P, Münter S, Frischknecht F, Herbomel P and Shorte SL
Focusing light on infection in four dimensions
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Sedarat F, Lin E, Moore E and Tibbits G
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Schutte B, Henfling M, Kölgen W, Bouman M, Meex S, Leers MPG, Nap M, Björklund V, Björklund P, Björklund B, Lane EB, Omary MB, Jörnvall H and Ramaekers FCS
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Shin JH, Gardel ML, Mahadevan L, Matsudaira P and Weitz DA
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Smets I, Caplanusi A, Despa S, Molnar Z, vandeVen M, Ameloot M and Steels P
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Tschachler E, Reinisch CM, Mayer C, Paiha K, Lassmann H and Weninger W
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Wouterlood FG, van Haeften T, Eijkhoudt M, Baks-te-Bulte L, Goede PH and Witter MP
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Zaccheo O, Dinsdale D, Meacock PA and Glynn P
Neuropathy Target Esterase and Its Yeast Homologue Degrade Phosphatidylcholine to Glycerophosphocholine in Living Cells
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Ausma J, van der Velden HMW, Lenders M-H, van Ankeren EP, Jongsma HJ, Ramaekers FCS, Borgers M and Allessie MA
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Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck
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Balthasar N, Mery P-F, Magoulas CB, Mathers KE, Martin A, Mollard P and Robinson ICAF
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Bantignies F, Grimaud C, Lavrov S, Gabut M, Cavalli G.
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Boivin B, Chevalier D, Villeneuve LR, Rousseau E and Allen BG
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Bürgi S, Baltensperger K and Honegger UE
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Daly CJ and McGrath JC
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Evans JG, Correia I, Krasavina O, Watson N and Matsudaira P
Macrophage podosomes assemble at the leading lamella by growth and fragmentation
The Journal of Cell Biology (2003) Volume 161, Number 4, pp 697-705

Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard J-M, Singer RH and Bertrand E
Single mRNA Molecules Demonstrate Probabilistic Movement in Living Mammalian Cells
Current Biology (2003) Vol 13, pp 161-167

Galvan V, Logvinova A, Sperandio S, Ichijo H and Bredesen DE
Type 1 Insulin-like Growth Factor Receptor (IGF-IR) Signaling Inhibits Apoptosis Signal-regulating Kinase 1 (ASK1)
J.Biol.Chem. (2003) Vol. 278, No. 15, pp 13325-13332

Gerzanich V, Ivanov A, Ivanova S, Yang JB, Zhou H, Dong Y and Simard JM
Alternative Splicing of cGMP-Dependent Protein Kinase I in Angiotensin-Hypertension Novel Mechanism for Nitrate Tolerance in Vascular Smooth Muscle
Circ Res. (2003) 93, pp 805-812

Gerzanich V, Ivanova S, van der Heiden M, Zhou H and Simard JM
Trans-Cellular Proliferating Cell Nuclear Antigen Gene Activation in Cerebral Vascular Smooth Muscle by Endothelial Oxidative Injury In Vivo
Arterioscler Thromb Vasc Biol. (2003) 23, pp

Gimona M, Kaverina I, Resch GP, Vignal E and Burgstaller G
Calponin Repeats Regulate Actin Filament Stability and Formation of Podosomes in Smooth Muscle Cells
Molecular Biology of the Cell (2003) Vol 14, pp

Goshima G and Vale RD
The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line
The Journal of Cell Biology (2003) Volume 162, Number 6 pp

Grünfelder CG, Engstler M, Weise F, Schwarz H, Stierhof Y-D, Morgan GW, Field MC and Overath P
Endocytosis of a Glycosylphosphatidylinositol-anchored Protein via Clathrin-coated Vesicles, Sorting by Default in Endosomes, and Exocytosis via RAB11-positive Carriers
Molecular Biology of the Cell (2003) Vol. 14, pp 2029-2040

Hauf S, Cole RW, LaTerra S, Zimmer C, Schnapp G, Walter R, Heckel A, van Meel J, Rieder CL, and Peters J-M
The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore microtubule attachment and in maintaining the spindle assembly checkpoint
The Journal of Cell Biology (2003), Vol 161( 2), pp 281-294

Heilbronn R, Engstler M, Weger S, Krahn A, Schetter C and Boshart M
ssDNA-dependent colocalization of adeno-associated virus Rep and herpes simplex virus ICP8 in nuclear replication domains
Nucleic Acids Research (2003); Vol. 31, No. 21, pp

