Webinar Schedule

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With these webinars, we like to inform you about the latest Huygens products, and share with you information about how to optimally acquire images, how to recognize artifacts in images, and how to correct for these with Huygens for improved visualization, and more reliable and reproducible quantitative Object and Colocalization analysis.

Upcoming Events:

• Recorded Webinar available: "Autofluorescence and Crosstalk correction with Huygens" (December 3, 2024). Many biological components exhibit autofluorescence, a signal activated by excitation that differs from that of fluorescent probes. While autofluorescence can be useful for studying specific sample properties, it often poses a challenge by obscuring the signal from specific labels. Experimental protocols can help to minimize autofluorescence, such as selecting labels with excitation and emission spectra that do not overlap with the autofluorescent material. However, these measures may not entirely eliminate the issue. Autofluorescence shares similarities with crosstalk, where overlapping excitation and emission spectra from different fluorescent sources cause erroneous signal detection in the wrong channel. As such both issues can be resolved using the same tools. In this webinar, we demonstrate the new Huygens Crosstalk-Autofluorescence Correction option, which is an expansion of the previous Crosstalk Corrector. This new option can correct both crosstalk and autofluorescence in multichannel image data by looking at correlations between signal of multiple channels simultaneaously. Watch the recording here.

• SVI Huygens Virtual Colocalization Workshop (Offered twice as 4-hour sessions on May 15, 2024 to accomodate different time zones). During previous workshops, you may have learned what it takes to become an expert in acquiring and restoring images, and in making them suitable for image analysis. In this workshop, we will delve deeper into obtaining reliable colocalization analysis results from your microscopy data. We will first discuss how resolution and image acquisition can affect image analysis. Through hands-on sessions, you will learn how to use Huygens restoration options to fix acquisition issues that could perturb colocalization analysis. Afterward, we will explain both pixel-based and object-based colocalization, and you will work with images using the Object Analyzer and Colocalization Analyzer in Huygens. Additionally, you will learn how to perform analysis in a batch-wise manner. A workshop manual, demo images, temporary Huygens Everywhere trial will be provided for this workshop. Though not officially announced yet, you can already register for this event by contacting us or sending an email to your SVI account manager.

Held:

• Free Virtual Workshop on "Object Analysis" (Offered twice as 3-hour sessions on October 24, 2024 to accomodate different time zones). During previous workshops, you may have learned what it takes to become an expert in acquiring and restoring images, and in making them suited for image analysis. During this workshop, we go more into detail on how to obtain reliable quantitative information from your microscopy data using Huygens Object Analyzer. You will learn how to segment objects in 3D, use watershedding, apply regions-of-interest, track objects over time, and obtain information about objects withion minutes. Information can include object count, size, shape, surface and intensity. Furthermore, you will see how distances (e.g. nearest neighbor) and colocalization between objects is measured and how analysis can be performed in a batch-wise manner. A workshop manual, demo images, temporary Huygens Everywhere trial will be provided for free for this workshop. Watch the recordings here!.

• Easy on-the-fly spherical aberration corrections using deconvolution (September 3, 2024). Common to imaging thick samples is the distortion and attenuation of structures located deeper within the specimen. This phenomenon stems from the enlargement of the point spread function (PSF) due to spherical aberration, triggered by a mismatch in refractive index (RI) between the sample and the lens immersion medium. Despite efforts to match the RI or use complex hardware solutions, achieving a perfectly spherical aberration-free image is hard to achieve. Fortunately, the Huygens Software offers a simple and effective solution within its deconvolution options! In this webinar, we will explain how to use Huygens for the on-the-fly correction for spherical aberration, and present image data which demonstrates the improvements that can be obtained. Watch this webinar here.

• Microscopic Image Quality Control (July 2, 2024). Ensuring high-quality images is key in obtaining accurate analysis results from microscopy data. However, checking image quality is a difficult and time-consuming task. In our upcoming webinar, we unveil our latest innovation: the Microscopic Image Quality Control feature for Huygens Essential and Professional. This cutting-edge option meticulously checks your images within seconds, detecting a comprehensive range of potential issues. From clipping and hot or cold pixels to crosstalk, chromatic aberration, and metadata discrepancies. No stone is left unturned! Upon analysis, detailed reports are generated, offering clear insights into any identified issues. With intuitive infographics accessible via the 'Help' menu, the findings can be easily understood. Furthermore, options are provided for immediate correction of these issues, ensuring your images are primed for precise analysis. A free version of this QC option is already available online. While this version evaluates images for a subset of issues, it delivers concise reports in a downloadable PDF format, empowering users with valuable insights into their image quality. You can watch the recording here.

