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News

Biological gated-STED data shows resolution of 22nm achievable after Huygens STED deconvolution

Thursday 21 of February, 2013
We are very grateful to Dr. Grazvydas Lukinavicius (from the laboratory of Prof. Kai Johnsson, LIP-EPFL, Switzerland), who kindly provided us with data obtained with a Leica gated STED system showing 55nm FWHM resolution in the raw data. Keen on achieving even more, Dr. Lukinavicius used Huygens STED deconvolution to reach a blistering 22nm FWHM resolution!

Gated STED single plane image and confocal data of a centriole stained with primary and secondary antibody conjugated to Chromeo488. Raw and Huygens deconvolved images are shown. The intensity profile was fitted to Lorentz distribution. Data used by permission from Dr. Grazvydas Lukinavicius, EPFL, Lausanne, Switzerland
Gated STED single plane image and confocal data of a centriole stained with primary and secondary antibody conjugated to Chromeo488. Raw and Huygens deconvolved images are shown. The intensity profile was fitted to Lorentz distribution. Data used by permission from Dr. Grazvydas Lukinavicius, EPFL, Lausanne, Switzerland

New tutorial videos on Huygens software

Tuesday 05 of February, 2013
Almost all Huygens options are now explained with tutorial videos so that you get easily started with the deconvolution, visualization and analysis options in Huygens.

You can find these videos on this page.

New Huygens Remote Manager (HRM) 3.0 with Colocalization Analysis and Omero file transfers

Tuesday 22 of January, 2013
On Friday January 18, 2013 version 3.0 of the Huygens Remote Manager was released with many new features.

HRM is an open source web interface to Huygens Core for online, multi-user, batch deconvolution and analysis.

Whether the user is at a conference, at home or in the facility itself HRM offers access from anywhere via an internet browser. Your images can now be loaded, deconvolved, analyzed and visualized no matter where you are.

New features

In this version you'll find a new file interface to the open source OMERO server. The interface allows users to collect datasets to be deconvolved from an OMERO server and push back the deconvolution results to it. This is an initial implementation which the HRM developers are planning to extend based on users' feedback.

Secondly, you will find the possibility to use the Huygens Colocalization Analysis on your deconvolved data with great ease of use.

For a detailed list of new features please visit the official page of the HRM project.

Demo server

Feel free to test the new HRM 3.0 in the online HRM demo server.

Huygens version 4.3 released with Crosstalk Corrector

Wednesday 19 of December, 2012
Huygens version 4.3 is now released with many new improvements and a completely new optional Crosstalk Corrector.

A list of the most important improvements can be found on WhatsNew.

Crosstalk (also termed crossover or bleedthrough) can occur when acquiring a Multi Channel image. In that situation, the emission radiation of a given wavelength is detected by the wrong detector because part of the photons go through the wrong optical path inside the microscope (e.g. because the filters efficiency is not 100%). Therefore, some signal is actually recorded as coming from a certain dye when it really comes from a different one.

Crosstalk within Multi Channel images dramatically affects almost any type of data analysis, such as for example CoLocalization. In line with our aim to improve microscopic image quality and measurements, we offer from Huygens version 4.3 onwards the Crosstalk Corrector tool in the Huygens Software to correct for this imaging artifact.
More specific information of the Crosstalk Corrector can be found here

If you like to test the latest Huygens 4.3 version, you can get a free_trial.png of Huygens

Publication on "Colocalization Analysis in Fluorescence Microscopy" describes RBNCC in Huygens

Wednesday 05 of December, 2012
Book Chapter published on "Colocalization Analysis in Fluorescence Microscopy" describes methods of colocalization analysis, and the use of replicate based noise corrected correlation (RBNCC) for improving colocalization analysis of fluorescent microscopic images. The RBNCC method has been developed by the authors of this chapter and is implemented in the Huygens Colocalization Analyzer, as part of a collaboration between the authors and SVI.

Chapter title: Colocalization Analysis in Fluorescence Microscopy
Book title: Cell Imaging Techniques
Series: Methods in Molecular Biology Volume 931, 2013, pp 97-109
Authors Dr. Jeremy Adler and Dr. Ingela Parmryd


For more information click here.

Test licenses for the Huygens RBNCC method and Colocalization Analyzer can be requested by contacting infoImage

SVI and Leica sign contract on Huygens STED deconvolution and analysis

Thursday 23 of August, 2012
Leica and SVI signed an agreement this summer enabling Leica to sell the highly effective Huygens STED deconvolution and analysis software in combination with the advanced Leica STED microscopes. Thanks to close collaboration with the Leica STED development team in Mannheim Germany, Huygens STED deconvolution allows the recovery of amazing details from the raw STED data by increasing the FWHM resolution in X, Y and Z by a factor of two (typical conditions). This resolution boost is combined with a 10-fold increase (typical conditions) in signal strength, greatly facilitating analysis of the data. With these properties, Huygens STED deconvolution makes superresolution microscopy more accessible and of greater practical value to the science community.

Huygens deconvolved image on cover Trends in Pharmacological Sciences.