Homma N, Takei Y, Tanaka Y, Nakata T, Terada S, Kikkawa M, Noda Y and Hirokawa N
Kinesin Superfamily Protein 2A (KIF2A) Functions in Suppression of Collateral Branch Extension
Cell (2003) Vol 114, Issue 2, pp 229-239

Houzet L, Battini JL, Bernard E, Thibert V, Mougel M.
A new retroelement constituted by a natural alternatively spliced RNA of murine replication-competent retroviruses
EMBO J. (2003) 15;22(18) pp

Jaz´win´ska A, Ribeiro C and Affolter M
Epithelial tube morphogenesis during Drosophila tracheal development requires Piopio, a luminal ZP protein
Nature Cell Biology (2003) 5(10) pp 895-901

Klauke N, Smith GL and Cooper J
Stimulation of Single Isolated Adult Ventricular Myocytes within a Low Volume Using a Planar Microelectrode Array
Biophysical Journal (2003) Vol.85 pp

Lakemond CMM, de Jongh HHJ, Paques M, van Vliet T, Gruppen H, Alphons and Voragen GJ
Gelation of soy glycinin; influence of pH and ionic strength on network structure in relation to protein conformation
Food Hydrocolloids (2003) Vol 17(3) pp 365-377

Lalevee N, Resin V, Arnaudeau S, Demaurex N, Rossier MF
Intracellular Transport of Calcium from Plasma Membrane to Mitochondria in Adrenal H295R Cells: Implication for Steroidogenesis
Endocrinology (2003) ;144(10), pp

Lorger M, Engstler M, Homann M and Göringer HU
Targeting the Variable Surface of African Trypanosomes with Variant Surface Glycoprotein-Specific, Serum-Stable RNA Aptamers
Eukarotic Cell (2003) pp 84-94

Martin-Verdeaux S, Pombo I, Iannascoli B, Roa M, Varin-Blank N, Rivera J and Blank U
Evidence of a role for Munc18-2 and microtubules inmast cell granule exocytosis
Journal of Cell Science (2003) 116, pp 325-334

Malli R, Frieden F, Osibow K, Zoratti C, Mayer M, Demaurex N and Graier WF
Sustained Ca2+ Transfer across Mitochondria Is Essential for Mitochondrial Ca2+ Buffering, Store-operated Ca2+ Entry, and Ca2+ Store Refilling
J. Biol. Chem. (2003) 278: pp

Metzen E, Berchner-Pfannschmidt U, Stengel P, Marxsen JH, Stolze I, Klinger M, Huang WQ, Wotzlaw C, Hellwig-Bürgel T, Jelkmann W, Acker H and Fandrey J
Intracellular localisation of human HIF-1a hydroxylases: implications for oxygen sensing
Journal of Cell Science (2003) 116, pp

Mußmann R, Janssen H, Calafat J, Engstler M, Ansorge I, Clayton C and Borst P
The expression level determines the surface distribution of the transferrin receptor in Trypanosoma brucei
Molecular Microbiology (2003) 47(1) pp 23-35

Nair P, Schaub BE and Rohrer J
Characterization of the Endosomal Sorting Signal of the Cation-Dependent Mannose 6-Phosphate Receptor
J.Biol.Chem. (2003) Vol 278, No. 27 pp

Oleynikov Y and Singer RH
Real-Time Visualization of ZBP1 Association with beta-actin mRNA during Transcription and Localization
Current Biology (2003) Vol. 13 pp 199-207

Perez-Terzic C, Behfar A, Mery A, van Deursen JMA, Terzic A, Pucéat M
Structural Adaptation of the Nuclear Pore Complex in Stem Cell-Derived Cardiomyocytes
Circ Res (2003) 92 pp 444-452

Planat-Bénard V, Menard C, André M, Pucéat M, Perez A, Garcia-Verdugo J-M, Pénicaud L and Casteilla L
Spontaneous Cardiomyocyte Differentiation From Adipose Tissue Stroma Cells
Circ. Res. (2003); 10.1161/01.RES..43271.47

Pucéat M, Travo P, Quinn MT and Fort P
A Dual Role of the GTPase Rac in Cardiac Differentiation of Stem Cells
Molecular Biology of the Cell (2003) Vol. 14, pp

Regnard C, Fesquet D, Janke C, Boucher D, Desbruyeres E, Koulakoff A, Insina C, Travo P, Edde B
Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase
J Cell Sci. (2003);116(Pt 20), pp

Rogers SL, Wiedemann U, Stuurman N and Vale RD
Molecular requirements for actin-based lamella formation in Drosophila S2 cells
The Journal of Cell Biology (2003) Volume 162, Number 6 pp