• What's new in the upcoming Huygens 24.04 release (May 28, 2024). We are very excited to introduce our brand-new Image Quality Control (QC) option in this Huygens version. The QC option can be added to Huygens Essential and Professional to automatically detect the most common imaging issues, such as bleaching, clipping, hot & cold pixels, crosstalk, chromatic aberration, drift, etcetera. When detected, these issues can be further reviewed and corrected with the corresponding Huygens restoration options (see also our next webinar). Many of you will also appreciate the redesigned user interface of the Object Analyzer and Surface Renderer, which has become much more user-friendly with extendable panels, various new icons for easy navigation, a bar for your favorite buttons, and much more. Within the Object Tracker, it is now easier to detect and fix possible flaws in the tracks and to add missed nodes to tracks. In addition to these new features, the Batch Feeder allows you now to change the depth of subfolders of the input data, to recursively search for images, and to preserve the folder structure of subfolders as found in the input folder in the output folder. Also, the Batch Feeder can automatically run the processing of when it is set to start on startup. To hear more about this and all the other new improvements in Huygens 24.04. You can watch the recording here.

• Huygens Workshop for Imaging Experts (Virtual): "Super-resolution, Quality Control, Quantification, Massive data and Huygens. An update for experts! (April 16+17th, 2024, 2:00-6:00pm CEST). This new Workshop program focuses on microscopists and on imaging specialists (connected to imaging facilities). Even so, we welcome any researcher who is interested in improving and analyzing microscopy images. With presentations and hands-on, we'll dig deeper into deconvolution algorithms, noise correction, image Quality Control, super-resolution images, spherical aberration, imaging thick samples, estimating and correcting imaging artifacts, developing imaging pipelines (Python, command line), colocalization, Huygens & machine learning algorithms, handling large images and datasets, and much more... In addition, we'll present the different Huygens configurations for individual and groups of users. And, we will end with discussing options on how we can help you to train users in imaging and Huygens. Recordings of this workshop will soon be made available.

• What's new in the upcoming Huygens 23.10 release (November 7th, 2023). Besides object-based colocalization analysis, the Workflow Processor can now also perform batch colocalization analysis via the pixel-based approach. Colocalization coefficients can be measured of all channel combinations and time frames, and can be exported to a csv file and as a colocalization map. Huygens 23.10 Object Analyzer offers now the option to import label images created from other segmentation softwares such as the machine-learning based Cellpose and Stardist packages. Imported 2D segmentations can be merged into 3D ones, and we have added Convex Hull-based analysis (see Oct. 24th webinar). A long-term wish to link the segmentation of multiple individual frames to each other is now available. With this option, you can measure how an object changes over time, and produce tracks which can be further analyzed in the Track Analyzer. Image processing is often hampered by limited RAM and disk space. To minimize the size of the result image on disk, we have added a convert data type task in the Workflow Processor. In addition, a new file format, "Memory Mapped File", with extension (.mmf) has been implemented which has the memory buffer that Huygens uses to hold an image residing in a file on disk. This can be used to process images that do not fit in RAM memory. The Stitcher uses this format by default when the result image would otherwise not fit. There is much more in Huygens 23.10 that you will hear about during this webinar. Watch the webinar here.

• Analyzing objects within other objects and object organization, using clusters and convex hull operations (October 24th, 2023). If you image analysis questions concern the organization or properties of objects, or the grouping of objects within other objects such as for example nuclei or cells, then this webinar may interest you. We will show you how tho use the latest features in the Huygens Object Analyzer to not only analyze object in detail, but also to count and analyze the properties of objects within objects (cells, nucleus, organelles) in the other channel. The organization of objects can also be determined within just a single channel by clustering them using the Cluster Analyzer. And, geometric analysis of clusters is facilitated with the newly implemented convex hull option, which basically works like placing a rubber balloon around a cluster of objects. Convex hull based analysis also can aid the analysis of non-homogeneously stained objects, as is often the case with for example DAPI-stained nuclei. The ‘rubber balloon’/convex hull of the object then helps to fill the holes in the segmentation for the non-homogeneous staining. Watch the webinar here.

• Analysis pipelines & Batch Processing; Using Huygens Command Line Interface, Huygens Remote Manager (HRM), or Huygens Worklow Processor (October 10th, 2023). Huygens Core can be configured for maximum performance offered by computer nodes, large scale 64 bit multiprocessors, and systems with multiple (high-end) Nvidia GPU cards. The Huygens Core Compute Engine can be called from any pipeline environment using our new Command Line Interface (CLI). Run all of Huygens' sophisticated image processing tasks, as available in the Workflow Processor, with a node/job managment software such as SLURM (Trademark of SCHEDMD LLC), or from an analysis pipeline scripted with for example Jupyter (Trademark of NumFOCUS foundation). If you do not want to program yourself and are interested in using the Core for batch image processing or with many users, you can use the customizable front-end Huygens Remote Manager. This open source task manager acts as a web-interface to Huygens Core that can be can be used with any web-browser. Aside from these topics, we address during this webinar the new tasks added to the Workflow Processor, such as crosstalk correction and the 3D Object Analyzer. Watch the recording here.

