How to calculate the X and Y sample sizes using a CCD camera.

Find out the pixel sizes of the camera's CCD chip. Typical values are 6.7 x 6.7 micron. With a 25X objective the sample sizes measure 6700/25 = 268 nanometer.

This is assuming that there is no extra magnification, but some microscope models have an internal magnification that must also be considered. In that case, the total magnification (all magnifications multiplied) must be used when calculating the pixel size.

Keywords: sample size CCD
Categories: Faq Microscopy, Huygens Faq, Imported Faqs
Platforms: Irix Linux Windows Mac AIX
Related products: Hu Ess Hu Pro