Stepanova T, Slemmer J, Hoogenraad CC, Lansbergen G, Dortland B, De Zeeuw CI, Grosveld F, van Cappellen G, Akhmanova A and Galjart N
Visualization of Microtubule Growth in Cultured Neurons via the Use of EB3-GFP (End-Binding Protein 3-Green Fluorescent Protein)
The Journal of Neuroscience (2003) 23(7) pp

Thorn H, Stenkula KG, Karlsson M, Örtegren U, Nystrom FH, Gustavsson J and Strålfors P
Cell surface orifices of caveolae and localization of caveolin to the necks of caveolae in adipocytes
Mol. Biol. Cell (2003) 14 pp

Verschure PJ, van der Kraan I, Manders EMM, Hoogstraten D, Houtsmuller AB and van Driel R
Condensed chromatin domains in the mammalian nucleus are accessible to large macromolecules
EMBO reports (2003) 4, pp 861-866

Voet T, Liebe B, Labaere C, Marynen P and Scherthan H
Telomere-independent homologue pairing and checkpoint escape of accessory ring chromosomes in male mouse meiosis
The Journal of Cell Biology (2003) Vol. 162, Number 5, pp 795-807

Wouterlood FG , Böckers T and Witter MP
Synaptic contacts between identified neurons visualized in the confocal laserscanning microscope. Neuroanatomical tracing combined with immunofluorescence detection of postsynaptic density proteins and target neuron-markers
J. Neuroscience Methods (2003) 128 pp 129-142


Arnaudeau S, Frieden M, Nakamura K, Castelbou C, Michalak M and Demaurex N
Calreticulin differentially modulates calcium uptake and release in the endoplasmic reticulum and mitochondria
J. Biol.Chem. (2002) 277 pp

Behfar A, Zingman LV, Hodgson DM, Rauzier J-M, Kane GC, Terzic A and Pucéat M
Stem cell differentiation requires a paracrine pathway in the heart
The FASEB Journal (2002) 16 pp

Brockhaus J and Deitmer JW
Long-lasting modulation of synaptic input to Purkinje neurons by Bergmann glia stimulation in rat brain slices
Journal of Physiology (2002) 545.2 pp 581-593

Charrasse S, Meriane M, Comunale F, Blangy A and Gauthier-Rouvière C
N-cadherin-dependent cell-cell contact regulates Rho GTPases and ß-catenin localization in mouse C2C12 myoblasts
The Journal of Cell Biology (2002) Volume 158, Number 5, pp 953-965

Claycomb JM, MacAlpine DM, Evans JG, Bell SP and Orr-Weaver TL
Visualization of replication initiation and elongation in Drosophila
The Journal of Cell Biology (2002), Volume 159, Number 2, pp 225-236

Conte N, Charafe-Jauffret E, Delaval B, Adélaïde J, Ginestier C, Geneix J, Isnardon D, Jacquemier J and Birnbaum D
Carcinogenesis and translational controls: TACC1 is down-regulated in human cancers and associates with mRNA regulators
Oncogene (2002) 21, pp 5619 - 5630

De Renzis S, Sönnichsen B and Zerial M
Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes
Nature Cell Biology (2002) 4, pp 124-133

Dzwinel W, Yuen DA and Boryczko K
Mesoscopic dynamics of colloids simulated with dissipative particle dynamics and fluid particle model
J Mol Model (2002) 8, pp 33-43

Edinger RS, Watkins SC, Pearce D and Johnson JP
Effect of immunosuppressive agents on glucocorticoid receptor function in A6 cells
Am J Physiol Renal Physiol (2002) 283, pp F254-F261

Engel U, Oezbek S, Engel R, Petri B, Lottspeich F and Holstein TW
Nowa, a novel protein with minicollagen Cys-rich domains, is involved in nematocyst formation in Hydra
Journal of Cell Science (2002) 115, pp

Estrach S, Schmidt S, Diriong S, Penna A, Blangy A, Fort P and Debant A
The Human Rho-GEF Trio and Its Target GTPase RhoG Are Involved in the NGF Pathway, Leading to Neurite Outgrowth
Current Biology (2002), Vol. 12, pp 307-312 get pdf

Furstoss O, Dorey K, Simon V, Barila D, Superti-Furga G and Roche S
c-Abl is an effector of Src for growth factor-induced c-myc expression and DNA synthesis
The EMBO Journal (2002) Vol 21 No 4 pp 514-524

Furuyashiki T, Arakawa Y, Takemoto-Kimura S, Bito H and Narumiya S
Multiple spatiotemporal modes of actin reorganization by NMDA receptors and voltage-gated Ca21 channels
PNAS (2002) vol.99 no. 22 pp