• WhatsNew in the new Huygens 23.04 release (July 4th, 2023). Batch image processing has received much attention with the Huygens 23.04 release! The Workflow Processor contains "Automatic" deconvolution algorithm which does deconvolution with automatically found settings, as in Decon Express. The Workflow Processor now also includes a task for Crosstalk Correction and the option for performing batch Object Analysis. In addition, deconvolution has been improved for images that are acquired under sub-optimal conditions, and images and tables opened in Huygens can now be easily renamed. Huygens 23.04 also includes an update of Python from version 3.7.8 to version 3.11.1. And, via the command line of Huygens Core, it is now possible to apply a workflow template to multiple images, which makes it easy to integrate Huygens deconvolution and processing in your existing custom workflows, and to implement Huygens with a job scheduler (e.g. Slurm). Watch the recording here.

• Huygens User Group Meeting (HUGM) 2023 (June 5th, Noordwijk, The Netherlands). We are pleased to tell you that there will be an in-person Huygens User Group Meeting again. All Huygens customers will receive an invitation for joining this meeting. If you attend, you will hear about all the latest news, have lots of time talking to other Huygens users and our SVI sales and support staff, and have a say during the open discussion. The HUGM is a day before the official start of the ELMI meeting at the 'Strandpaviljoen De Zeemeeuw', which is situated at the beach close to the ELMI venue. The program will start at 17:00 and end with a dinner. Please register on time since the number of spots is limited. More info.

• SVI Huygens Imaging Workshop (Virtual): "How to minimize, recognize, and correct imaging issues in microscopy data (April 25+26th, 2023, 3:00-7:00pm CEST). Hear everything about how to optimally acquire images, how to recognize and correct problems within microscopy data, and how to fix imaging issues. Besides Deconvolution, we will put a focus on other restoration tools including bleaching correction, hot/cold pixel correction, and drift, crosstalk and chromatic aberration correction. Demo images and Huygens Software will be made available. Several presentations will be given addressing the principle of true deconvolution, the PSF structure, and image quantification. Topics will also include: image saving, scaling and file formats. After this course, you will be ready to produce the most beautiful and, more importantly, the most reliable images from your sample! Click here to access the recordings.

• WEBINAR: True restoration of all your STED images with Huygens (March 23, 2023). Whether you are performing STED imaging on an Abberior, Leica or custom-build microscope, optimal STED deconvolution requires a PSF that considers specific STED parameters such as saturation and immunity of the fluorophores, and percentage of 3D depletion. Huygens unique STED theoretical PSF model and PSF distilling option include exactly these parameters. Complemented with state-of-the-art image stabilization and noise handling, Huygens quarantees that you to get the best possible results from your raw STED data. During this webinar, we will also discuss the image feeder and stitching option. Watch the recording here.

• WEBINAR: How to use Huygens Everywhere, and how to manage its use. (March 9, 2023). During this webinar, you will see that Huygens Everywhere is the ideal solution for microscopists who like to use Huygens on whatever online computer that is available, and who like to use it simultaneously. The use of Everywhere will be explained for users and for the Everywhere license owner. In addition, we will demonstrate how the Everywhere owner can easily manage the use of Everywhere via the SVI User Portal, and obtain user statistics. Watch the recording here.

• WEBINAR: Image File formats and Data types (February 23, 2023). 'How should I save my images', a question that is often heard. This Webinar provides an answer to this question, and will also address related topics such as dynamic range, image scaling, bit depth, vendor and open source file formats, data compression, image dimensions, and metadata. In addition;, we will show you that Huygens supports many formats for opening and saving image data, and that Huygens Workflow Processor is the ideal tool for converting image dimensions and file formats. Also in a batch-wise fashion. Join this webinar and become an expert in image file formats! Watch the recording here.

• New Cluster Analyzer in Huygens Localizer for smlm analysis (November 22nd, 2022). Obtain both visual and quantitative information for all kinds of cluster-related research questions. This webinar shows you how to use the FOCAL and DBSCAN algorithm to cluster localizations in Huygens Localizer. Clusters are counted on-the-fly and rendered at the highest possible quality with ray-tracing based iso-surface rendering. You can measure volumes, localization density, width, length, and many other parameters quickly. Clear tool tips and an in-tool tutorial makes that the learning curve is really steep. Watch the recording here.

• WhatsNew in the Huygens 22.10 release (November 8th, 2022). For the first time, Huygens Essential and Professional will have specific deconvolution options for SoRA spinning disc and for VisiTech iSIM data. The Object Analyzer now reports more information about the Region of Interest. The Look-Up Table (LUT) Editor has now an integrated Contrast Editor, and its user interface is now more user-friendly. A new Cluster Analyzer tool is available in Huygens Localizer which uses DBSCAN and FOCAL algorithm to cluster localizations in SMLM data. It also analyzes the found cluster to obtain for example the localization count or length of the clusters. For ease of use, the Cluster Analyzer contains a Tutorial which guides you through the first steps of its use. The clusters can also be analyzed as objects in the Object Analyzer. Watch the recording here..

