Suggested beads for widefield PSF acquisition

Recording beads is a necessary first step to distill an experimental PSF. For Widefield microscopes, the following fluorescent beads were used with good results.

TetraSpeck 0.2 µm beads (fluorescent blue/green/orange/red, cat. ref. T-7280) from Life Technologies - Molecular Probes can be used, for example.

Tetraspeck microspheres are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks: 365/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red).

This kind of multicolor beads are very useful to calibrate also the Color Shift.

Contact Information

Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands

Phone: +31 (0)35 64216 26
Fax: +31 (0)35 683 7971
E-mail: info at svi.nl

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