Gräf, R.
DdNek2, the first non-vertebrate homologue of human Nek2, is involved in the formation of microtubuleorganizing centers
Journal of Cell Science (2002) 115, pp
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Grünfelder CG, Engstler M, Weise F, Schwarz H, Stierhof Y-D, Boshart M and Overath P
Accumulation of a GPI-Anchored Protein at the Cell Surface Requires Sorting at Multiple Intracellular Levels
Traffic (2002), 3, pp 547-559

Haj FG, Verveer PJ, Squire A, Neel BG and Bastiaens PIH
Imaging Sites of Receptor Dephosphorylation by PTP1B on the Surface of the Endoplasmic Reticulum
Science (2002) pp

Hannus M, Feiguin F, Heisenberg C-F and Eaton S
Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A
Development (2002) 129, pp

Kern G, Bösch ST, Unterhuber E and Pelster B
Mechanisms of acid secretion in pseudobranch cells of rainbow trout Oncorhynchus
The Journal of Experimental Biology (2002) 205 pp 2943-2954

Koberna K, Malínský J, Pliss A, Masata M, Vecerová J, Fialová M, Bednár J and Raska I
Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of "Christmas trees" in situ
The Journal of Cell Biology (2002) Volume 157, Number 5, pp 743-748 get pdf

de Krom M,van de Corput M, von Lindern M, Grosveld F and Strouboulis J
Stochastic Patterns in Globin Gene Expression Are Established prior to Transcriptional Activation and Are Clonally Inherited
Molecular Cell (2002) Vol. 9, pp
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Landmann, L.
Deconvolution improves colocalization analysis of multiple fluorochromes in 3D confocal data sets more than filtering techniques
Jo. Micr. (2002) V 208, Pt 2, pp 134-147

Li J, Pucéat M, Perez-Terzic C, Mery A, Nakamura K, Michalak M, Krause K-H and Jaconi ME
Calreticulin reveals a critical Ca2+ checkpoint in cardiac myofibrillogenesis
The Journal of Cell Biology (2002) Volume 158, Number 1, pp 103-113 get pdf

Malínský J, Koberna K, Bednár J, Stulík J and Raska I
Searching for active ribosomal genes in situ: light microscopy in light of the electron beam
Journal of Structural Biology (2002) 140 pp 227-231

Mary S, Charrasse S, Meriane M, Comunale F, Travo P, Blangy A and Gauthier-Rouvière C
Biogenesis of N-Cadherin-dependent Cell-Cell Contacts in Living Fibroblasts Is a Microtubuledependent Kinesin-driven Mechanism
Molecular Biology of the Cell Vol. (2002) 13, pp 285-301 get pdf

Polesello C, Delon I, Valenti P, Ferrer P and Payre F
Dmoesin controls actin-based cell shape and polarity during Drosophila melanogaster oogenesis
Nature Cell Biology (2002) 4, pp 782-789

Rehberg, M. and Gräf, R.
Dictyostelium EB1 Is a Genuine Centrosomal Component Required for Proper Spindle Formation
Molecular Biology of the Cell (2002) Vol. 13, pp
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Rothen-Rutishauser B, Riesen FK, Braun A, Gunthert M, Wunderli-Allenspach H.
Dynamics of tight and adherens junctions under EGTA treatment.
J Membr Biol. (2002) Jul 15;188(2):151-62

Stahl A, Evans JG, Pattel S, Hirsch D and Lodish HF
Insulin Causes Fatty Acid Transport Protein Translocation and Enhanced Fatty Acid Uptake in Adipocytes
Developmental Cell (2002) 2, pp 477-488

Verschure PJ, van der Kraan I, Enserink JM, Moné MJ, Manders EMM and van Driel R
Large-scale Chromatin Organization and the Localization of Proteins Involved in Gene Expression in Human Cells
J.Histochem.Cytochem. (2002) 50, pp

Verveer, PJ.
Comment on 'Generalized approach for accelerated maximum likelihood based image restoration applied to three-dimensional fluorescence microscopy', by L. H. Schaefer, D. Schuster and H. Herz, J. Microsc. 204,
Journal of Microscopy (2002), Vol. 208, Pt 1, pp 1-2

Wouterlood FG, Vinkenoog M and van den Oever M
Tracing tools to resolve neural circuits
Network: Comput. Neural Syst. (2002) 13, pp 327-342

Wouterlood FG, van Haeften T, Blijleven N, Perez-Templado P and Perez-Templado H
Double-Label Confocal Laser-Scanning Microscopy, Image Restoration, and Real-Time Three-Dimensional Reconstruction to Study Axons in the Central Nervous System and Their Contacts With Target Neurons
Applied Immunohistochemistry & Molecular Morphology (2002) 10(1), pp 85-95