• Quickly visualize your images for side-by-side comparison and making publication-ready figures. (October 25, 2022). In this webinar, we will explain the new Look-Up Table (LUT)/Contrast Editor in Huygens Twin Slicer and (Twin) MIP renderers. The LUT/Contrast Editor helps you to make easy intensity comparisons between images, map image intensities to colors, and make high quality images. Also, a newly implemented 3D MIP rendering approach ensures the highest degree of artifact-free image projection currently possible - see for yourself! In addition, we'll show you how to export images to make publication-ready material, and how to save your precious images with metadata in a future-proof format. watch the recording here..

• New Quality Control tool for Array (Airyscan) Detector images. Part 2 (October 4, 2022). This webinar will give a short summary of our previous Array Detector (AD) webinar, and we'll demo the new Quality Control tool. This tool automatically calculates and visualizes the relative positions and intensities of each used detector to the central detector. By making optimal use of this position and intensity information, Huygens Array (Airyscan) Detector deconvolution is able to separate objects less than 70nm apart and produce reliable and quantifiable results. We will also show you how to identify incorrect AD image processing. watch the recording here..

• Low signal image data and Huygens; optimize image restoration and acquisition! (September 20, 2022). The significant increase in signal that can be obtained with Huygens deconvolution (can be >8x) not only improves the resolution and contrast within your images, but also allows you to re-design your image acquisition experiment. By taking this approach, you can minimize photo-bleaching and toxicity issues, shorten your time sampling, and image for longer time periods. This will all greatly benefit your (time series) imaging. A special focus will be on image acquisition, super-resolution imaging, and Array (Fast-Airyscan) Detector deconvolution. watch the recording here.

• WhatsNew in the Huygens 22.04 release (June 28th, 2021). Huygens new 22.04 version, which is already downloadable, includes a completely new Look-Up-Table (LUT) Editor, new options in the Object Analyzer and various new features in many of the other Huygens tools. The new LUT editor has been added to the Twin Slicer and (Twin) MIP renderer, and allows the coloring of images using custom-made look-up tables mapping image intensities to colors. It is also possible to load, view and edit Huygens CSV-based LUTs, and existing .lut files. Furthermore, smooth color transitions (gradients) can be applied to specific selections of look-up table elements. Within the Object Analyzer it is now possible to color the objects based on some of their properties and perform cluster analysis with the DBSCAN algorithm. Also the watershed segmentation algorithm has been further optimized to better control how many objects will be split up by the watershed algorithm. For more information you can have a look at Whatsnew and/or watch the recording here.

• Getting the absolute best and true results from your Array (Airyscan) Detector images using Huygens (June 14, 2022). Interested in even faster image acquisition using your Array Detector (incl. the Zeiss Airyscan 1&2) system and getting the absolute best and most reliable results out of these images? If so, then this Webinar may interest you. We will show how you can measure the quality of your specific array detector setup using Huygens Array Analyzer for Quality Control. Also, example images will be shown of typical artifacts that arise when using incorrect restoring approaches for array detector data. Furthermore, Huygens unrivalled Super-sampling mode will be explained which permits you to image much faster at twice the Nyquist pixel size and nonetheless obtain a significant resolution increase. Huygens renowned PSF Distiller is also addressed for obtaining Array Detector PSFs that reflect the specific aberrations of your Array Detector microscope to further optimize the deconvolution results. You can watch the recording here.

• Huygens Virtual Imaging Workshop for the Western Hemisphere (April 20+21, 2022). After the success of our two Virtual Huygens Imaging workshops last year, we decided to organize the same event again, yet now at a time that is more suited for the Western Hemisphere. During this Huygens virtual workshop, you will learn how to acquire the best possible image from nearly any microscope type, and how to use the specialized Huygens features for restoring, visualizing and analyzing your microscopy data in the most reliable manner. Using a Huygens trial version and a collection of demo images, you will get hands-on experience with our world-renowned software.

• Automatic SNR estimation, Acuity and other useful tools in Huygens Deconvolution Wizard (March 29, 2022). With the release of Huygens 21.10.1p0, Deconvolution Wizard has also undergone some major changes. As of now, the deconvolution algorithm and SNR are automatically set to ensure optimal results for each single image. For sure it is possible to edit these settings according to your preference. Deconvolution Preview helps you with adjusting these settings by showing a fast preview of the result within a Region Of Interest (ROI). A slider for adjusting the 'Acuity' has been introduced to further smoothn/sharpen the deconvolution result. This allows you to leave the SNR parameter to its real SNR value. You can watch the recording here.

• New Chromatic Aberration Corrector Wizard (January 20th, 2022). Chromatic aberration causes misalignments and scaling differences between different colors in a microscopy image. Huygens "Christmas Special" (which will be version 21.10.1) introduces corrections for a broader set of possible transformations that could be involved in chromatic aberration. New types of transformations that Huygens can now correct include arbitrary rotation in 3-D, and non-linear radial distortions, of which the barrel-pincushion distortion is the simplest example. There will be a demo of the new chromatic aberration correction tools, and an explanation of which correction is applicable and appropriate in which imaging situations. You can watch the recording here.

