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SVI-Huygens at NIH

Wednesday 20 of September, 2017
SVI is happy to announce that at the end of the year we will be at NIH for the workshop “Huygens Deconvolution, Visualization and Analysis”. The workshop is on Thursday 30th of November, for facility managers and experienced users​, and Friday 1st of December 2017, for all users. The location is room 3LRC1 and 3LRC2, 5601 FISHERS LN, ROCKVILLE, MD 20852.

You can join both in person and via GoToMeeting. For more information and registration, please consult this online form.

We thank the local organizers Dr. J. Kabat and Dr. O. Schwartz, Biological Imaging Facility, RTB/NIAID, for the organization.

WorkshopNIH


The program of the two days is:

November 30, 2017 “Deconvolution and Image Restoration with Huygens”
Experienced users and facility managers
Location: Room 3LRC1 & 3LRC2

  • 9.00 - 9.15 : Welcome– Coffee/Tea

  • 9.15 – 10.00 : Presentation. How to get the best out of your microscopic (super-resolution) images - Huygens image restoration
General introduction to microscopic image formation and restoration. Light/Wave properties, Deconvolution, Point spread function (theoretical vs experimental), imaging issues and pitfalls

  • 10.00 - 10.10 : Short break

  • 10:10 - 11.00 : Huygens Deconvolution (Express and Wizard) and Batch processing
Deconvolve your images with the Deconvolution Express or Wizard, and schedule multiple jobs with the Batch Processor. (With Hands on)

  • 11:00 – 11:20 : Quiz (hands-on)
Find and fix imaging issues

  • 11:20 - 11:35 : Huygens solutions to address many users (simultaneously)
Huygens FLOATING option, Online web interface Huygens Remote Manager with Huygens Core.

  • 11:35 - 12:05 : File formats, data size and handling.

  • 12:05 – 12:25 : Huygens Light Sheet and Fusion wizard
SPIM/Light sheet images can be deconvolved with our new Huygens Light Sheet optical option that takes the specific optical properties of these microscopes into account, as for example SPIM excitation mode, thickness of the sheet, light sheet NA and fill factor and direction. Deconvolution, scattering correction, and restoration (fusion) of multiview SPIM/Light sheet data is combined to facilitate the efficient use of computational resources. (With Hands on)

  • 12:25 - 13:30 : Lunch Break

  • 13:30 – 14:00 : Twin Slicer (hands-on)
Attendees will get some hands-on experience with using the Twin Slicer

  • 14:00 - 14.30 : PSF Distiller
Attendees will get experience with distilling PSFs from bead images, also focusing on STED applications. (With Hands on)

  • 14:30 – 15:00 : Object Stabilizer
Correcting unwanted movement and vibration with the Object Stabilizer. (With Hands on)

  • 15.00 - 15.15 : Short break

  • 15:15 – 16:00 : Chromatic Shift and Cross Talk Correction
How do imaging artifacts affect my analysis? - and how to treat such data.

  • 16:00 - 16:30 : 3D Stitching with deconvolution and automated vignetting correction
The Huygens Stitcher will be demonstrated. Combined automated stitching, deconvolution, and vignetting correction minimizes computer workload and saves time.

  • 16:30 – 17:30 : How to best deconvolve my images: essence of the day
What algorithm should I choose, differences between microscope types, optimal parameter settings, deconvolution artifacts and how to prevent them. With Hands on. The session will be concluded by a discussion on your specific datasets, applications and imaging issues.
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December 1, 2017 “Deconvolution and Image Analysis with Huygens”
All users
Location: Room 3LRC1 & 3LRC2

  • 9.00 - 9.15 : Welcome– Coffee/Tea

  • 9.15 – 10.00 : Presentation. How to get the best out of your microscopic (super-resolution) images - Huygens image restoration
General introduction to microscopic image formation and restoration. Light/Wave properties, Deconvolution, Point spread function (theoretical vs experimental), imaging issues and pitfalls

  • 10.00 - 10.10 : Short break

  • 10:10 - 11.00 : Huygens Deconvolution (Express and Wizard) and Batch processing
Deconvolve your images with the Deconvolution Express or Wizard, and schedule multiple jobs with the Batch Processor. (With Hands on)

  • 11:00 – 11:20 : Quiz (hands-on)
Find and fix imaging issues

  • 11:20 - 11:40 : Short introduction - Chromatic Shift and CrossTalk Correction, and Image Stabilization
How do imaging artifacts affect my analysis? - and how to treat such data.

  • 11:40 - 12:05 : File formats, data size and handling.

  • 12:05 – 12:25 : Huygens Light Sheet and Fusion wizard
SPIM/Light sheet images can be deconvolved with our new Huygens Light Sheet optical option that takes the specific optical properties of these microscopes into account, as for example SPIM excitation mode, thickness of the sheet, light sheet NA and fill factor and direction. Deconvolution, scattering correction, and restoration (fusion) of multiview SPIM/Light sheet data is combined to facilitate the efficient use of computational resources. (With Hands on)

  • 12:25 - 13:30 : Lunch Break

  • 13:30 - 14.00 : Twin and Orthogonal Slicers (with hands-on)

  • 14:00 - 14.45 : MIP, SFP and Surface Rendering (with hands-on)

  • 14:45 – 15.00 : Movie Maker (with hands-on)

  • 15:00 – 15.15 : Huygens solutions to address many users (simultaneously)
Huygens FLOATING option, Online web interface Huygens Remote Manager with Huygens Core.

  • 15.15 - 15.30 : Short break

  • 15:30 – 16:00 – Colocalization Analyzer
How to measure colocalization, which coefficients should I use, and how to do this with Huygens

  • 16:00 – 16:30 : Object Analyzer
Analyzing your microscopy images with the Object Analyzer

  • 16:30 – 17:00 : Object Tracker
How to detect and analyze tracks of moving objects with the Object Tracker

  • 17:00 – 18:00 : How to best deconvolve my images: essence of the day
What algorithm should I choose, differences between microscope types, optimal parameter settings, deconvolution artifacts and how to prevent them. With Hands on. The session will be concluded by a discussion on your specific datasets, applications and imaging issues.