Ziese U, Janssen AH, Murk J-L, Geerts WJC, van der Krift T , Verkleij AJ and Koster AJ
Automated high-throughput electron tomography by pre-calibration of image shifts
Journal of Microscopy (2002) Vol 205, Pt2, pp 187-200 get pdf


L, Mittelman L, Tsarfaty I, Shiloh Y and Rotman G
Nuclear Retention of ATM at Sites of DNA Double Strand Breaks
The Journal Of Biological Chemistry (2001) Vol. 276, No. 41, pp

Arnaudeau S, Kelley WL, Walsh JV, Jr. and Demaurex N
Mitochondria Recycle Ca2+ to the Endoplasmic Reticulum and Prevent the Depletion of Neighboring Endoplasmic Reticulum Regions
The Journal Of Biological Chemistry (2001) Vol. 276, No. 31, pp
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Baertschi AJ, Monnier D, Schmidt U, Levitan ES, Fakan S and Roatti A
Acid Prohormone Sequence Determines Size, Shape, and Docking of Secretory Vesicles in Atrial Myocytes
Circ Res.(2001) 89:e23-e29

Bony C, Roche S, Shuichi U, Sasaki T, Crackower MA, Penninger J, Mano H and Pucéat M
A Specific Role of Phosphatidylinositol 3-Kinase g: A Regulation of Autonomic Ca2+ Oscillations in Cardiac Cells
The Journal of Cell Biology (2001) Volume 152, Number 4, pp 717-727 get pdf

Cau J, Faure S, Comps M, Delsert C and Morin N
A novel p21-activated kinase binds the actin and microtubule networks and induces microtubule stabilization
The Journal of Cell Biology (2001) Volume 155, Number 6, pp get pdf

Chen BK, Rousso I, Shim S and Kim PS
Efficient assembly of an HIV-1 MLV Gag-chimeric virus in murine cells
PNAS (2001) vol 98 no26 pp.
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Den Blaauwen T, Lindqvist A, Löwe J and Nanninga N
Distribution of the Escherichia coli structural maintenance of chromosomes (SMC)-like protein MukB in the cell
Molecular Microbiology (2001) 42(5), pp

Guild SB, Murray AT, Wilson ML, Wiegand UK, Apps DK, Jin Y, Rindler M, Roder J and Jeromin A
Over-expression of NCS-1 in AtT-20 cells affects ACTH secretion and storage
Molecular and Cellular Endocrinology (2001) 184, pp 51-63

Habermann FA, Cremer M, Walter J, Kreth G, von Hase J, Bauer K, Wienberg J, Cremer C, Cremer T and Solovei I
Arrangements of macro- and microchromosomes in chicken cells
Chromosome Research (2001) 9, pp 569-584 get pdf

Heun P, Laroche T, Raghuraman MK and Gasser SM
The Positioning and Dynamics of Origins of Replication in the Budding Yeast Nucleus
J Cell Biol (2001) 152, pp 385-400

Lengronne A, Pasero P, Bensimon A and Schwob E
Monitoring S phase progression globally and locally using BrdU incorporation in TK+ yeast strains
Nucleic Acids Research (2001) Vol 29, No 7 pp get pdf

Meesen G and Poffijn A
Semi-automated analysis of three-dimensional track images
Radiation Measurements (2001) 34 pp 161-165

Ogris M, Steinlein P, Carotta S, Brunner S and Wagner E
DNA/polyethylenimine transfection particles: Influence of ligands, polymer size, and PEGylation on internalization and gene expression
AAPS PharmSci (2001) 3 (3) article 21
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Perez-Terzic C, Gacy AM, Bortolon R, Dzeja PP, Puceat M, Jaconi M, Prendergast FG, and Terzic A
Directed Inhibition of Nuclear Import in Cellular Hypertrophy

Prem, C. and Pelster, B.
Swimbladder gas gland cells cultured on permeable supports regain their characteristic polarity
The Journal of Experimental Biology (2001) 204, pp

Rietdorf J, Ploubidou A, Reckmann I, Holmström A, Frischknecht F, Zettl M, Zimmermann T and Way M
Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus
Nature Cell Biology (2001) 3, pp 992 – 1000
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Rohrer, J. and Kornfeld, R.
Lysosomal Hydrolase Mannose 6-Phosphate Uncovering Enzyme Resides in the trans-Golgi Network
Molecular Biology of the Cell (2001) Vol 12, pp
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Rust RC, Landmann L, Gosert R, Luen Tang B, Hong W, Hauri H-P, Egger D and Bienz K
Cellular COPII Proteins Are Involved in Production of the Vesicles That Form the Poliovirus Replication Complex
Journal Of Virology (2001) Vol 75(20), pp