• The new WhatsNew in Huygens 21.10 release (November 16th, 2021). Learn from our software developers all about the newly added features, such as Batch Stitching and the possibility to create/edit stitching templates within the Workflow Processor. Use the brand-new Twin MIP Renderer to easily compare 3D volumes side-by-side, and improve visualization of your images within the existing MIP renderer or the new Twin MIP renderer using advanced contrast settings and a heatmap color-mode. Furthermore, include additional channel information with a channel label to each channel, and save/read this info with the image metadata. Huygens Object Analyzer has been expanded with parameters for reporting and counting objects within parent objects in the other channel, and additional ROI and object selection options are now available to facilitate object segmentation. You can watch the recording here.

• Huygens Virtual Imaging Workshop (July 13+14th, 2021). Because of the tremendous success of our 2-day Virtual Huygens Imaging Workshop in April, we have decided to organize the same event again, yet now at a time that is more suited for the Pacific area. During this Huygens virtual workshop, you will learn how to acquire the best possible image and how to use the specialized Huygens features for restoring, visualizing and analyzing your microscopy data. Using a collection of demo images, you will get hands-on experience with our world-renowned software. Registration is closed.

• WhatsNew in Huygens 21.04 release (June 15th, 2021). Distill your array detecor (Airyscan I & II, SPAD) PSFs from bead images, using Huygens PSF Distiller and improve your Array detector data even further. Huygens user-friendly PSF Distiller wizard interface produces high signal PSFs that are ideally suited for deconvolution and for quantifying imaging performance. Quality control of your microscope is facilitated with the automated comparison between distilled PSFs and their theoretical counterparts, and reports can be exported for monitoring changes in image quality. In this webinar, we'll also present a more interactive Deconvolution Express, extended options for Stitching, and the new Workflow Designer tool in the Object Analyzer Advanced. This Workflow Designer offers an interactive block diagram for creating you own analysis pipeline. Watch recording here.

• New Python interpreter and scripting in Huygens Professional (February 9th, 2021). With the new Python interpreter in Huygens Professional, you can access all of our image processing algorithms in a more familiar way. You can use individual commands directly in the Python shell in the main window, or write Python scripts for more complex operations. In this webinar we'll give a quick overview of how to use our Python operations, how to run scripts, and how to use custom modules for even more functionality. Basic Huygens Python operations can be used with little to no knowledge of the Python language, yet a quick refresher may help before checking out this webinar. Watch recording here.

• Huygens new Workflow Processor for batch image processing (January 28th, 2021). If you have many images to process (like from high-content or high-throughput screenings), the new Workflow Processor in Huygens 20.10 comes in very useful. Scheduling many images, or folders of images for processing is easily done within the Workfow Processor, and with the included Task Designer you have a visual overview of all the possible processing steps. More than ten restoration and processing steps are available, such as file conversion, chromatic aberration corection, deconvolution, and drift correction. These processing steps can be easily organised with a drag and drop approach into the active workflow. Once scheduled, the images can be processed and the progress can be monitored with previews of the raw and restored image. Watch recording here.

• Huygens for everyone, everywhere, at any time. Tailored Huygens solutions featuring Huygens Everywhere and the new SVI User Portal (December 1st, 2020). Many researchers already experience every day the flexible use of Huygens via Remotedisplay and the web-based Huygens remote Manager. The new Huygens Everywhere login functionality now makes Essential, Professional and Localizer available on your computer at any time and place. Whether you are at home or in the office, Huygens Everywhere can simply be started with a login as it basically acts like a 'floating license'. Huygens Everywhere is perfectly suited for single users, groups of users, and complete institutes because login credentials and concurrency use can be configured via the new SVI User Portal. Huygens Everywhere is available as a subscription model via our Webshop, and customers with an existing Huygens license can upgrade their license to Huygens Everywhere login use. In this webinar, we'll show what Huygens configuration could be best for you situation, with a focus on Huygens Everywhere, Remote Display, HRM/Huygens Remote Manager and Core, license configurations for single and multiple users, and the use of the new SVI User Portal. Watch recording here.

• WhatsNew in Huygens 20.10 (November 12th, 2020). With the new Workflow Processor in Essential, Professional and Localizer, you can create optimal image processing pipelines easily. Simply drag and drop processing steps into a visual workflow and process many images for batch processing. For example, convert File formats, crop dimensions, and deconvolve and correct drift and chromatic aberration thousands of images at one go. Like Batch Express, the Batch Processor now also includes previews. Accessibiliy of Essential, Professional and Localizer is guaranteed with Huygens Everywhere via a simpel login option. Now, you can configure the use of Huygens Everywhere by multiple users with the new online Huygens Everywhere Portal. The third main feature of the WhatsNew listwhich has been added to Huygens is Python compatibility. All Huygens routines are now accessible via a Python shell and the option to source Python scripts. Watch recording here.