Thursday 09 of August, 2012
A collaboration between the University of Pittsburgh School of Medicine, and the Massachusetts General Hospital and Harvard Medical School has led to a beautiful cover of the August issue of Trends in Pharmacological Sciences. The cover and accompanying paper, created by the authors J-P. Vilardaga, T.J. Gardella, V.L. Wehbi, and T.N. Feinstein, shows a Huygens deconvolved live early endosome. Click on this link to view the full cover.

Dr. Timothy Feinstein (Department of Pharmacology and Chemical Biology of the University of Pittsburgh School of Medicine, USA) provided us detailed information:
The image is of an early endosome labeled with PTHR-GFP (green), Vps29-YFP (blue) and beta-arrestin 1-dTomato (red), captured using a Nikon A1s spectral confocal at the Nyquist limit, based on the SVI Nyquist calculator, and deconvolved using Huygens Professional. The presentation is a reconstruction of the Huygens output that was alpha blended in Elements AR. Columns represent rotations of the endosome around the vertical axis and rows represent different combinations of color to show colocalization (red/green and blue/green) and lack of colocalization (red/blue).

See Trends in Pharmacological Sciences August issue for the paper.

Huygens 4.2 released with a new STED deconvolution option

Tuesday 12 of June, 2012
The SVI team has released Huygens version 4.2 with many new additions including a brand new STED deconvolution option.

Tests with the LEICA STED microscopes images deconvolved with Huygens STED deconvolution show a huge increase in contrast (~10 times), more resolution in Z, and a significant improvement in the already high lateral STED resolution, revealing spatial detail that can only be seen after deconvolution!

Huygens takes the specific STED parameters into consideration that are reported by the LEICA LIF files. But STED parameters can also be automatically estimated from bead images using the Huygens PSF distiller, which has been specifically extended to fullfil this task.

Batch wise deconvolution of STED images is also possible with the 4.2 Batch Processor.

In addition many other new functionalities have been added to the Huygens software that are available for customers with active licenses.

For example, both the Huygens Essential and Professional 4.2 include a completely new hot pixel removal tool that not only detects but also corrects for hot pixels. The deconvolution wizard has now image comparison tools integrated, and the Colocalization Analyzer is extended with smart background estimators.
We also added to all Huygens basics support of very large tiff files (larger than 4 GB); also known as BigTiffs.
These and all other new features can be viewed at here.

STED deconvolution now available in Huygens

Thursday 23 of February, 2012
Stimulated Emission Depletion (STED) microscopic images can now be deconvolved with the new Huygens STED option with truly stunning results. You can find the latest version at the Download page under development versions.

Developed bij Prof. Stefan Hell and now manufactured by Leica Microsystems, STED microscopes offer true super-resolution. The unique feature of the STED microscope is that it does not have a bandwidth limit, i.e. a barrier beyond which object details are not imaged anymore. While other super-resolution systems are still hampered by this wavelength dependent limit, a STED microscope moves happily beyond it.

Leica now offers two types of STED microscopes of which the CW version allows the usage of all kind of fluophores in the green range thus enabling full biological capabilities.

The Z-resolution, a very important part of biological research, is currently on the level of confocal Z-resolution. By applying Huygens STED deconvolution option you will get a huge increase in contrast (~10 times), more resolution in Z, and also improve the already high lateral STED resolution.

As always, Huygens deconvolution reduces noise and blurring, and takes depth-dependent spherical aberration into account. Geometrical distortions can also be easily corrected in Huygens.



Huygens Confocal and STED deconvolution

Example images obtained from Dr. Juraj Kabat (Biological Imaging Facility, NIH/NIAID, Bethesda, USA), who tested the Huygens STED deconvolution option and commented on the results: "I am really excited" and "it is clearly visible that the STED image is giving much more spatial details, although they are visible only after deconvolution".


Comparision of confocal and STED images, before and after deconvolution with Huygens.
Signal shows regulatory proteins Dmc1 and Rad51 for DNA repair during homologous chromosome recombination in meiosis. Dmc1 shown in green: Mega 520 - 532/685 for ex/em and 770 for depletion. Rad51 shown in red: Atto 647 640/685 ex/em and 770 for depletion. Courtesy of Dr. Juraj Kabat (Biological Imaging Facility, NIH/NIAID, Bethesda, USA.)
Comparision of confocal and STED images, before and after deconvolution with Huygens. Signal shows regulatory proteins Dmc1 and Rad51 for DNA repair during homologous chromosome recombination in meiosis. Dmc1 shown in green: Mega 520 - 532/685 for ex/em and 770 for depletion. Rad51 shown in red: Atto 647 640/685 ex/em and 770 for depletion. Courtesy of Dr. Juraj Kabat (Biological Imaging Facility, NIH/NIAID, Bethesda, USA.)



You are welcome to explore the new Huygens STED option using a free_trial.png version.

Contact Information

Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands


Phone: +31 (0)35 64216 26
Fax: +31 (0)35 683 7971
E-mail: info at svi.nl

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