Sander A, Hesser BA and Witzemann V
MuSK induces in vivo acetylcholine receptor clusters in a ligand-independent manner
The Journal of Cell Biology (2001) Volume 155, Number 7, pp

Stanek D, Koberna K, Pliss A, Malínsky J, Masata M, Vecerová J, Risueño M-C and Raska I
Non-isotopic mapping of ribosomal RNA synthesis and processing in the nucleolus
Chromosoma (2001) 110, pp 460-470

Veraverbeke EA, Van Bruaene N, Van Oostveldt P and Nicolaï BM
Non destructive analysis of the wax layer of apple (Malus domestiva Borkh) by means of confocal laser scanning microscopy
Planta (2001) 213, pp 525-533

Verheggen C, Mouaikel J, Thiry M, Blanchard J-M, Tollervey D, Bordonné R, Lafontaine DLJ and Bertrand E
Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domain
The EMBO Journal (2001) Vol.20 N019 pp

Vietor I, Bader T, Paiha K and Huber LA
Peturbation of the tight junction permeability barrier by occludin loop peptides activates b-catenin/TCF/LEF-mediated transcription
EMBO reports (2001) vol 2, no 4, pp 306-312

Vignal E, Blangy A, Martin M, Gauthier-Rouvière C and Fort P
Kinectin Is a Key Effector of RhoG Microtubule-Dependent Cellular Activity

Voermans C, Anthony EC, Mul E, van der Schoot E and Hordijk P
SDF-1-induced actin polymerization and migration in human hematopoietic progenitor cells
Experimental Hematology pp

Walther TC, Fornerod M, Pickergill H, Goldberg M, Allen TD and Mattaj IW
The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins
The EMBO Journal (2001) Vol.20 pp

Wossink J, Karst H, Mayboroda O and Joëls M
Morphological And Functional Properties Of Rat Dentate Granule Cells After Adrenalectomy
Neuroscience Vol. (2001) 108, No. 2, pp 263-272

Wunderlich W, Fialka I, Teis D, Alpi A, Pfeifer A, Parton RG, Lottspeich F and Huber LA
A Novel 14-Kilodalton Protein Interacts with the Mitogen-activated Protein Kinase Scaffold MP1 on a Late Endosomal/Lysosomal Compartment
The Journal of Cell Biology (2001) Volume 152, Number 4, pp 765-776 get pdf


Dukuzumuremyi J-M, Rosqvist R, Hallberg B, Åkerström B, Wolf-Watz H and Schesser K
The Yersinia Protein Kinase A Is a Host Factor Inducible RhoA/Rac-binding Virulence Factor
The Journal Of Biological Chemistry (2000) Vol. 275, No. 45, pp

Geissler H, Ullmann R and Soldati T
The Tail Domain of Myosin M Catalyses Nucleotide Exchange on Rac1 GTPases and Can Induce Actin-Driven Surface Protrusions
Traffic (2000) 1 pp 399-41

Frei C and Gasser SM
The yeast Sgs1p helicase acts upstream of Rad53p in the DNA replication checkpoint and colocalizes with Rad53p in S-phase-specific foci
Genes & Development (2000) 14 pp 81-96

Geissler H, Ullmann R and Soldati T
The Tail Domain of Myosin M Catalyses Nucleotide Exchange on Rac1 GTPases and Can Induce Actin-Driven Surface Protrusions
Traffic (2000) 1 pp 399-410

Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ and Mattaj IW
GTP Hydrolysis by Ran Is Required for Nuclear Envelope Assembly
Molecular Cell (2000) 5: pp
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Hoek M, Engstler M and Cross GAM
Expression-site-associated gene 8 (ESAG8) of Trypanosoma bruceiis apparently essential and accumulates in the nucleolus
Journal of Cell Science (2000) 113, pp get pdf

Huber LA, Fialka I, Paiha K, Hunziker W, Sacks DB, Bähler M, Way M, Gagescu R and Gruenberg J
Both Calmodulin and the Unconventional Myosin Myr4 Regulate Membrane Trafficking Along the Recycling Pathway of MDCK Cells
Traffic (2000) 1: pp 494-503

Jiao J-H, Baumann P, Baron A, Roatti A, Pence RA and Baertschi AJ
Sulfonylurea receptor ligands modulate stretch-induced ANF secretion in rat atrial myocyte culture
Am J Physiol Heart Circ Physiol (2000) 278 pp H2028-H2038