• STEDYCON & HUYGENS: super-resolution scaled up - featuring Huygens Stitcher (June 4, 2020). Do you also struggle with processing tile scan image series, and obtaining large field-of-views at high resolution and free of vignetting issues?.....We can help!.....Sign up now for this webinar in collaboration with Abberior Instruments: STEDYCON & HUYGENS: super-resolution scaled up - featuring Huygens Stitcher. In this webinar, our colleagues at Abberior Instruments briefly introduce the STED technique and will give a live demonstration of the new STEDYCON features, such as multi-positioning and tiling with confocal and STED super-resolution. The acquired images are direectly send to Huygens for seamless tile stitching, deconvolution, vignetting correction, and subsequent image analysis. Michel from SVI will demonstrate the Huygens part of this webinar. Visit also our new Huygens Stitcher webpage, which sumarizes all the great features of the Stitcher, including GPU accelerated registration and deconvolution, and our unique tile position optimalization in X, Y and Z.

• Batch Express: Automated and high quality batch deconvolution for all your microscopy image data (April 7, 2020). Reliable and true Image deconvolution is extremely easy with Huygens! Within a few mouse clicks you have improved your microscopy data. Huygens Batch Express allows fully automated deconvolution of all your microscopy data. By simply defining a 'Watch Folder', you can let Huygens monitor and deconvolve all images files that are saved in this folder. Images can even be saved from a remote (microscope) workstation to this folder to obtain a deconvolved image. Batch processing with deconvolution, chromatic aberration correction, and image stabilization can also be scheduled with templates using Huygens Remote Manager or the Batch Processor, present in Huygens Essential and Professional. Webinar can be viewed here.

• True Deconvolution (March 10, 2020). The real purpose of image restoration is not to produce clean images. It is about finding the existing object that has been imaged. Many image processing approaches aim at clearing images, yet neglect the process of how microscopy images are actually being formed. This is process is also referred to as a convolution. The purpose of TRUE deconvolution is to precisely reverse this convolution process. Since convolution involves object blurring by the microscope type-specific point spread function (PSF), it is crucial that this image building stone is accurately calculated/measured and included in the deconvolution procedure with the most sophisticated deconvolution algorithms currently available. These advanced Maximum Likelihood Estimation (MLE) algorithms are implemented in Huygens with extremely efficient and accurate background and noise correction. Besides deconvolution, Huygens offers a complete restortation toolbox for addressing additional imaging problems. In this webinar, we will explain what true deconvolution involves as opposed to basic image enhancement procedures. We will present example images and also show how to obtain the most reliable and accurate results with Huygens deconvolution. Webinar can be viewed here.

• HRM_HuCore (February 11, 2020). Due to its rapidly increasing user-base, 2019 saw two major releases of the Huygens Remote Manager, the independent browser interface for the Huygens Core. Multi-user, running on all browsers, allowing multiple batch assignments and using the server power in the central facility, The HRM_HuCore is the answer to the ever increasing image-sizes and need for fast high resolution images whenever and wherever you are.This webinar will give you practical guidelines how to optimally use the Huygens Remote Manager with the Huygens Core. Huygens Remote Manager HRM is ideally suited for high-throughput deconvolution and allows access to Huygens via the web-browser, providing an easy multi-user access to Huygens deconvolution. HRM has an optimized interface connection to the open source OMERO server, and also allows you to perform Colocalization Analysis in batch mode and visualize deconvolution results.

• The interactive new Huygens FUSER (28th January 2020). The brand-new interactive Huygens Fuser option can fuse multiview images from a variety of different Light Sheet micrscopes and also allows the deconvolution of the images within its fusion workflow. Alignment of multiview images is very interactive and offers direct visual feedback so that you as a user are in full control.

• Huygens 2D & 3D LOCALIZER webinar (19th November 2019). 2D and 3D Single Molecule Localization Microscopy (SMLM) images acquired with PALM, STORM, GSD, or (DNA)-PAINT can be efficiently analyzed with the Huygens Localizer. Huygens 2D & 3D Localizer is highly parallelized for CPU and GPU, and offers high-quality localization results in real-time. The easy-to-use wizard helps you with the optimization of the detection and pre/post processing settings, and includes various fitting algorithms, powerful background estimation methods, automatic drift correction, and interactive rendering and analysis. New is also the option to batch process SMLM data.

• Huygens 19.10 What's New Webinar (12th November 2019). In this webinar, we will present the new features to be released with the new Huygens 19.10 version, including the latest on the Huygens 2D and 3D Localizer and the interactive Light Sheet fusion (incl. deconvolution).

