Biological gated-STED data shows resolution of 22nm achievable after Huygens STED deconvolution
We are very grateful to Dr. Grazvydas Lukinavicius (from the laboratory of Prof. Kai Johnsson, LIP-EPFL, Switzerland), who kindly provided us with data obtained with a Leica gated STED system showing 55nm FWHM resolution in the raw data. Keen on achieving even more, Dr. Lukinavicius used Huygens STED deconvolution to reach a blistering 22nm FWHM resolution!!!
Gated STED single plane image and confocal data of a centriole stained with primary and secondary antibody conjugated to Alexa488. Raw and Huygens deconvolved images are shown. Data used by permission from Dr. Grazvydas Lukinavicius, EPFL, Lausanne, Switzerland