Lang T, Wacker I, Wunderlich I, Rohrbach A, Giese G, Soldati T and Almers W
Role of Actin Cortex in the Subplasmalemmal Transport of Secretory Granules in PC-12 Cells
Biophysical Journal (2000) Volume 78 pp
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Langeveld SMJ, van Wijk R, Stuurman N, Klijne JW and de Pater S
B-type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression
Journal of Experimental Botany (2000) Vol 51, No 349, pp

Loffing J, Pietri L, Aregger F, Bloch-Faure M, Ziegler U, Meneton P, Rossier BC and Kaissling B
Differential subcellular localization of ENaC subunits in mouse kidney in response to high- and low-Na diets
Am J Physiol Renal Physiol (2000) 279: F252-F258

Meyer N, Jaconi M, Landopoulou A , Fort P, Puceat M
A fluorescent reporter gene as a marker for ventricularspecification in ES-derived cardiac cells
FEBS Letters (2000) 478 pp 151-158
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Ogris M, Wagner E and Steinlein P
A versatile assay to study cellular uptake of gene transfer complexes by flow cytometry
Biochimica et Biophysica Acta pp 237-243

Osten P, Khatri L, Perez JL, Köhr G, Giese G, Daly C, Schulz TW, Wensky A, Lee LM and Ziff EB
Mutagenesis Reveals a Role for ABP/GRIP Binding to GluR2 in Synaptic Surface Accumulation of the AMPA Receptor
Neuron (2000) 27, pp 313-325

Prem C, Salvenmoser W, Würtz J and Pelster B
Swim bladder gas gland cells produce surfactant: in vivo and in culture
Am J Physiol Regulatory Integrative Comp Physiol (2000) 279, pp R2336-R2343

Rahner C, Stieger B and Landmann L
Apical endocytosis in rat hepatocytes in situ involves clathrin, traverses a subapical compartment, and leads to lysosomes
Gastroenterology (2000) 119, pp

Rothen-Rutishauser B, Braun A, Günthert M and Wunderli-Allenspach H
Formation of Multilayers in the Caco-2 Cell Culture Model: A Confocal Laser Scanning Microscopy Study
Pharmaceutical Research (2000), Vol. 17, No. 4, pp 460-465

Schwarz EC, Neuhaus EM, Kistler C, Henkel AW and Soldati T
Dictyostelium myosin IK is involved in the maintenance of cortical tension and affects motility and phagocytosis
J Cell Science (2000) 113, pp 621-633

Sönnichsen B, De Renzis S, Nielsen E, Rietdorf J and Zerial M
Distinct Membrane Domains on Endosomes in the Recycling Pathway Visualized by Multicolor Imaging of Rab4, Rab5, and Rab11
J Cell Biology (2000) 149, 4, pp 901-913
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Strohmaier AR, Porwol T, Acker H and Spiess E
Three-dimensional organization of microtubules in tumor cells studied by confocal laser scanning microscopy and computer-assisted deconvolution and image reconstruction
Cells Tissues Organs (2000);167(1) pp 1-8

Stuurman N, Pacios Bras C, Schlaman HRM, Wijfjes AHM, Bloemberg G, and Spaink HP
Use of Green Fluorescent Protein Color Variants Expressed on Stable Broad-Host-Range Vectors to Visualize Rhizobia Interacting with Plants
MPMI (2000) Vol. 13, No. 11 pp
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van der Velden HMW, Ausma J, Rook MB, Hellemons AJCGM, van Veen TAAB, Allessie MA, Jongsma HJ
Gap junctional remodeling in relation to stabilization of atrial fibrillation in the goat
Cardiovascular Research (2000) 46 pp 476-486

Vignal E, De Toledo M, Comunale F, Ladopouloui A, Gauthier-Rouvière C, Blangy A and Fort P
Characterization of TCL, a New GTPase of the Rho Family related to TC10 and Cdc42
The Journal of Biological Chemistry (2000) Vol 275, No 46, pp


Broers JLV, Machiels BM, van Eys GJJM, Kuijpers HJH, Manders EMM, van Driel R and Ramaekers FCS
Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins
J Cell. Science 112, (1999) pp

de Grauw CJ, Vroom JM, van der Voort HTM and Gerritsen HC
Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens
Applied Optics (1999) V 38, No.28, pp

Diaspro A, Corosu M, Ramoino P and Robello M
Adapting a Compact Confocal Microscope System to a Two-Photon Excitation Fluorescence Imaging Architecture
Microscopy Research and Technique (1999) 47, pp 196-205 get pdf

