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Biological gated-STED data shows resolution of 22nm achievable after Huygens STED deconvolution



We are very grateful to Dr. Grazvydas Lukinavicius (from the laboratory of Prof. Kai Johnsson, LIP-EPFL, Switzerland), who kindly provided us with data obtained with a Leica gated STED system showing 55nm FWHM resolution in the raw data. Keen on achieving even more, Dr. Lukinavicius used Huygens STED deconvolution to reach a blistering 22nm FWHM resolution!!!



Gated STED single plane image and confocal data of a centriole stained with primary and secondary antibody conjugated to Alexa488. Raw and Huygens deconvolved images are shown. Data used by permission from Dr. Grazvydas Lukinavicius, EPFL, Lausanne, Switzerland
Gated STED single plane image and confocal data of a centriole stained with primary and secondary antibody conjugated to Alexa488. Raw and Huygens deconvolved images are shown. Data used by permission from Dr. Grazvydas Lukinavicius, EPFL, Lausanne, Switzerland