• 3D Visualization tools (15th October 2019) This webinar gives an overview of the many improvements that we have lately implemented in the three Huygens 3D rendering options, which are the SFP renderer, Surface Renderer, and MIP renderer. All renderers now support GPU acceleration, use perspective projection, and offer more visualization settings. The MIP renderer now supports depth-coded colors, and the Surface Renderer has true cut-away slicing. The SFP renderer has received a big overhaul and is more configurable in terms of both the fluorescence simulation itself as well as the environment. Furthermore, an iso-surface can be placed in the SFP scene with fully realistic shadows and interactions with the fluorescent material, and multiple cutting planes can be added to the SFP scene with arbitrary orientations, allowing you to peer inside the object and produce more information rich animations.

• Stitching in Huygens (September 17th 2019) During this webinar will discuss the Huygens Stitching & Deconvolution wizard. The Stitching & Deconvolution option is GPU accelerated, and offers a unique easy-to-use wizard for combined high-quality stitching for all types of microscopy data with the additional option to deconvolve the images, and to correct for vignetting issues. Vignetting correction can be performed manually and in a full automated fashion. The Stitching & Deconvolution Wizard is available for Huygens Essential and Huygens Professional.

• Huygens LOCALIZER webinar (July 9th, 2019). If you are interested in Single Molecule Localization Microscopy (SMLM) and have PALM, STORM or GSD data to analyse then this webinar might interest you. The new Huygens Localizer is highly parallelized for CPU and GPU, and offers high-quality localization results in real-time. The easy-to-use wizard helps you with the optimization of the detection and pre/post processing settings, and includes various fitting algorithms, powerful background estimation methods, and drift correction & interactive rendering and analysis. You can watch a recording of the webinar on this page.

• Huygens 19.04 What's New Webinar (June 11th, 2019). In this webinar, we presented the new features released with the new Huygens 19.04 version.

• Array Detector (Airyscan&SPAD) webinar - Latest Update (April 30, 2019). This webinar will give you an overview of the kinds of Array Detector systems Huygens supports, which includes all Zeiss Airyscan modes and SPAD systems. This versatile solution will be clearly explained so that you can immediately start with your own SPAD and Airyscan data after the Webinar. Also, we present here the latest Huygens results from Array Detector images provided by our users.

• Huygens STED deconvolution and optimal processing of STED data (March 19, 2019) This webinar will show how you can obtain the best possible results with super-resolution STED image data. STED images can be deconvolved with Huygens leading to high-quality results, and offering a huge increase in contrast and resolution. During this webinar, you will see that Huygens is optimally suited for deconvolving (2D and 3D) STED images from Leica, custom-build STED systems, and the new compact STEDYCON and STED expert line by Abberior Instruments.

• The new HRM with the Huygens Core webinar (29 January 2019). This webinar showcased the functions of the Huygens Remote Manager with the Huygens Core. Huygens Remote Manager HRM is ideally suited for high-throughput deconvolution and allows access to Huygens via the web-browser, providing an easy multi-user access to Huygens deconvolution. HRM has an optimized interface connection to the open source OMERO server, and also allows you to preform Colocalization Analysis in batch mode and visualize deconvolution results.

• Huygens 18.10 What's New Webinar - revisited (January 15, 2019). On many requests a rerun of this webinar. It will showcase the new features that are included in the 18.10 Huygens version. To mention three high-lights we introduce a fully new Huygens solution that has been made for all types of Array Detector microscopes (e.g. Zeiss Airyscan and SPAD scopes). Plus a super-intuitive colocalization solution including both Object Colocalization as well as full Colocalization via coefficients. A third highlight is the possibility to work with multiple extremely large GPU cards in Huygens.

• Array Detector (Airyscan&SPAD) Webinar (November 27th, 2018). This webinar will give you an overview of all kinds of Array Detector systems Huygens supports, which includes all types of Airyscan modes that Zeiss built into its microscopes plus the various SPAD modes. This versatile solution will be clearly explained so that you can immediately start with your own Airyscan data after the Webinar.

• Huygens 18.10 What's New Webinar (November 13, 2018). This webinar will showcase the new features that are included in the 18.10 Huygens version. To mention three high-lights we introduce a fully new Huygens solution that has been made for all types of Array Detector microscopes (e.g. Zeiss Airyscan and SPAD scopes). Plus a super-intuitive colocalization solution including both Object Colocalization as well as full Colocalization via coefficients. A third high-light is the possibility to work with multiple extremely large GPU cards in Huygens.

• Reliable quantification of fluorescence images: from raw data to reproducible results (9th of October 2018): In this webinar, you will learn how to overcome issues with stage drift and crosstalk between channels, how to obtain reliable images and reproducible quantification results, how to proceed with advanced image analysis after restoring images. During fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blurring and the noise implicit in the image formation are two of the largest sources of experimental trouble. Additional aberrations, such as stage drift, and crosstalk and chromatic aberration between channels, can also affect the imaging. Huygens image deconvolution and restoration is a proven method to revert these issues and recover a more realistic representation of the original object. After restoration of the image, you can proceed with the advanced Huygens analysis options for colocalization, object measurements, and tracking. This webinar will illustrate how you can make optimal use of the complete Huygens workflow to obtain reliable images and reproducible quantification results, focusing on the advanced analysis options offered by the Huygens software. You can view a recording of this webinar after October 15th, if you register on this SVI- BiteSizeBio webpage.

