Huygens Tutorials
This page lists useful Huygens Software tutorials. There are also tutorial movies for exploring Huygens.
Acquisition
Good imaging starts at acquisition, way before image processing.
- Important Factors for quality restoration
- Acquisition Pitfalls
- Setting the good sampling: Nyquist Calculator
Restoration
What and what not is Image Restoration. Restoration by Doing Deconvolution.
Essential
- How to use the Huygens Essential wizard: Essential Starting Tutorial
- Step by step explanation: Essential Deconvolution Roadmap. Some relevant steps are
- Batch Processor and Batch Processor Tutorial
- Deconvolution For Non Optical Images
PSF
The Point Spread Function (PSF) contains the most relevant information describing your optical device.
- Recording Beads to obtain an experimental PSF
- Tutorial on Using The Psf Distiller
- Step by step explanation: Psf Distillation Roadmap
Deconvolution examples
With detailed explanations on the different parameters involved.
- Example of a Deconvolution Procedure
- More on Tuning Huygens Deconvolution
- How to deconvolve STED images
Visualization and interactive analysis
- Twin Slicer and Twin Slicer Tutorial
- MIP renderings and animations: The MIP Renderer
- Realistic 3D rendering: Sfp Renderer
- Surface Renderer and Surface Renderer Tutorial
- Movie Maker and Movie Maker Tutorial
- Colocalization Analyzer and Colocalization Tutorial and Colocalization Coefficients
- Object Analyzer and Object Analyzer Tutorial
Programming
- Introduction to Tcl-Huygens commands
- How to program Huygens: Scripting Course
- Some examples: Hu Tcl Useful Procedures
- Restoration batch jobs: Batch Script