FLIM

What is FLIM?

Fluorescence Lifetime Imaging Microscopy (FLIM) allows you to detect the exponential decay function of the fluorophores. As this decay funtion depends on the environment of the fluorophore, you can even separate fluorophores with the same emission spectrum!

Applications of FLIM

As the lifetime of the fluorophores depend on the environment, you can use FLIM to distinguish different cell types in an organoid without labeling unique features between the cells. For example, FLIM has been use to profile the metabolism of tumours and to determine the efficacy of drugs in organoid cultures.

Restoration of FLIM data

Although Huygens cannot do the lifetime processing to separate the fluorophores based on their lifetime, you can treat the processed images as normal fluorescence data. This means, you can use all image restoration tools in Huygens to improve your images. Set the correct excitation and emission wavelength in the microscopic parameters window and increase the resolution with deconvolution! Also the further analysis of the FLIM data can be done with the Huygens Object Analyzer to obtain robust quantitative data from your FLIM experiments.

Types of FLIM microscope supported

Leica tau-STED