• Light-Sheet/SPIM Deconvolution&Fusion by Huygens (18th of September, 2018) This webinar describes the latest developments in Light-sheet/SPIM deconvolution and fusion in Huygens. If you join you'll get a general overview plus an thorough update on developments since the introduction in Spring 2017. You will learn about the specific aspects of deconvolution of Light-sheet data combined with Fusion and scattering correction via the Huygens Wizard.

• Object Analysis with Huygens (3rd of July 2018): Huygens deconvolution improves resolution, contrast and signal to noise ratio in the images. Because of this, the analysis of the deconvolved images is more accurate and robust. The analysis can be performed using the Huygens Object Analyzer. The interactive object analyzer tool for 3D microscopy images in Huygens Essential and Huygens Professional allows you to obtain statistics of individual objects by clicking on them, or analyzing all objects with a single button press. The image is segmented, the objects can be filtered and grouped in Regions of Interest, and the images are then analyzed for Geometrical data, Spatial location, Relation with neighbors, Relation with reference objects, Degree of overlap (colocalization).

• Huygens Co-localization webinar (19th June 2018) Colocalization studies provide an important insight on several biological processes, and Huygens can help you in getting the most accurate colocalization results. Huygens deconvolution improves resolution, contrast and signal to noise ratio, reducing the effect of Blur And Noise in Colocalization studies. Our Restoration Options also correct the images for the other aberrations, getting them ready for an accurate analysis. For what concerns the analysis, the Colocalization Analyzer works more at the level of the whole image, and the Object Analyzer also provides colocalization measurements at the object level. In this webinar, we will discuss: image restorations before co-localization analysis and why, a brief introduction of the co-localization coefficients, how to use the Huygens co-localization Analyzer (demo).

• Huygens 18.04 What's New Webinar (30th May 2018)This webinar will showcase the new features that are included in the 18.04 Huygens version. In the new version we will introduce a new feature in Huygens: the Batch Express! You simply have to select the desired Watched folder and the images in the folder will be automatically deconvolved by the Batch Express within seconds. The Batch Express supports a wide range of microscope types (widefield, confocal, spinning disk, multiphoton, STED, and a variety of SPIM/Light Sheet systems) and file formats. Compatibility with your image files is not an issue. Additional features and tools in the 18.04 version are: Triplet mode in Twin Slicer (in Basic Mode!), Added “Cold Pixel” mode in Hot Pixel Remover, New Lattice Light Sheet Deconvolution, Object Stabilizer: added deskew mode for Lattice Light Sheet data, ability to choose a reference frame or slice, Added colocalization coefficients in an improved Coloc Analyzer: Li's ICQ, Van Steensel's CCF, Native Nikon ND2 and Olympus OIR readers for Windows, Multi GPU deconvolution via the Operations Window in Huygens Professional, New GPU operations: filters, copy, statistics, histogram operations, slicing, multiMIP, faster deconvolution, visualization, and Deconvolution Express, New right-click menu for an easier workflow

• Get the Best Out of Your Microscopy Images with Huygens Batch Express (24th April 2018) . In this webinar, you will learn about the advantages of deconvolving all your images fully automatically. Huygens high-quality deconvolution is now accessible with a fully automated image-processing pipeline within the new Batch Express option. You simply have to select the desired Watched folder and the images in the folder will be automatically deconvolved by the Batch Express within seconds. The Batch Express supports a wide range of microscope types (widefield, confocal, spinning disk, multiphoton, STED, and a variety of SPIM/Light Sheet systems) and file formats. Compatibility with your image files is not an issue. This Webinar is organized in collaboration with BiteSizeBio. Join us on the BiteSizeBio SVI-Huygens webinar about the Batch Express the 24th of April 2018 at London time 4 PM, Boston time 11 AM, San Diego time 8 AM here.

• Huygens webinar for users of Leica microscopes (11th April 2018) . This webinar is targeted for the users of Leica microscopes from all over the word. This webinar will help you to ensure high quality Huygens deconvolution of your Leica files. Join us the 11th of April to know more about: Huygens combined with the new LASX version from 2018 onwards. What remains the same, and what changes?; Huygens as stand alone package to improve your results further. What is present already that you may not know about?; Possibilities to extend Huygens deconvolution to all your types and brands of microscopes. With this webinar, we are looking forward to providing you more insights on your Huygens package.

• Stitching in Huygens (30th January 2018) This webinar will describe the Huygens Stitching & Deconvolution wizard. The Stitching & Deconvolution wizard is GPU accelerated, and offers a unique easy-to-use option for combined high-quality stitching for all types of microscopy data with the additional option to deconvolve the images, and to correct for vignetting issues. Vignetting correction can be performed manually and in a full automated fashion. The Stitching & Deconvolution Wizard is available for Huygens Essential and Huygens Professional.

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