Fialka I, Steinlein P, Ahorn H, Böck G, Burbelo PD, Haberfellner M, Lottspeich F, Paiha K, Pasquali C and Huber LA
Identification of Syntenin as a Protein of the Apical Early Endocytic Compartment in Madin Darby Canine Kidney Cells
The Journal of Biological Chemistry (1999) Vol 274, No 37, pp

Merrifield CJ, Moss SE, Ballestrem C, Imhof BA, Giese G,Wunderlich I and Almers W
Endocytic vesicles move at the tips of actin tails in cultured mast cells
Nature Cell Biology (1999) Vol 1 No 1 pp 72-74
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Oliferenko S, Paiha K, Harder T, Gerke V, Schwärzler C, Schwarz H, Beug H, Günthert U and Huber LA
Analysis of CD44-containing Lipid Rafts: Recruitment of Annexin II and Stabilization by the Actin Cytoskeleton
J Cell Biology (1999) 147, 4, pp 843-854

Porwol T, Strohmaier A-R and Spiess E
Methods in Enzymology (1999) Vol 307 pp 108-118

Verschure PJ, van der Kraan I, Manders EMM and van Driel R
Spatial relationship between transcription sites and chromosome territories
J Cell Biology (1999) 147, 1, pp 13-24

Visser AE and Aten JA
Chromosomes as well as chromosomal subdomains constitute distinct units in interphase nuclei
J Cell Science (1999) 112, pp
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Yang S-H, Larsson O, Brändström R, Bertorello AM, Leiberger B, Leiberger IB, Moede T, Köhler M, Meister B and Berggren P-O
Syntaxin 1 interacts with the LD subtype of voltage-gated Ca2+ channels in pancreatic b cells
Proc Natl Acad Sci USA (1999) Vol 96, pp , Cell Biology


Andersson H, Baechi T, Hoechl M and Richter C
Autofluorescence of living cells
Journal of microscopy (1998) Vol 191 Pt 1, pp 1-7

Buchenau P, Hodgson J, Strutt H and Arndt-Jovin DJ
The Distribution of Polycomb-Group Proteins During Cell Division and Development in Drosophila Embryos: Impact on Models for Silencing
J of Cell Biology (1998) 141, 2, 4 pp 69-481

Rothen-Rutishauser BM, Messerli MJ, van der Voort H, Guenthert M and Wunderli-Allenspach H
Deconvolution Combined with Digital Colocalization Analysis to Study the Spatial Distribution of Tight and Adherens Junction Proteins
Jo. of Computer-Assisted Micr. (1998) V10 No. 3, pp 103-111

Schrader M, Bahlmann K, Giese G and Hell SW
4Pi-Confocal Imaging in Fixed Biological Specimens
Biophysical Journal (1998) Volume 75 pp get pdf

Schrader M, Hell SW and van der Voort HTM
Three-dimensional super-resolution with a 4Pi-confocal microscope using image restoration
Journal Of Applied Physics (1998) Volume 84, Number 8 pp get pdf

Sytsma J, Vroom JM, De Grauw CJ and Gerritsen HC
Time-gated fluorescence lifetime imaging and microvolume spectroscopy using two photon excitation
Journal of Microscopy (1998) Vol 191 Pt1 pp 39-51


Andersson H, Baechi T, Hoechli M, Larsson B, Nievergelt C, Richter C and Swedberg
Autofluorescence as indicator of viability of living cells
XXXIX Symposium of the Society for Histochemistry 24-27 September 1997, Jena, Germany
Histochem Cell Biol (1997) 108 pp 257-285

Hell SW, Schrader M and Van Der Voort HTM
Far-Field fluorescence microscopy with three-dimensional resolution in the 100-nm Range
Journal of Microscopy (1997) Vol 187 Pt1, pp 1-7


Kano H, van der Voort HTM, Schrader M, van Kempen GMP and Hell SW
Avalanche photodiode detection with object scanning and image restoration provides 2-4 fold resolution increase in two-photon fluorescence microscopy
Bioimaging 4 (1996) pp 187-197 get pdf

van Kempen GMP, van der Voort HTM and van Vliet LJ
A quantitative comparison of two restoration methods as applied to confocal microscopy
Journal of Microscopy (1997), Volume 185, no. 3, Pages 354 - 365. Image JoM97GKLVea.pdf


van der Voort HTM and Brakenhoff GJ
3-D image formation in high-aperture fluorescence confocal microscopy: a numerical analysis
Bioimaging 4 (1996) pp 187-197
Journal of Microscopy, April 1990, Volume 158 (1): 43–54
This paper shows the need to take object orientation into account when segmenting or analyzing microscopic images.